We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company

THERMO FISHER SCIENTIFIC

  Gold Thermo Fisher Scientific provides analytical instruments, lab equipment, specialty diagnostics, reagents and integrat... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Biochemical Abnormalities Among Celiac Disease Patients Referred for Antibody Testing

By LabMedica International staff writers
Posted on 09 May 2022
Print article
Image: The Phadia 250 Immunoassay Analyzer, in addition to allergy testing, allows measurement of autoantibodies of more than 20 autoimmune related diseases using the EliA product line (Photo courtesy of Thermo Fisher Scientific)
Image: The Phadia 250 Immunoassay Analyzer, in addition to allergy testing, allows measurement of autoantibodies of more than 20 autoimmune related diseases using the EliA product line (Photo courtesy of Thermo Fisher Scientific)

Celiac disease (CD) is a chronic disease occurring in all age groups and affecting approximately 1% of the population, although many cases of CD remain undiagnosed. This condition is caused by an abnormal immune response in genetically susceptible individuals triggered by the ingestion of gluten proteins from wheat, rye and barley.

Celiac disease primarily affects the small intestine, often leading to malabsorption and micronutrient deficiencies. A small intestinal biopsy with recognition of villus atrophy and inflammation has been the gold standard for diagnosis; however, serological testing is increasingly used in the diagnostic process and screening for CD, mainly by the detection of CD-specific antibodies.

Clinical Scientists at the Copenhagen University Hospital (Copenhagen, Denmark) and their colleagues included in an observational cohort study 706 individuals that had received a positive CD antibody result; 72.7% were women and the mean age was 26 years. The team compared the results of those with CD-positive antibodies with individuals with CD-negative antibodies.

Tissue transglutaminase antibody (IgA) (TTG-IgA), tissue transglutaminase antibody (IgG) (TTG-IgG), deamidated gliadin peptide antibody (IgA) (DGP-IgA) and deamidated gliadin peptide antibody (IgG) (DGP-IgG) were measured in serum by fluorescence enzyme immunoassay (EIA) on the UniCAP 100 and ImmunoCAP 250 platforms (Phadia Laboratory Systems, Thermo Fisher Scientific, Hvidovre, Denmark).

Other variables used from the CopLab database were hemoglobin, erythrocytes, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), transferrin, hematocrit, ferritin, alanine transaminase (ALAT), alkaline phosphate, 25-OH vitamin D, folic acid, cobalamin, C-reactive protein (CRP), reticulocyte, mean corpuscular hemoglobin (ReticMCH), erythrocyte volume, relative distribution width (RDW), and immunoglobulin A.

The investigators reported a most remarkable difference between the groups was the markedly lower ferritin among CD antibody-positive individuals compared with CD antibody-negative individuals (women: 13.8 µg/L versus. 35.9 µg/L; men: 34.3 µg/L versus 80.4 µg/L), Also, CD antibody-positive individuals had a tendency for lower hemoglobin (women: 7.8 mmol/L versus 8.1 mmol/L; men: 8.5 mmol/L versus 8.8 mmol/L). The team reported lower cobalamin and folic acid levels and higher levels of transferrin, alanine transaminase and alkaline phosphate among CD antibody-positive individuals.

Compared with CD-negative individuals, the scientists reported that a greater proportion of tests among CD antibody-positive individuals exhibited hemoglobin (10.2% versus 2.7%), mean corpuscular volume (7.1% versus 2.9%), mean corpuscular hemoglobin concentration (6.8% versus 1.2%) and ferritin (37.6% versus7.6%) below the reference level, while transferrin (20.7% versus 9.5%) was above the reference interval. CD antibody-positive individuals were also more likely to have a deficiency for cobalamin and folic acid.

Line Lund Kårhus, MD, PhD, the lead author of the study, said, “This study identified several biochemical abnormalities associated with celiac disease (CD) antibody positivity in a primary care setting among individuals referred to CD antibody testing. The pattern of abnormalities suggested that micronutrient deficiencies were prevalent among CD antibody-positive individuals.”

The authors concluded that their study showed more measurements below the reference interval for hemoglobin, MCV, MCHC, ferritin, cobalamin and folic acid among the individuals with a positive CD antibody test. The pattern of the included biomarkers suggested that micronutrient deficiencies were common among CD antibody-positive individuals and confirmed malabsorption as a sign of CD. The study was published on April 18, 2022 in the journal Scientific Reports.

Related Links:
Copenhagen University Hospital 
Phadia Laboratory Systems 

Gold Supplier
Quality Control Material
Multichem U
New
Silver Supplier
Helicobacter Pylori Test
AB HP UREASE TEST
New
Gold Supplier
Chemistry Analyzer
BS-600M
New
Silver Supplier
Anti-Müllerian Hormone (AMH) ELISA Test
PCOCheck ELISA (CE)

Print article

Channels

Molecular Diagnostics

view channel
Image: This image depicts a DNA molecule that is methylated on both strands on the center cytosine. DNA methylation plays an important role for epigenetic gene regulation in development and cancer (Photo courtesy of Wikimedia Commons)

Study Supports Use of Methylated DNA Biomarkers for Cancer Diagnosis and Prognosis

A recent study added weight to the theory that methylated DNA biomarkers could be used for cancer diagnosis and prognosis. Methylation is a biological process by which methyl groups are added to a DNA molecule.... Read more

Hematology

view channel
Image: Standard test for multiple myeloma provides clues of a rare, more deadly type (Photo courtesy of Pexels)

Standard Blood Cancer Test Provides Clues of Rare IgD Multiple Myeloma

IgD myeloma accounts for about 1% of common blood cancer multiple myeloma and has a worse prognosis. Specific testing for IgD myeloma is available at a handful of reference labs across the US, but takes... Read more

Industry

view channel
Image: QIAGEN has acquired a majority stake in enzymes provider BLIRT S.A. (Photo courtesy of QIAGEN)

Qiagen Acquires Enzymes Provider Blirt to Strengthen Sample Technologies Business

QIAGEN N.V. (Venlo, Netherlands) has signed agreements to acquire a 96% majority ownership stake in BLIRT S.A. (Gdansk, Poland), a manufacturer of recombinant enzymes for the life science industry.... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.