We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Abbott Diagnostics- Hematology Division

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
23 Sep 2021 - 25 Sep 2021

CSF Conversion Assay Identifies Lewy Body Disease Cognitive Impairment

By LabMedica International staff writers
Posted on 08 Aug 2021
Print article
Photomicrograph shows brown-immunostained alpha-synuclein in Lewy bodies (large clumps) and Lewy neurites (thread-like structures) in the neocortical tissue of a person who died with Lewy body disease (Photo courtesy of Movalley)
Photomicrograph shows brown-immunostained alpha-synuclein in Lewy bodies (large clumps) and Lewy neurites (thread-like structures) in the neocortical tissue of a person who died with Lewy body disease (Photo courtesy of Movalley)
Lewy body dementia, also known as dementia with Lewy bodies, is the second most common type of progressive dementia after Alzheimer's disease. Protein deposits, called Lewy bodies, develop in nerve cells in the brain regions involved in thinking, memory and movement (motor control).

Real-time quaking-induced conversion (RT-QuIC) is a highly sensitive assay originally used for prion detection. The "quaking" in the name of the technique refers to the fact that samples in the RT-QuIC assay are literally subjected to shaking. This action breaks apart aggregates of protein that are then further incubated, amplifying the amount of protein to detectable levels.

Neurology Scientists at the Institute of Neurological Sciences of Bologna (Bologna, Italy) investigated whether the cerebrospinal fluid (CSF) α-synuclein (α-syn) real-time quaking-induced conversion (RT-QuIC) assay accurately identifies patients with mild cognitive impairment due to probable Lewy body disease (MCI-LB).

The team applied α-syn RT-QuIC to 289 CSF samples obtained from two independent cohorts, including 81 patients with probable MCI-LB (70.7± 6.6 years, 13.6% female, Mini-Mental State Examination (MMSE) 26.1 ± 2.4), 120 with probable MCI-AD (68.6 ± 7.4 years, 45.8% female, MMSE 25.5 ± 2.8), and 30 with unspecified MCI (65.4 ± 9.3 years, 30.0% female, MMSE 27.0 ± 3 .0). Fifty-eight individuals with no cognitive decline or evidence of neurodegenerative disease and 121 individuals lacking brain α-syn deposits at the neuropathological examination were used as controls.

The investigators reported that RT-QuIC identified MCI-LB patients against cognitively unimpaired controls with 95% sensitivity, 97% specificity, and 96% accuracy, and showed 98% specificity in neuropathological controls. The accuracy of the test for MCI-LB was consistent between the two cohorts (97.3% versus 93.7%). Thirteen percent of MCI-AD patients also had a positive test; of note, 44% of them developed one core or supportive clinical feature of dementia with Lewy bodies (DLB) at follow-up, suggesting an underlying LB co-pathology.

The scientists noted that taken together, these findings are in line with preliminary results obtained in patients with isolated REM sleep behavior disorder, pure autonomic failure and those with incidental Lewy body dementia at post-mortem examination, demonstrating that patients with Lewy body dementia harbor significant alpha-synuclein seeding activity early in the course of the disease, irrespective of clinical presentation.

The authors concluded that their findings indicated that CSF α-syn RT-QuIC is a robust biomarker for prodromal DLB and accurately identified dementia with Lewy bodies in patients at the prodromal clinical stage and demonstrated high specificity in a large cohort of individuals examined neuropathologically. The study was published on July 1, 2021 in the journal Neurology.

Related Links:

Institute of Neurological Sciences of Bologna

New
Gold Supplier
Molecular Diagnostic System
Singuway 9600 Pro
New
Silver Supplier
COVID-19 NAb Test Solution
COVID-19 NAb Test Solution
New
Clinical Chemistry Analyzer
Mispa CXL Pro
New
Silver Supplier
Automated Nucleic Acid Extractor
NE48/96

Print article

Channels

Pathology

view channel
Image: The CellSearch Circulating Tumor Cell Kit is intended for the enumeration of circulating tumor cells of epithelial origin (CD45-, EpCAM+, and cytokeratins 8, 18+, and/or 19+ and PD-L1) in whole blood (Photo courtesy of CellSearch/Menarini Silicon Biosystems)

PD-L1 Expression in Circulating Tumor Cells Investigated for NSCLC

In non-small cell lung cancer (NSCLC), analysis of programmed cell death ligand 1 (PD-L1) expression in circulating tumor cells (CTCs) is a potential alternative to overcome the problems linked to the... Read more

Industry

view channel
Illustration

Global Digital Polymerase Chain Reaction (dPCR) Market Projected to Reach Close to USD 1.15 Billion by 2028

The global digital polymerase chain reaction (dPCR) market is projected to grow at a CAGR of more than 9% from over USD 0.50 billion in 2020 to nearly USD 1.15 billion by 2028, driven primarily by rising... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.