We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
04 May 2021 - 07 May 2021
Virtual Venue

Immune Cells in Cerebrospinal Fluid Predict Response to Immunotherapy

By LabMedica International staff writers
Posted on 22 Mar 2021
Print article
Image: The BD LSRFortessa X-20 cell analyzer (Photo courtesy of BD Biosciences)
Image: The BD LSRFortessa X-20 cell analyzer (Photo courtesy of BD Biosciences)
Immune checkpoint inhibitors including anti-PD1, anti-PD-L1, and anti-CTLA4, have shown significant clinical benefits in patients with progressive or metastatic solid tumors, including some brain metastasis. Notably, these immune-based therapies have improved outcomes for some of those suffering from lung cancer and melanoma.

Some patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However, the anatomical location of brain lesions limits access to tumor material to characterize the immune phenotype. Cerebrospinal fluid (CSF) can provide fundamental information about the genomic characteristics of brain tumors and hence be used as a relatively non-invasive liquid biopsy.

A large team of Oncologists and their colleagues at the Vall d’Hebron University Hospital, Barcelona, Spain) analyzed samples from 48 patients with brain metastasis. The scientists assessed the immune cells present in the brain metastases, and in parallel, performed immune cell profiling of the CSF. They sought to identify which cell types were present in the CSF and compare them with those obtained from the metastatic lesions. A total of ten CSF samples were analyzed.

The team loaded samples into the 10× Genomics Chromium Controller for droplet-encapsulation (Pleasanton, CA, USA). Single-cell gene expression and T cell receptor T clonotypes (TCR) were produced using the Chromium Single-Cell 5′ Library, and sequenced on a NovaSeq 6000 (Illumina, San Diego, CA, USA). Immune cell populations were determined after processing by flow cytometry using BD FACSCELESTA (immune cell characterization panel) or BD LSRFortessa cell analyzer (BD Biosciences, San Jose, CA, USA). Other methods used by the team included Immunohistochemistry, Targeted RNA profiling, and Whole exome sequencing.

The team reported that tumor immune infiltration and specifically CD8+ T cell infiltration can be discerned through the analysis of the CSF. Consistently, identical T cell receptor clonotypes were detected in brain lesions and CSF, confirming cell exchange between these compartments. The analysis of immune cells of the CSF can provide a non-invasive alternative to predict the response to ICI, as well as identify the T cell receptor clonotypes present in brain metastasis.

Holger Heyn, PhD, a Genomic Scientist and co-corresponding author of the study, said, “Single cell transcriptome sequencing provides the highest resolution for the detection and monitoring of several different diseases. The identification of clonal T-cells in both metastasis and liquid biopsy is of particular interest. We have shown that the sequencing of T-cell receptors provides a cellular barcode that can be assessed outside of the tumor. Importantly, this approach opens up new avenues for the detection of systemic disease.” The study was published on March 8, 2021 in the journal Nature Communications.

Related Links:
Vall d’Hebron University Hospital
10× Genomics
Illumina
BD Biosciences


New
Gold Supplier
Tumor Marker Control
Tumor Marker Control
New
Preterm Delivery Test (phIGFBP-1)
Actim Partus 1ngeni
New
Gold Supplier
SARS-CoV-2 Antibody Test
SARS-CoV-2 UTAB FS
New
Gold Supplier
SARS-CoV-2, Flu A/B, and RSV Test
SARS-CoV-2 PLUS ELITe MGB Kit

Print article
BIOHIT  Healthcare OY

Channels

Industry

view channel
Image: Eppendorf Centrifuge 5910 Ri (Photo courtesy of Eppendorf AG)

Eppendorf Introduces Multipurpose Centrifuge 5910 Ri to Accelerate Results

Eppendorf AG (Hamburg, Germany) has introduced a new centrifuge designed to increase efficiency in the laboratory, Centrifuge 5910 Ri, a successor to the company’s popular Centrifuge 5910 R.... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.