We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us


Thermo Fisher Scientific provides analytical instruments, lab equipment, specialty diagnostics, reagents and integrat... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Biochemical Abnormalities Among Celiac Disease Patients Referred for Antibody Testing

By LabMedica International staff writers
Posted on 09 May 2022
Print article
Image: The Phadia 250 Immunoassay Analyzer, in addition to allergy testing, allows measurement of autoantibodies of more than 20 autoimmune related diseases using the EliA product line (Photo courtesy of Thermo Fisher Scientific)
Image: The Phadia 250 Immunoassay Analyzer, in addition to allergy testing, allows measurement of autoantibodies of more than 20 autoimmune related diseases using the EliA product line (Photo courtesy of Thermo Fisher Scientific)

Celiac disease (CD) is a chronic disease occurring in all age groups and affecting approximately 1% of the population, although many cases of CD remain undiagnosed. This condition is caused by an abnormal immune response in genetically susceptible individuals triggered by the ingestion of gluten proteins from wheat, rye and barley.

Celiac disease primarily affects the small intestine, often leading to malabsorption and micronutrient deficiencies. A small intestinal biopsy with recognition of villus atrophy and inflammation has been the gold standard for diagnosis; however, serological testing is increasingly used in the diagnostic process and screening for CD, mainly by the detection of CD-specific antibodies.

Clinical Scientists at the Copenhagen University Hospital (Copenhagen, Denmark) and their colleagues included in an observational cohort study 706 individuals that had received a positive CD antibody result; 72.7% were women and the mean age was 26 years. The team compared the results of those with CD-positive antibodies with individuals with CD-negative antibodies.

Tissue transglutaminase antibody (IgA) (TTG-IgA), tissue transglutaminase antibody (IgG) (TTG-IgG), deamidated gliadin peptide antibody (IgA) (DGP-IgA) and deamidated gliadin peptide antibody (IgG) (DGP-IgG) were measured in serum by fluorescence enzyme immunoassay (EIA) on the UniCAP 100 and ImmunoCAP 250 platforms (Phadia Laboratory Systems, Thermo Fisher Scientific, Hvidovre, Denmark).

Other variables used from the CopLab database were hemoglobin, erythrocytes, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), transferrin, hematocrit, ferritin, alanine transaminase (ALAT), alkaline phosphate, 25-OH vitamin D, folic acid, cobalamin, C-reactive protein (CRP), reticulocyte, mean corpuscular hemoglobin (ReticMCH), erythrocyte volume, relative distribution width (RDW), and immunoglobulin A.

The investigators reported a most remarkable difference between the groups was the markedly lower ferritin among CD antibody-positive individuals compared with CD antibody-negative individuals (women: 13.8 µg/L versus. 35.9 µg/L; men: 34.3 µg/L versus 80.4 µg/L), Also, CD antibody-positive individuals had a tendency for lower hemoglobin (women: 7.8 mmol/L versus 8.1 mmol/L; men: 8.5 mmol/L versus 8.8 mmol/L). The team reported lower cobalamin and folic acid levels and higher levels of transferrin, alanine transaminase and alkaline phosphate among CD antibody-positive individuals.

Compared with CD-negative individuals, the scientists reported that a greater proportion of tests among CD antibody-positive individuals exhibited hemoglobin (10.2% versus 2.7%), mean corpuscular volume (7.1% versus 2.9%), mean corpuscular hemoglobin concentration (6.8% versus 1.2%) and ferritin (37.6% versus7.6%) below the reference level, while transferrin (20.7% versus 9.5%) was above the reference interval. CD antibody-positive individuals were also more likely to have a deficiency for cobalamin and folic acid.

Line Lund Kårhus, MD, PhD, the lead author of the study, said, “This study identified several biochemical abnormalities associated with celiac disease (CD) antibody positivity in a primary care setting among individuals referred to CD antibody testing. The pattern of abnormalities suggested that micronutrient deficiencies were prevalent among CD antibody-positive individuals.”

The authors concluded that their study showed more measurements below the reference interval for hemoglobin, MCV, MCHC, ferritin, cobalamin and folic acid among the individuals with a positive CD antibody test. The pattern of the included biomarkers suggested that micronutrient deficiencies were common among CD antibody-positive individuals and confirmed malabsorption as a sign of CD. The study was published on April 18, 2022 in the journal Scientific Reports.

Related Links:
Copenhagen University Hospital 
Phadia Laboratory Systems 

Gold Supplier
Automatic Western Blot Analyzer
Tenfly Phoenix Blot Analyzer
AFP Detection Kit
Alpha-Fetoprotein (AFP) Rapid Test
TSH (Thyroid Stimulating Hormone) Test
Kemilo TSH Kit
Fecal Bacterial Test
CLART EnteroBac

Print article


Molecular Diagnostics

view channel
Image: Researchers have identified the origin of subset of autoantibodies that worsen lupus (Photo courtesy of Pexels)

Lupus Biomarker Testing Could Help Identify Patients That Need Early and Aggressive Treatment

Systemic lupus erythematosus (SLE) is an autoimmune disease that occurs when the body's antibodies, which usually protect against infections, attack healthy cells and proteins. These autoantibodies can... Read more


view channel
Image: A genetic test could guide the use of cancer chemotherapy (Photo courtesy of Pexels)

Genetic Test Predicts Whether Bowel Cancer Patients Can Benefit From Chemotherapy

Late-stage bowel cancer patients usually undergo a series of chemotherapies and targeted medicines for cancer treatment. However, the responses to the last-line chemotherapy treatment trifluridine/tipiracil... Read more


view channel
Image: Use of DBS samples can break barriers in hepatitis C diagnosis and treatment for populations at risk (Photo courtesy of Pexels)

DBS-Based Assay Effective in Hepatitis C Diagnosis and Treatment for At Risk Populations

In a bid to eliminate viral hepatitis as a public health threat by 2030, the World Health Organization (WHO) has put forth a proposed strategy. To this end, researchers at the Germans Trias i Pujol Research... Read more


view channel
Image: New research has opened a path for fast and accurate cancer diagnosis (Photo courtesy of Imagene)

AI-Based Image Analysis Software Profiles Cancer Biomarkers in Real Time

Lung cancer is the most widespread type of cancer worldwide, resulting in approximately 1.76 million fatalities annually. Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancer diagnoses... Read more


view channel
Image: Live view of non-fluorescent specimens using the glowscope frame (Photo courtesy of Winona State University)

Device Converts Smartphone into Fluorescence Microscope for Just USD 50

Fluorescence microscopes are utilized to examine specimens labeled with fluorescent stains or expressing fluorescent proteins, like those tagged with green fluorescent protein. However, since these microscopes... Read more


view channel
Image: The global antimicrobial resistance diagnostics market size is expected to reach USD 5.7 billion by 2028 (Photo courtesy of Pexels)

Global Antimicrobial Resistance Diagnostics Market Driven by Increasing Hospital-Acquired Infections

Antimicrobial drugs are intended to counteract the harmful effects of microbes and promote a healthy life. However, their excessive use can result in the development of resistance, commonly referred to... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.