We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us


Seegene, Inc. is a developer of multiplex molecular technologies and multiplex clinical molecular diagnostics for inf... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
18 Mar 2021 - 21 Mar 2021
13 Apr 2021 - 16 Apr 2021

TAP1 Gene Polymorphisms Associated with Recurrent Respiratory Papillomatosis

By LabMedica International staff writers
Posted on 09 Feb 2021
Print article
Image: The Anyplex II HPV28 Detection Kit simultaneously detects and identifies 28 HPV types (19 high-risk and 9 low-risk) (Photo courtesy of Seegene).
Image: The Anyplex II HPV28 Detection Kit simultaneously detects and identifies 28 HPV types (19 high-risk and 9 low-risk) (Photo courtesy of Seegene).
Recurrent respiratory papillomatosis (RRP) is a respiratory tract disease that affects children and adults and is characterized by the recurrent proliferation of multiple papillomas. The proliferation of multiple papillomas within the airway in RRP primarily affects the larynx.

RRP is a potentially devastating and incurable disease, caused by human papillomavirus (HPV) infection. A dysfunctional immune response in patients with RRP has been reported. A low expression of the transporter associated with antigen processing (TAP) and major histocompatibility complex class I (MHC‐I) genes in benign papillomas has been found.

Medical Microbiologists at the Universidad de Guadalajara (Guadalajara, México) and their colleagues recruited 35 patients who were diagnosed with papillomatosis. Biopsies for histopathologic and molecular diagnosis were taken during surgical resection. DNA extraction was performed for the screening and genotyping of HPV. Blood samples were collected from 35 unrelated control subjects and 35 patients with RRP. To confirm the presence or absence of HPV in papilloma tissue, a PCR analysis was performed.

Genotype identification and semi‐quantitative determinations of viral load were performed by Multiplex Real‐Time PCR, using the Anyplex HPV28 Detection Kit (Seegene, Seoul, South Korea), with the CFX96 real‐time thermocycler (Bio‐Rad, Hercules, CA, USA). Polymorphisms were identified using primers and FastStart Taqman hydrolysis probes for each of the single nucleotide polymorphism (SNP) in their wild‐type and polymorphic forms, using TAP1 gene ID rs11352216. The amplification reaction was carried out in a StepOne Thermal Cycler (Applied Biosystems, Waltham, MA, USA).

The scientists reported that after HPV screening and genotyping, the most frequent genotypes were HPV‐6 and HPV‐11. HPV‐6 was present in 21 of 35 patients, and HPV‐11 was present in only 14 patients. Of the 21 patients with HPV‐6, 15 presented with high viral loads, whereas six presented with intermediate‐low viral loads and five of the 14 patients with HPV‐11 had a high viral load and nine had an intermediate‐low viral load.

In the polymorphism analysis, rs1057141 showed no significant differences. In contrast, a significant difference was found in rs1135216 (Odds Ratio, [OR] = 2.4) in the allelic analysis, as well as in the dominant (OR = 3.06), codominant (OR = 3.06), and additive model, OR = 2.505) in subjects with the G allele.

The authors concluded that their findings suggest that the rs1135216 A/G polymorphism in TAP1 is associated with a risk for RRP, and that there is a possible risk related to the rs1057141 A/G polymorphism in a western Mexican population. The study was published on January 28, 2021 in the Journal of Clinical Laboratory Analysis.

Related Links:
Universidad de Guadalajara
Applied Biosystems

Gold Supplier
Sample Transport
VACUETTE Transport Line
Flu + SARS Antigen Test
Sofia 2 Flu + SARS Antigen FIA
SARS-CoV-2 Antigen Test
2019-nCoV Antigen Test (Lateral Flow Method)
Gold Supplier
Tumor Marker Control
Tumor Marker Control

Print article


Molecular Diagnostics

view channel
Image: Histopathologic image from a patient with Li Fraumeni syndrome showing accumulation of mutant TP53 in tumoral cells (Photo courtesy of Thierry Soussi, PhD).

Clinical Germline Testing Evaluated for Pediatric Cancer Patients

Pediatric cancer is rare, with fewer than 10,000 solid tumors diagnosed in children annually in the USA. Previous studies interrogating germline predisposition broadly across pediatric cancer types have... Read more


view channel

Mindray and Tencent AI Lab to Jointly Develop AI Products for Blood Disease Screening and In Vitro Diagnosis

Shenzhen Mindray Biomedical Electronics Co., Ltd. (Shenzhen, China) has signed an AI cooperation framework agreement with Tencent AI Lab (Shenzhen, China) to jointly develop AI products for blood cell... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.