We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us

Download Mobile App





NIH Develops Robust SARS-CoV-2 Serology ELISAs from Serum and Dried Blood Microsamples

By LabMedica International staff writers
Posted on 08 Jan 2021
In order to achieve the high levels of specificity and sensitivity needed for its "serosurvey" to track undetected cases of COVID-19 in the US, researchers at the National Institutes of Health (NIH Bethesda, MA, USA) have developed two assays to detect the spike protein and receptor binding protein, achieving sensitivity of 100% (95% CI 76.8%, 100%), and specificity of 100% (95% CI 96.4%, 100%).

In its paper on its serology study for development of an ELISA that detects SARS-CoV-2 antibodies with 99-100% specificity, the NIH’s protocol defines initial thresholds for IgG and IgM antibodies to determine seropositivity from both clinical and at-home blood samples using the Mitra device from Neoteryx LLC (Torrance, CA, USA), with a reduced risk of false positives. More...
This protocol is key to helping NIH researchers and scientists in other labs and research organizations confidently determine the extent to which the coronavirus has spread undetected, which communities are most affected, and who has developed SARS-CoV-2 antibodies.

The NIH researchers are sharing this information so other laboratories can replicate the steps for SARS-CoV-2 antibody testing using both serum and Mitra blood microsamples for similar studies. As a next step, NIH research teams at NIAID, NIBIB, NCATS and NCI are analyzing thousands of blood microsamples collected by citizens using Mitra devices in at-home Mitra Blood Collection Kits.

"The NIH ELISA-based serology protocol using Mitra microsampling devices with VAMS technology from Neoteryx gives all scientists a reliable method for identifying SARS-CoV-2 raised antibodies in blood microsamples with amazing specificity, which is critical during the coronavirus pandemic," said James Rudge, PhD, Technical Director, Neoteryx. "Many tests that were rushed out early in the COVID-19 crisis, did not have the high sensitivity and high specificity necessary to reliably detect key antibodies that indicate an immune response specific to COVID-19 versus some other type of coronavirus. The ELISA protocol from the NIH enables SARS-CoV-2 immunity studies that deliver accurate data, and we're very proud that our Mitra microsampling devices with VAMS technology supported this impressive achievement."

Related Links:
The National Institutes of Health (NIH)
Neoteryx LLC



Gold Member
SARS‑CoV‑2/Flu A/Flu B/RSV Sample-To-Answer Test
SARS‑CoV‑2/Flu A/Flu B/RSV Cartridge (CE-IVD)
Portable Electronic Pipette
Mini 96
New
Sample Transportation System
Tempus1800 Necto
New
Gel Cards
DG Gel Cards
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Molecular Diagnostics

view channel
Image: Colorectal cancer under the microscope (Photo courtesy of Adobe Stock)

Unique Microbial Fingerprint to Improve Diagnosis of Colorectal Cancer

Colorectal cancer is the fourth most common cancer in the UK and the second deadliest. New research has revealed that it carries a unique microbial fingerprint, which could help doctors better understand... Read more

Hematology

view channel
Image: New research points to protecting blood during radiation therapy (Photo courtesy of 123RF)

Pioneering Model Measures Radiation Exposure in Blood for Precise Cancer Treatments

Scientists have long focused on protecting organs near tumors during radiotherapy, but blood — a vital, circulating tissue — has largely been excluded from dose calculations. Each blood cell passing through... Read more

Immunology

view channel
Image: The VENTANA HER2 (4B5) test is now CE-IVDR approved (Photo courtesy of Roche)

Companion Diagnostic Test Identifies HER2-Ultralow Breast Cancer and Biliary Tract Cancer Patients

Breast cancer is the most common cancer in Europe, with more than 564,000 new cases and 145,000 deaths annually. Metastatic breast cancer is rising in younger populations and remains the leading cause... Read more

Technology

view channel
Image: Conceptual design of the CORAL capsule for microbial sampling in the small intestine (H. Mohammed et al., Device (2025). DOI: 10.1016/j.device.2025.100904)

Coral-Inspired Capsule Samples Hidden Bacteria from Small Intestine

The gut microbiome has been linked to conditions ranging from immune disorders to mental health, yet conventional stool tests often fail to capture bacterial populations in the small intestine.... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.