We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Serum Vimentin Predicts Mortality in Pediatric Severe Sepsis

By LabMedica International staff writers
Posted on 14 Jun 2022
Print article
Image: The Human P-Selectin ELISA Kit – serum vimentin levels were positively correlated with P-selectin in pediatric severe sepsis (Photo courtesy of MULTI SCIENCES(LIANKE) BIOTECH, CO.,LTD).
Image: The Human P-Selectin ELISA Kit – serum vimentin levels were positively correlated with P-selectin in pediatric severe sepsis (Photo courtesy of MULTI SCIENCES(LIANKE) BIOTECH, CO.,LTD).

Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection. It is a leading cause of death worldwide in the pediatric population. Vascular hyperpermeability by loss of endothelial barrier integrity is a hallmark of sepsis.

Inflammatory response during sepsis triggers endothelial barrier dysfunction, consequently resulting in not just vascular leak and edema, but even shock and organ failure. The cytoskeletal filament vimentin is inherent to the endothelial phenotype and is critical for the proper function of endothelial cells. The association between serum vimentin and the prognosis of sepsis in children remains indeterminate.

Critical Care doctors at the Shanghai Children's Hospital (Shanghai, China) conducted a prospective study of pediatric severe sepsis (PSS) cases who were admitted to the pediatric intensive care unit (PICU) from January 2018 to December 2020. A total of 108 patients with PSS with a median age of 19.5 month were enrolled.

The laboratory indexes included routine blood indexes (white blood cell, platelet, C-reactive protein, lactate dehydrogenase, lactic acid [Lac]), cytokines (interleukin [IL]-6, IL-10, tumor necrosis factor-α), immune indexes (NK%, CD4+ CD8+, CD19), biomarkers of endothelial cell injury (serum intercellular adhesion molecule, serum vascular cell adhesion molecule, P-selectin).

For the serum vimentin assay, serum was collected within six hours of PICU admission and stored at –80 °C. Serum vimentin levels were determined using enzyme-linked immunosorbent assays: Human Vimentin ELISA Kit (MultiScience, LIANKE Biotech, CO., LTD, Hangzhou, China) with the range of 0.2–60 ng/mL. ELISA was performed in duplicate, and other assays were performed in strict accordance with the manufacturers’ instructions.

The scientists reported that the hospital mortality rate was 19.44% (21/108). Comparing with healthy controls, serum vimentin levels on PICU admission were significantly higher in patients with PSS. The area under the ROC curve for vimentin to predict the hospital mortality was 0.712 (95% CI: 0.578–846) with a sensitivity of 71.4% and a specificity of 70.1%. Moreover, hospital mortality was significantly higher in patients with vimentin level over the cutoff value of 24.53 ng/mL than in patients with vimentin level below 24.53 ng/L.

Serum levels of sICAM-1, sVCAM-1 and P-selectin displayed an increased tendency in non-survivors compared with survivors. NK (%) was significantly lower in non-survivors than in survivors (2.94 versus 4.34), whereas serum Lac levels at PICU admission displayed higher tendency in non-survivors (2.6 mmol/L versus 2.1 mmol/L). According to the results of correlation analysis, serum vimentin levels were positively correlated with P-selectin and NK (%).

The authors concluded that serum vimentin level as an indicator of endothelial injury is associated with the prognosis of PSS, and serum vimentin level ≥24.53 ng/mL on PICU admission predicts high risk for hospital mortality in PSS. The study was published in the August 1 2022 issue of the International Journal of Infectious Diseases.

Related Links:
Shanghai Children's Hospital 
LIANKE Biotech 

Gold Supplier
Automated, Random Access Chemistry Analyzer
LIDA 300
Anti-HIV 1/2 ELISA Test
HIV 1/2 Antibody (3rd) Test Kit (ELISA)
Portable Molecular Workstation
iPonatic III
Rapid Procalcitonin (PCT) Test

Print article
IIR Middle East



view channel
Image: OneDraw Blood Collection Device significantly reduces obstacles for drawing blood (Photo courtesy of Drawbridge Health)

Near Pain-Free Blood Collection Technology Enables High-Quality Testing

Blood tests help doctors diagnose diseases and conditions such as cancer, diabetes, anemia, and coronary heart disease, as well as evaluate organ functionality. They can also be used to identify disease... Read more


view channel
Image: The global infectious disease IVD market is expected to hit USD 57 billion by 2030 (Photo courtesy of Pexels)

Global Infectious Disease IVD Market Dominated by Molecular Diagnostics Technology

The global infectious disease in vitro diagnostics (IVD) market stood at USD 113.7 billion in 2021 and is expected to grow at a CAGR of -7.41% from 2022 to 2030 to hit around USD 56.89 billion by 2030,... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.