We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

SYSMEX-EUROPA

Sysmex Europe designs and produces laboratory and hematology diagnostic solutions, including instruments, reagents, c... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Exhaled Nitric Oxide and Eosinophil Count Diagnose Eosinophilic Asthma

By LabMedica International staff writers
Posted on 01 Sep 2021
Print article
Image: Blood film showing eosinophilic leukocytes (single cell arrowed) surrounded by red blood cells. About 50% of people with severe asthma may have elevated levels of eosinophils in their blood (Photo courtesy of Jane Liesveld, MD)
Image: Blood film showing eosinophilic leukocytes (single cell arrowed) surrounded by red blood cells. About 50% of people with severe asthma may have elevated levels of eosinophils in their blood (Photo courtesy of Jane Liesveld, MD)
Eosinophilic asthma is now recognized as an important subphenotype of asthma based on the pattern of inflammatory cellular infiltrate in the airway. Eosinophilic asthma can be associated with increased asthma severity, atopy, late-onset disease, and steroid refractoriness.

Asthma is characterized by recurrent respiratory symptoms and a variable expiratory-airflow limitation, affecting approximately 334 million people worldwide. Tests to identify reversible airflow limitation are important in asthma diagnosis, but they are time-consuming and it may be difficult for patients to cooperate.

Respiratory Medicine specialists at the Daping Hospital, Army Medical University (Chongqing, China) conducted a retrospective cohort study on 2,349 suspected asthma cases between January 2014 and December 2019. Fractional exhaled nitric oxide (FeNO) was evaluated with an online measurement technique using a Nano Coulomb nitric oxide analyzer. FeNO results were reported as parts per billion (ppb), and FeNO measurements were performed prior to spirometry, the methacholine challenge test, and the reversibility test.

The investigators took peripheral venous blood samples, and blood eosinophils (B-Eos) and leukocytes were counted using a Sysmex XN‐9000 Hematology Analyzer (Sysmex, Kobe, Japan), a multifunctional automatic hematology analyzer and leukocyte classifier. B-Eos counts were reported along with other leukocyte subpopulations, and the percentage of each subpopulation was calculated. Baseline spirometry, the bronchial-provocation and bronchodilation test (BPT, BDT) were performed using a Jaeger spirometer (Erich Jaeger GmbH, Würzburg, Germany).

The scientists reported that the main study population included 897 males and 1,452 females, of whom 824 patients were diagnosed with asthma. Asthmatic patients had significantly higher white blood cell counts (7.24 versus 7.05 × 109/L), B-Eos counts (306 versus 105 cells/μL), B-Eos percentages (4.5% versus 1.8%), and FeNO levels (52 versus 5 ppb). The percentage of blood neutrophils, forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), and FEV1/FVC ratio of asthmatic patients were significantly lower (59.3% versus 61.9%, 94.4% versus 96.2%, 79.7% versus 92.4%, and 68.5% versus 80.2%), respectively. The odds ratio for having asthma increased progressively with a gradual increase in FeNO or B-Eos count.

The authors concluded that there was no difference in diagnostic accuracy for asthma between FeNO and B-Eos count, and the combination of these two biomarkers could slightly improve diagnostic efficacy. Patients with moderately elevated biomarkers (FeNO > 40 ppb and B-Eos > 300 cells/μL) could be diagnosed with asthma and avoid objective tests when such tests are not feasible. The study was published on August 9, 2021 in the journal BMC Pulmonary Medicine.

Related Links:
Daping Hospital, Army Medical University
Sysmex
Erich Jaeger GmbH


New
Gold Supplier
Desktop Urine Analyzer
MONOZYME TX2000
New
Gold Supplier
Microfluidic Analyzer
MONOFLOW ZX5100
New
Centrifuge
iFuge M5K Combi
New
3-Part Hematology Analyzer
HL 3125 plus

Print article

Channels

Clinical Chem.

view channel
Image: Illustration is of the Vertical Auto Profile (VAP) Lipid test with clear demarcation of the different lipoprotein classes and subclasses. (Photo courtesy of VAP Diagnostics Laboratory)

Lipoprotein(a) Concentrations Correlate With LDL-C in Diabetic Children

Cardiovascular disease (CVD) is a significant cause of mortality in those with diabetes. Increased apolipoprotein B (apoB) and low-density lipoprotein cholesterol (LDL-C) have been shown in pediatric patients... Read more

Molecular Diagnostics

view channel
Image: Model of the PD-1 (Programmed cell death protein 1) protein. Only a subset of recurrent glioblastomas respond to anti-PD-1 immunotherapy (Photo courtesy of Wikimedia Commons)

Biomarker Predicts Potential Benefit of Checkpoint Inhibitor Therapy for Brain Cancer Patients

A phosphorylated form of ERK (extracellular signal-regulated kinase) protein has been identified as a biomarker that may be used to predict which brain cancer patients might benefit from checkpoint inhibitor... Read more

Immunology

view channel
Image: Procartaplex Immunoassays Kits are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously (Photo courtesy of Thermo Fisher Scientific)

Assay Developed for Patient-Specific Monitoring and Treatment for Ovarian Cancer

Tumors can influence peripheral immune macroenvironment, thereby creating opportunities for non-invasive serum/plasma immunobiomarkers for immunostratification and immunotherapy designing.... Read more

Microbiology

view channel
Image: Clinical metagenomics (CMg) using nanopore sequencing (Photo courtesy of Oxford Nanopore Technologies)

Same Day Test Identifies Secondary Infections in COVID-19 Patients

The intensive care unit (ICU) is a dynamic environment with frequent staff-patient contact for invasive monitoring, interventions and personal care that together introduce the risk of secondary or nosocomial... Read more

Pathology

view channel
Image: The Ventana BenchMark Ultra autostainer is for cancer diagnostics with automation and the test menu include IHC, ISH, and FITC tests (Photo courtesy of Ventana Medical System)

Specific Biomarker Investigated for Triple-Negative Breast Cancer Diagnosis

Triple-negative breast cancer (TNBC) is defined by the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression and comprises a heterogeneous... Read more

Technology

view channel
Image: PKeye Workflow Monitor System (Photo courtesy of PerkinElmer, Inc.)

PerkinElmer’s New Cloud-Based Platform Enables Laboratory Personnel to Remotely Manage Its Instruments in Real-Time

PerkinElmer, Inc. (Waltham, MA; USA) has launched its PKeye Workflow Monitor, a cloud-based platform enabling laboratory personnel to remotely manage and monitor their PerkinElmer instruments and workflows... Read more

Industry

view channel
Illustration

Global HBA1c Laboratory Tests Market Driven by Rise in Diabetic Population

The global HBA1c laboratory tests market is projected to expand at a significant pace over the coming years, driven by an increase in the prevalence of diabetes, rise in prescription rate of HBA1c tests... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.