We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Immune Cells in Cerebrospinal Fluid Predict Response to Immunotherapy

By LabMedica International staff writers
Posted on 22 Mar 2021
Print article
Image: The BD LSRFortessa X-20 cell analyzer (Photo courtesy of BD Biosciences)
Image: The BD LSRFortessa X-20 cell analyzer (Photo courtesy of BD Biosciences)
Immune checkpoint inhibitors including anti-PD1, anti-PD-L1, and anti-CTLA4, have shown significant clinical benefits in patients with progressive or metastatic solid tumors, including some brain metastasis. Notably, these immune-based therapies have improved outcomes for some of those suffering from lung cancer and melanoma.

Some patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However, the anatomical location of brain lesions limits access to tumor material to characterize the immune phenotype. Cerebrospinal fluid (CSF) can provide fundamental information about the genomic characteristics of brain tumors and hence be used as a relatively non-invasive liquid biopsy.

A large team of Oncologists and their colleagues at the Vall d’Hebron University Hospital, Barcelona, Spain) analyzed samples from 48 patients with brain metastasis. The scientists assessed the immune cells present in the brain metastases, and in parallel, performed immune cell profiling of the CSF. They sought to identify which cell types were present in the CSF and compare them with those obtained from the metastatic lesions. A total of ten CSF samples were analyzed.

The team loaded samples into the 10× Genomics Chromium Controller for droplet-encapsulation (Pleasanton, CA, USA). Single-cell gene expression and T cell receptor T clonotypes (TCR) were produced using the Chromium Single-Cell 5′ Library, and sequenced on a NovaSeq 6000 (Illumina, San Diego, CA, USA). Immune cell populations were determined after processing by flow cytometry using BD FACSCELESTA (immune cell characterization panel) or BD LSRFortessa cell analyzer (BD Biosciences, San Jose, CA, USA). Other methods used by the team included Immunohistochemistry, Targeted RNA profiling, and Whole exome sequencing.

The team reported that tumor immune infiltration and specifically CD8+ T cell infiltration can be discerned through the analysis of the CSF. Consistently, identical T cell receptor clonotypes were detected in brain lesions and CSF, confirming cell exchange between these compartments. The analysis of immune cells of the CSF can provide a non-invasive alternative to predict the response to ICI, as well as identify the T cell receptor clonotypes present in brain metastasis.

Holger Heyn, PhD, a Genomic Scientist and co-corresponding author of the study, said, “Single cell transcriptome sequencing provides the highest resolution for the detection and monitoring of several different diseases. The identification of clonal T-cells in both metastasis and liquid biopsy is of particular interest. We have shown that the sequencing of T-cell receptors provides a cellular barcode that can be assessed outside of the tumor. Importantly, this approach opens up new avenues for the detection of systemic disease.” The study was published on March 8, 2021 in the journal Nature Communications.

Related Links:
Vall d’Hebron University Hospital
10× Genomics
Illumina
BD Biosciences


Gold Supplier
SARS-CoV-2 Antibody Test
SARS-CoV-2 UTAB FS
New
Silver Supplier
SARS-CoV-2 RT-PCR Assay
Savvygen SARS-CoV-2 Plus
New
Gold Supplier
Liquid Handling Workstation
AdvanSure E3 SYSTEM
New
Cartridge Incubator
WS-i60 Cartridge Incubator

Print article

Channels

Pathology

view channel

Certest Offers Real-Time PCR Assays for Fast Detection of MDR Bacterial Infections

CerTest Biotec (Zaragoza, Spain) has joined the fight against multidrug resistant strains (MDR) bacterial infections by developing real-time PCR assays for the fast detection of genes or punctual mutations that confer resistance to antibiotics from both Gram-positive and Gram-negative pathogens. Resistance to antibiotics... Read more

Industry

view channel
Illustration

Thermo Fisher Launches World’s First Fully Integrated Digital PCR (dPCR) System

Thermo Fisher Scientific Inc. (Waltham, MA, USA) has launched the world’s first fully integrated digital PCR (dPCR) system designed to provide highly accurate and consistent results within 90 minutes.... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.