We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Primary Graft Failure Identified by Early Chimerism Following DCBU Transplantation

By LabMedica International staff writers
Posted on 14 Apr 2022
Print article
Image: Umbilical cord blood has increased access to potentially curative therapy for patients with life-threatening disorders of the bone marrow and immune system (Photo courtesy of Cellsafe Biotech Group)
Image: Umbilical cord blood has increased access to potentially curative therapy for patients with life-threatening disorders of the bone marrow and immune system (Photo courtesy of Cellsafe Biotech Group)

Umbilical cord blood transplantation (UCBT) has increased access to potentially curative therapy for patients with life-threatening disorders of the bone marrow and immune system. The introduction of reduced intensity conditioning (RIC) regimens and double umbilical cord unit infusions (DUCBT) has broadened the applicability of UCBT to frailer or larger recipients.

This procedure has led to potentially curative allogeneic transplantation being accessed by thousands of patients with diseases of the bone marrow and immune system who would otherwise have been precluded for lack of a suitably HLA matched sibling or adult volunteer unrelated donor. The kinetics of chimerism after RIC DUCBT and their clinical utility are poorly understood.

Hematologists at the University College London Hospital's NHS Foundation Trust (London, UK) and their colleagues recruited 58 patients with a median age of 52 years, with overall and progression-free survivals of 59% and 52%, respectively, at two years. Non-relapse mortality was 4% at day 100, and the relapse rate was 31% at one year. Peripheral blood lineage-specific chimerism was feasible from day 7 after transplant onward.

Lineage-specific chimerism studies (peripheral blood mononuclear cells [PBMCs], B cells, T cells, and granulocytes) were performed according to local laboratory procedures on 5 to 10 mL EDTA peripheral blood samples on days 7, 14, 21, 28, 35, 60, and 100, 6 months, and 1 and 2 years to determine the relative contribution of donor and recipient to overall hematopoiesis. Complete donor chimerism was defined as marrow reconstitution of at least 90% donor origin.

The scientists reported that five patterns of chimerism were observed including (1) complete single unit dominance (39 patients), (2) sustained donor-donor mixed chimerism (three patients), (3) sustained donor-recipient mixed chimerism (five patients), (4) dominance reversion (one patient), and (5) primary graft failure (four patients). The RIC CBT trial enabled adult patients with high-risk hematologic malignancies to safely access UCBT in the UK and provided novel insights into the kinetics of donor and recipient chimerism after RIC DUCBT that are clinically relevant. Of the 21 patients who relapsed overall, 15 had stable chimerism before relapse, including 11 patients with acute leukemia, two with Hodgkin lymphoma, one with follicular lymphoma, and one with multiple myeloma.

The authors concluded that in 81% of patients achieving sustained donor engraftment in their study, a single UCB became the dominant contributor to hematopoiesis with eradication of the second UCB unit and residual recipient bone marrow, usually within the first 35 days after transplant. The study provided novel insights into the kinetics of donor and recipient chimerism after RIC DUCBT that are clinically relevant. The study was published on April 12, 2022 in the journal Blood Advances.

Related Links:
University College London Hospital's NHS Foundation Trust 

HLX
Gold Supplier
ESR Analyzer
miniiSED™
New
C-Reactive Protein Test
STANDARD F hs-CRP FIA
New
Helminth Multiplex PCR Assay
Allplex GI-Helminth(I) Assay

Print article

Channels

Clinical Chem.

view channel
Image: Electrochemical cells etched by laser on wooden tongue depressor measure glucose and nitrite in saliva (Photo courtesy of Analytical Chemistry)

Biosensor-Fabricated Wooden Tongue Depressor Measures Glucose and Nitrite in Saliva

Physicians often use tongue depressors to examine a patient's mouth and throat. However, it is hard to imagine that this simple wooden tool could actively assess a patient's health. This idea has led to... Read more

Immunology

view channel
Image: Newly observed anti-FSP antibodies have also been found to predict immune-related adverse events (Photo courtesy of Calviri)

First Blood-Based Biomarkers Test to Predict Treatment Response in Cancer Patients

Every year worldwide, lung cancer afflicts over two million individuals and almost the same number of people succumb to the disease. This malignancy leads the charts in cancer-related mortalities, with... Read more

Microbiology

view channel
Image: The rapid MTB strip test for tuberculosis can identify TB patients within two hours (Photo courtesy of Chulalongkorn University)

Rapid MTB Strip Test Detects Tuberculosis in Less Than an Hour without Special Tools

Tuberculosis (TB), a highly infectious disease, continues to pose significant challenges to public health worldwide. TB is caused by a bacterium known as "Mycobacterium tuberculosis," spreading through... Read more

Pathology

view channel
Image: The UNIQO 160 (CE-IVDR) advances diagnostic analysis for autoimmune diseases (Photo courtesy of EUROIMMUN)

Novel Automated IIFT System Enables Cutting-Edge Diagnostic Analysis

A newly-launched automated indirect immunofluorescence test (IIFT) system for autoimmune disease diagnostics offers an all-in-one solution to enhance the efficiency of the complete IIFT process, comprising... Read more

Technology

view channel
Electronic biosensor uses DNA aptamers for detecting biomarkers in whole blood samples (Photo courtesy of Freepik)

Electronic Biosensor Detects Biomarkers in Whole Blood Samples without Addition of Reagents

The absence of robust, reliable, and user-friendly bioanalytical tools for early and timely diagnosis of cardiovascular diseases, particularly sudden cardiac arrest, leads to preventable deaths and imposes... Read more

Industry

view channel
Image: The global HbA1c testing devices market is expected to reach USD 2.56 billion in 2027 (Photo courtesy of Freepik)

Global Hemoglobin A1c Testing Devices Market Driven by Rising Prevalence of Diabetes

Hemoglobin A1c (HbA1c), or glycated hemoglobin, refers to hemoglobin with glucose attached. HbA1c testing devices are used for blood tests that determine average blood glucose, or blood sugar levels.... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.