We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company


Illumina develops, manufactures and markets integrated systems for the analysis of genetic variations and biological ... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

ALL Genomes Marked by High DNA Methylation Levels

By LabMedica International staff writers
Posted on 24 May 2022
Print article
Image: The TruSeq Methyl Capture EPIC Library Prep Kit (Photo courtesy of Illumina)
Image: The TruSeq Methyl Capture EPIC Library Prep Kit (Photo courtesy of Illumina)

Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer and consists of multiple subtypes with distinct gene expression profiles defined by constellations of somatic mutations, chromosomal rearrangements deregulating oncogenes or encoding chimeric fusion transcripts and aneuploidy.

DNA methylation is tightly regulated. during development and is stably maintained in healthy cells. In contrast, cancer cells are commonly characterized by a global loss of DNA methylation co-occurring with CpG island hypermethylation. In ALL, the commonest childhood cancer, perturbations of CpG methylation have been reported.

A large team of medical scientists led by the Max Planck Institute for Molecular Genetics (Berlin, Germany) obtained diagnosis leukemia samples from children treated in St. Jude Children’s Research Hospital (Memphis, TN, USA). They performed whole-genome bisulfite sequencing across ALL subtypes, leukemia cell lines and healthy hematopoietic cells, and show that unlike most cancers, ALL samples exhibit CpG island hypermethylation, but minimal global loss of methylation.

Whole-genome, transcriptome and exome sequencing was performed using Illumina exome baits and library preparation and Illumina Hi-Seq or NovaSeq sequencers (Illumina, San Diego, CA, USA). Whole-genome bisulfite sequencing (WGBS) of primary patient samples and all cell lines except Jurkat and DND41 was performed on a bisulfite-modified DNA-sequencing library generated by the Illumina TruSeq DNA Methylation kit.

The team reported that T-ALL exhibited a global DNA methylation landscape comparable to precursor T cells derived from healthy infant thymi, whereas B-ALL samples showed mild loss of methylation at varying degrees. ALL samples exhibit CpG island hypermethylation, but minimal global loss of methylation. This was most pronounced in T cell ALL and accompanied by an exceptionally broad range of hypermethylation of CpG islands between patients, which are influenced by TET2 and DNMT3B.

The authors concluded that their study provides important data and insights into the non-canonical epigenetic regulation of ALL as well as cancer more generally that, combined with recent studies, helps to focus attention on particular aspects of the cancer methylome and will help move toward an improved mechanistic understanding of the common epigenetic changes in cancer. The study was published on May 19, 2022 in the journal Nature Cancer.

Related Links:
Max Planck Institute for Molecular Genetics 
St. Jude Children’s Research Hospital 

Platinum Supplier
Hormonal Test
Endocrine Array
Direct LDL Assay
Direct LDL-C
Chlamydia Infection Diagnostic Test
Chlamydia Test
Blood Transfusion Safety Test
ABTest Card

Print article



view channel
Image: Trichinella sp. found in muscle tissue (Photo courtesy of McGill University)

Laboratory Features of Trichinellosis and Eosinophilia Threshold Determined

Trichinella nativa is a nematode worm, one of the species of the genus Trichinella, found in arctic and subarctic regions. It is highly pathogenic and has a high resistance to freezing. It is encapsulated,... Read more


view channel
Image: With Cell IDx’s acquisition, Leica Biosystems will be moving its multiplexing menu forward (Photo courtesy of Leica Biosystems)

Leica Biosystems Acquires Cell IDx, Expanding Offerings in Multiplexed Tissue Profiling

Leica Biosystems, a technology leader in automated staining and brightfield and fluorescent imaging (Nussloch, Germany), has acquired Cell IDx, Inc. (San Diego, CA, USA), which provides multiplex staining... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.