We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
06 Jul 2020 - 10 Jul 2020
Virtual Venue
27 Jul 2020 - 30 Jul 2020

Microfluidic Device Isolates Circulating Tumor Cell Clusters

By LabMedica International staff writers
Posted on 03 Jul 2019
Print article
Image: (A and B) Photomicrographs of the layers of the device; (C) the mold ready for casting and (D) the chip mounted on a slide (Photo courtesy of San Diego State University).
Image: (A and B) Photomicrographs of the layers of the device; (C) the mold ready for casting and (D) the chip mounted on a slide (Photo courtesy of San Diego State University).
The three main challenges of cancer treatment are metastases, recurrence, and acquired therapy resistance. These challenges have been closely linked to circulating cancer cell clusters.

About 90% of cancer deaths are due to metastases, when tumors spread to other vital organs, and it has recently been realized that it's not individual cells but rather distinct clusters of cancer cells that circulate and metastasize to other organs.

A team of scientists led by San Diego State University (San Diego, CA, USA) has shown how a well-known passive micromixer design (staggered herringbone mixer - SHM) can be optimized to induce maximum chaotic advection within antibody-coated channels of dimensions appropriate for the capture of cancer cell clusters. The device’s principle design configuration is called: Single-Walled Staggered Herringbone (SWaSH).

The design of the device makes use of 32 channels, each of 200 μm width and 100 μm spacing, which will increase the available chip surface to cross-sectional area by approximately 1.4-fold. Numerous simulations were performed by varying different properties of the HB pattern, such as channel configuration, and flow velocities to optimize for our deterministic factor cell-to-surface interactions. The Cy5-labeled streptavidin was utilized to visualize the cross-linked and functionalized alginate hydrogel coating within the micro channels. Images were captured using a fluorescence Zeiss 200M microscope.

Peter Teriete, PhD, an assistant professor and co-author of the study, said, “Our device's channel design had to generate microfluidic flow characteristics suitable to facilitate cell capture via antibodies within the coated channels. So we introduced microfeatures, herringbone recesses, to produce the desired functionality. We also developed a unique alginate hydrogel coating that can be readily arrayed with antibodies or other biomolecules. By connecting bioengineering with materials science and basic cancer biology, we were able to develop a device and prove that it performs as desired.” The study was published on June 18, 2019, in the journal AIP Advances.

Related Links:
San Diego State University

Print article


Molecular Diagnostics

view channel
Image: The Chemagic 360 extracts DNA and uses transiently magnetized rods to attract the beads after binding to the nucleic acids (Photo courtesy of PerkinElmer).

Blood Group Locus Contributes to COVID-19 Severity Risk

There is considerable variation in disease behavior among patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019 (COVID-19).... Read more
Copyright © 2000-2020 Globetech Media. All rights reserved.