We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
FUJIREBIO

altona Diagnostics GmbH

altona Diagnostics GmbH develops and commercializes real-time PCR based assays and molecular diagnostic test solution... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
18 Mar 2021 - 21 Mar 2021
13 Apr 2021 - 16 Apr 2021

Prolonged Maternal Zika Viremia Evaluated for Adverse Perinatal Outcomes

By LabMedica International staff writers
Posted on 10 Feb 2021
Print article
Image: The RealStar Zika Virus RT-PCR Kit 1.0 is an in vitro diagnostic test, based on real-time PCR technology, for the qualitative detection of Zika virus specific RNA in human serum or urine (Photo courtesy of Altona Diagnostics).
Image: The RealStar Zika Virus RT-PCR Kit 1.0 is an in vitro diagnostic test, based on real-time PCR technology, for the qualitative detection of Zika virus specific RNA in human serum or urine (Photo courtesy of Altona Diagnostics).
The recent worldwide epidemic confirmed maternal–fetal transmission of Zika virus (ZIKV) and its association with adverse perinatal outcomes, particularly severe central nervous system lesions and fetal losses.

ZIKV is detectable in maternal blood by reverse transcription polymerase chain reaction (RT-PCR) during the acute phase of infection. ZIKV viremia usually lasts from two days before to 16 days after symptom onset. The median time of ZIKV RNA clearance is five days.

A team of medical scientists led by those at Lausanne University Hospital (Lausanne, Switzerland) carried out a prospective cohort study in French Guiana and enrolled Zika virus–infected pregnant women with a positive PCR result at inclusion and non-infected pregnant women; both groups underwent serologic testing in each trimester and at delivery during January–July 2016. Prolonged viremia was defined as ongoing virus detection >30 days post-infection.

The team performed molecular and serologic testing using the RealStar Zika Kit (Altona Diagnostics GmbH, Hamburg, Germany) for RT-PCR, in-house IgM and IgG antibody-capture ELISA, and microneutralization assays for serologic testing. The limit of detection for serum samples tested using the RealStar Zika Kit was 0.61 copies/μL (95% CI 0.39–1.27). A cycle threshold (Ct) value <37 was considered positive.

The team reported that adverse outcomes (fetal loss or neurologic anomalies) were more common in fetuses and neonates from mothers with prolonged viremia (40.0%) compared with those from infected mothers without prolonged viremia (5.3%, adjusted relative risk [aRR] 7.2 [95% CI 0.9–57.6]) or those from non-infected mothers (6.6%, aRR 6.7 [95% CI 3.0–15.1]). Congenital infections were confirmed more frequently in fetuses from mothers with prolonged viremia (9/15 [60.0%]) when compared with those from infected mothers without prolonged viremia (5/19 [26.3%], RR 2.3 [95% CI 1.0–5.4]) and non-infected mothers (0/332 [0.0%]).

The authors concluded that prolonged maternal ZIKV viremia could be a marker for an increased risk for maternal–fetal transmission and subsequent adverse perinatal outcomes. Even if prolonged maternal viremia is not consistently present in cases of congenital infection, it might reflect active viral replication in the fetal–placental compartment and should lead to an enhanced prenatal and neonatal follow-up. The study was published in the February 2021 issue of the journal Emerging Infectious Diseases.

Related Links:
Lausanne University Hospital
Altona Diagnostics GmbH


New
Gold Supplier
SARS-CoV-2 Assay
SARS-CoV-2 UTAB FS
SARS-CoV-2 Nucleic Acid Detection Kit
SARS-CoV-2 Nucleic Acid Detection Kit
New
Hematology Analyzer
DxH 560 AL
SARS-CoV-2 RT-PCR Test
CareGene N-CoV RT-PCR Kit

Print article

Channels

Molecular Diagnostics

view channel
Image: Histopathologic image from a patient with Li Fraumeni syndrome showing accumulation of mutant TP53 in tumoral cells (Photo courtesy of Thierry Soussi, PhD).

Clinical Germline Testing Evaluated for Pediatric Cancer Patients

Pediatric cancer is rare, with fewer than 10,000 solid tumors diagnosed in children annually in the USA. Previous studies interrogating germline predisposition broadly across pediatric cancer types have... Read more

Industry

view channel
Illustration

Mindray and Tencent AI Lab to Jointly Develop AI Products for Blood Disease Screening and In Vitro Diagnosis

Shenzhen Mindray Biomedical Electronics Co., Ltd. (Shenzhen, China) has signed an AI cooperation framework agreement with Tencent AI Lab (Shenzhen, China) to jointly develop AI products for blood cell... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.