We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




Blood Biomarker Discovered for Autism Spectrum Disorder

By LabMedica International staff writers
Posted on 11 Mar 2021
Print article
Image: The principle of SOMAscan Assay that allows for the simultaneous detection of 1,300 human proteins using SOMAmer (Slow Off-rate Modified Aptamer) protein-binding reagents (Photo courtesy of SomaLogic).
Image: The principle of SOMAscan Assay that allows for the simultaneous detection of 1,300 human proteins using SOMAmer (Slow Off-rate Modified Aptamer) protein-binding reagents (Photo courtesy of SomaLogic).
Autism spectrum disorder (ASD), a heterogeneous neurodevelopmental disorder, is characterized by deficits in social communication and social interaction, with restricted, repetitive patterns of behavior, interests, or activities. ASD impacts at least one out of every 59 children in the USA, although this is likely an underestimation.

Many blood-based biomarker candidates have been investigated, including neurotransmitters, cytokines, and markers of mitochondrial dysfunction, oxidative stress, and impaired methylation. However, given the prevalence of ASD, the use of machine learning to incorporate demographic and clinical data into the analysis could more powerfully examine disease status and symptom severity.

Medical Scientists at the Johnson Center for Child Health and Development (Austin, TX, USA) enrolled a total of 154 male pediatric subjects. The ASD group was comprised of 76 subjects with a mean age of 5.6 years ± 1.7 years. The typically developing (TD) group was comprised of 78 subjects with a mean age of 5.7 years ± 2.0 years. The ethnic breakdown was as follows: 73 White/Caucasian, 32 Hispanic/Latino, 17 African American/Black, five Asian or Pacific Islander, 23 multiple ethnicities or other, and four not reported.

A fasting blood draw was performed on ASD and TD subjects between the hours of 8–10 AM in a 3.5 mL Serum Separation Tube using standard venipuncture technique. The SOMAScan platform 1.3k was used for analysis, and assays were run (SomaLogic, Boulder, CO, USA). SOMAmer aptamer reagents consist of short single-stranded DNA sequences with ‘protein-like’ appendages that allow tight and specific binding to protein targets. More than 1,100 proteins were examined using the SomaLogic protein analysis platform. A panel of nine proteins was identified as optimal for predicting ASD using three computational methods. The scientists then evaluated the biomarker panel for quality using machine learning methods.

The team reported that five proteins were common to all three prediction models used: mitogen-activated protein kinase 14 (MAPK14), immunoglobulin D (IgD), dermatopontin (DERM), ephrin type-B receptor 2 (EPHB2), and soluble urokinase-type plasminogen activator receptor (suPAR). These five proteins were defined as core proteins. Four proteins providing additive power were combined with the five core proteins, and together, the nine proteins resulted in an AUC of 86% (sensitivity 83%; specificity 84%). These proteins have pathway significance related to a number of processes, including negative regulation of CD8+, alpha-beta T cell proliferation, immune response, neuron projection retraction, MAPK14 activity, and glutamate receptor signaling, all of which have previously been associated with ASD.

Dwight German, PhD, a Professor of Psychiatry and senior author of the study, said, “The more significantly affected the child is, the higher or lower than normal the blood biomarker is. Ideally, there will be a day when a child is identified using blood biomarkers as being at risk for developing ASD and therapies can be started immediately. That would help the child develop skills to optimize their communication and learning.”

The authors concluded that all nine proteins were significantly different in ASD compared with TD boys, and were significantly correlated with ASD severity as measured by Autism Diagnostic Observation Schedule (ADOS) total scores. Using machine learning methods, a panel of serum proteins was identified that may be useful as a blood biomarker for ASD in boys. The study was published on February 24, 2021 in the journal PLOS ONE.

Related Links:
Johnson Center for Child Health and Development
SomaLogic


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
Xylazine Immunoassay Test
Xylazine ELISA

Print article

Channels

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.