We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Roche Diagnostics

Develops, manufactures, and markets a wide range of in vitro diagnostic systems, instruments, reagents, and tests read more Featured Products: More products

Download Mobile App




Elevated PlGF Levels Found with Acute Fatty Liver of Pregnancy

By LabMedica International staff writers
Posted on 22 Jul 2021
Print article
Image: The ROCHE Cobas 6000 Chemistry Analyzer (Photo courtesy of Roche Diagnostics)
Image: The ROCHE Cobas 6000 Chemistry Analyzer (Photo courtesy of Roche Diagnostics)
Acute fatty liver of pregnancy (AFLP) is a severe liver disorder unique to pregnancy, typically occurring after 30 weeks’ gestation. Although uncommon, with an estimated global incidence of 1 in 7,000 to 15,000 pregnancies, AFLP is a life-threatening disease for both mother and child.

During preeclampsia, poor placentation triggers the excessive release of the soluble vascular endothelial growth factor receptors (VEGFR); also known as soluble Fms-like tyrosine kinase-1 (sFlt-1), which binds its free circulating ligands VEGF and placental growth factor (PlGF). The ensuing angiogenic imbalance is thought to contribute significantly to the clinical manifestations of this disorder.

Physicians at the Erasmus MC University Hospital (Rotterdam, the Netherlands) and their colleagues utilized human serum samples from a database of women with singleton pregnancies who had a clinical diagnosis of AFLP between 2005 and 2020. The team compared matched women with AFLP to 12 gestational age (GA) matched women with either no preeclampsia, confirmed preeclampsia, or HELLP syndrome, given that the values of sFlt-1, free, and total PlGF alter with advancing gestation.

Measurement of sFlt-1, Free PlGF, and Total PlGF was carried out. For the thermal dissociation of all sFlt-1-PlGF complexes, serum samples were placed in a heating block at 70 °C for 10 minutes. Measurements of sFlt-1 and PlGF before and after heating were performed using the automated Elecsys immunoassay from Roche Diagnostics (Cobas 6000, e-module; Rotterdam, the Netherlands).

The scientist found that median levels of free PlGF were significantly lower in 13 women with preeclampsia (117pg/mL) or in 12 women with hemolysis elevated liver enzymes and low platelet count syndrome (59 pg/mL) compared with 11 women without preeclampsia (349 pg/mL). In contrast, median total PlGF did not differ between women with no preeclampsia, preeclampsia, and hemolysis elevated liver enzymes and low platelet count syndrome (354 pg/mL versus 435 pg/mL versus 344 pg/mL) respectively, whereas it was markedly elevated in AFLP compared with all groups (2,054 pg/mL). Furthermore, in AFLP, both sFlt-1 and total PlGF declined rapidly post-delivery, with significantly higher in 12 pre-delivery total PlGF (median, 2,054 pg/mL) than 14 postpartum levels (median, 163 pg/mL), suggesting that in AFLP, PlGF is largely placenta-derived.

The authors concluded that their findings indicated that like sFlt-1, PlGF production is significantly upregulated in AFLP, mainly originating from the placenta. Importantly, total PlGF can now be easily calculated from already available free PlGF and sFlt-1 levels, allowing subsequent evaluation of other groups in whom PlGF is altered. The study was published on June 28, 2021in the journal Hypertension.

Related Links:
Erasmus MC University Hospital
Roche Diagnostics


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
POCT Fluorescent Immunoassay Analyzer
FIA Go
Gold Member
Xylazine Immunoassay Test
Xylazine ELISA

Print article

Channels

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.