We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company


Sarstedt provides laboratory and medical equipments, and develops, manufactures and sells equipment and consumables i... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Manual Aspiration by Monovette Reduces Hemolytic Sampling

By LabMedica International staff writers
Posted on 18 Aug 2021
Print article
Image: The manual aspiration of the 4.7 mL S-Monovette Lithium-Heparin Gel tube reduces hemolytic sampling (Photo courtesy of SARSTEDT AG)
Image: The manual aspiration of the 4.7 mL S-Monovette Lithium-Heparin Gel tube reduces hemolytic sampling (Photo courtesy of SARSTEDT AG)
Hemolytic blood samples are the number one cause for specimen rejection at emergency departments (ED). Triggered by unsuitable blood sampling material or incorrect handling and a related strong vacuum force, hemolytic samples often must be retaken.

Hemolysis refers to the release of hemoglobin and other intracellular components into the surrounding plasma due to the ruptured cell membrane of erythrocytes. The root cause of hemolysis in vitro is an improper sample drawing or, more specifically, an evolving strong vacuum force.

Clinical Laboratorians at the HFR Fribourg-Hôpital Cantonal (Villars-sur-Glâne, Switzerland) conducted a head-to-head study between January and April 2019. In the first eight weeks, all specimens were collected using BD Vacutainer Lithium-Heparin Gel tubes (Vacutainer, Becton, Dickinson and Company, Franklin Lakes, NJ, USA), in the second eight weeks, blood was taken using S-Monovette Lithium-Heparin Gel tubes (SARSTEDT AG, Nümbrecht, Germany) in aspiration mode. Specimens were categorized into five classes (0–30, 31–50, 51–75, 76–100, and 101+ mg/dL of cell-free hemoglobin) and for the statistical analyses, all samples exceeding 30 mg/dL were classified as hemolytic.

All blood samples from the emergency department were evaluated using a Cobas 6000, (Roche Diagnostics, Rotkreuz, Switzerland) a state-of-the-art analyzing system, and their Hemolysis Index (HI 1 = 1 mg of free cell hemoglobin in 1 dL blood plasma) was determined. Data were collected on 4,794 blood specimens (Vacutainer: 2,634 samples, S-Monovette: 2,160 samples).

The scientists reported that overall, 11.3 % of samples were rated as hemolytic because their concentration of hemolysis exceeded 30 mg/dL. This proportion differed considerably between specimens drawn by Vacutainer (17.0 %) and S-Monovette (4.3 %), meaning that, in proportion, there were four times as many hemolytic samples when using Vacutainer. While the percentage of non-hemolytic samples (HI of 0–30 mg/dl) was substantially higher for specimens drawn by S-Monovette (95.7 %) than Vacutainer (83.0 %), the opposite was true for all HI categories above 30 mg/dl.

The authors concluded that regarding hemolysis rates, a slow manual aspiration using S-Monovette was superior to vacuum tubes with predefined filling volumes, as demonstrated in the setting of their ED, which has important practical implications. This blood sampling process could be highly beneficial, not only from a financial point of view, but also with regards to reducing unnecessary tasks and stress for nursing staff and improving patient outcome overall. The study was published on July 28, 2021 in the journal Practical Laboratory Medicine.

Related Links:
HFR Fribourg-Hôpital Cantonal
Becton, Dickinson and Company
Roche Diagnostics

Gold Supplier
Saliva Collection Kit
SpeciMAX Stabilized Saliva Collection Kit
Automatic Hematology Analyzer
Auto Clinical Chemistry Analyzer
Mispa Clinia Plus
Washer Dispenser

Print article


Clinical Chem.

view channel
Image: The analysis pipeline used to investigate associations between blood metabolites, later life brain imaging measures, and genetic risk for Alzheimer’s disease (Photo courtesy of University College London)

Lipid Measurements Show Potential as Alzheimer’s Disease Biomarkers

Brain changes accompanying ageing are varied and can include pathologies that lead to cognitive impairment, the commonest of which is Alzheimer’s disease (AD). Identifying blood-based signatures of brain... Read more

Molecular Diagnostics

view channel
Image: This image depicts a DNA molecule that is methylated on both strands on the center cytosine. DNA methylation plays an important role for epigenetic gene regulation in development and cancer (Photo courtesy of Wikimedia Commons)

Study Supports Use of Methylated DNA Biomarkers for Cancer Diagnosis and Prognosis

A recent study added weight to the theory that methylated DNA biomarkers could be used for cancer diagnosis and prognosis. Methylation is a biological process by which methyl groups are added to a DNA molecule.... Read more


view channel
Image: Standard test for multiple myeloma provides clues of a rare, more deadly type (Photo courtesy of Pexels)

Standard Blood Cancer Test Provides Clues of Rare IgD Multiple Myeloma

IgD myeloma accounts for about 1% of common blood cancer multiple myeloma and has a worse prognosis. Specific testing for IgD myeloma is available at a handful of reference labs across the US, but takes... Read more


view channel
Image: QIAGEN has acquired a majority stake in enzymes provider BLIRT S.A. (Photo courtesy of QIAGEN)

Qiagen Acquires Enzymes Provider Blirt to Strengthen Sample Technologies Business

QIAGEN N.V. (Venlo, Netherlands) has signed agreements to acquire a 96% majority ownership stake in BLIRT S.A. (Gdansk, Poland), a manufacturer of recombinant enzymes for the life science industry.... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.