We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Flow Cytometry Detects Myelodysplasia in Peripheral Blood

By LabMedica International staff writers
Posted on 27 Mar 2018
Print article
Image: The BD FACSCanto II flow cytometer analyzer (Photo courtesy of BD Biosciences).
Image: The BD FACSCanto II flow cytometer analyzer (Photo courtesy of BD Biosciences).
Myelodysplastic Syndromes (MDS) are a group of myeloid neoplasms characterized by inefficient hematopoiesis, peripheral blood (PB) cytopenias and high risk of leukemic progression.

The diagnosis of MDS is essentially based on morphological and cytogenetic abnormalities, such as the presence of cytopenias, blasts in the PB and/or bone marrow (BM), and dysplasia in one or more hematopoietic cell lineages. Although PB cytomorphological findings provide information to suspect of MDS, only BM studies are presently accepted to confirm the diagnosis.

Scientists associated with the Hospital de Santo António Porto (Porto, Portugal) studied 14 patients with low-risk MDS (LR-MDS), eight males and six females, with a median age of 76 years, ranging from 66 to 88 years and that had at least one appointment at the hospital from September 2015 to November 2015. An equal number of healthy blood donor controls were studied in parallel, eight males and six females, with a median age of 55 years, ranging 19 to 63 years.

Clinical and laboratory data were retrospectively collected from the hospital records. Bone marrow (BM) aspirate samples were used to prepare BM smears. These were stained with Leishman’s stain, and cell morphology was analyzed by conventional light microscopy. Cytogenetics studies were performed on BM aspirates, either by conventional cytogenetics and/or fluorescence in situ hybridization (FISH).

Peripheral blood samples were collected into ethylene-diamine-tetra-acetic acid tripotassium salt containing tubes and processed within 24 hours after collection. Cell immunophenotyping was performed by 8-color flow cytometry (FCM) using fluorochrome-conjugated monoclonal antibodies (mAbs) with different specificities. Sample acquisition was performed in a BD FACSCanto II flow cytometer.

The team found that peripheral blood neutrophils from patients with LR-MDS frequently had low forward scatter (FSC) and side scatter (SSC) values and low levels of CD11b, CD11c, CD10, CD16, CD13 and CD45 expression, in that order, as compared to normal neutrophils. In addition, patients with LR-MDS commonly display a higher fraction of CD14+CD56+ and a lower fraction of CD14+CD16+ monocytes in the PB. The immunophenotyping score based on which PB samples from patients with LR-MDS could be distinguished from normal PB samples with a sensitivity of 93% and a specificity of 100%.

The authors concluded that their pilot study revealed an altered neutrophil immunophenotype, often accompanied by an abnormal monocyte immunophenotype, in the PB from nearly all LR-MDS cases, and suggest that assessment of abnormal antigen expression in PB mature myeloid cells may help to identify patients with LR-MDS. Once translated into a straightforward FCM-based score and converted into a visual analogue scale, MDS thermometer, these findings can be easily applied in clinical practice. The study was published on March 13, 2018, in the journal BMC Hematology.

Related Links:
Hospital de Santo António Porto

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.