We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

DxGen Corp.

DxGenco is Point-Of-Care (POC) IVD company focused on developing multi-markers for people with diabetes and tuberculo... read more Featured Products: More products

Download Mobile App




New Gold and Copper Nanoshell-Enhanced Immunoblotting Technique Makes POC Diagnosis of Tuberculosis Easier

By LabMedica International staff writers
Posted on 09 Sep 2020
A team of researchers have developed a straightforward and naked-eye-sensitive nanoshell-enhanced immunoblotting technique to detect Mycobacterium tuberculosis (MTB) antigen 85B (Ag85B).

The group of researchers from DxGen Corporation (Gunpo-si, Gyeonggi-do, Korea), Chung-Ang University, ASAN Medical Center, and Choongnam National University Medical School has developed a novel gold binding peptide (GBP) that specifically binds to Ag85B secreted from MTB. More...
Also, a new Cu or Gold ion treatment method was proposed to intensify the colorimetric signal of gold nanoparticles (AuNP). Research has revealed that the economical paper-based colorimetric detection method can easily diagnose active TB with only a small amount of urine sample with high sensitivity.

The process involves sequentially dropping a urine sample containing the secretion of MTB and the AuNP-GBP conjugate onto a piece of nitrocellulose membrane paper. After that, a solution containing Cu or Gold ions is dropped on the membrane that combines with AuNPs to form a new ‘shell-shape’ nanostructure by changing the size and shape. This results in enhancing the colorimetric signal to make it strong enough to easily be detected by the naked eye. Since the developed immunoassay directly detects the antigen, it can significantly reduce false positives caused by latent TB or a past infection. Furthermore, Cu- or Gold ion treatment intensifies the colorimetric signal by 20 times, making it stronger to detect very low concentrations even with a smartphone or optical analyzer (0.93 ng/ml and 0.21 ng/ml for Cu- or Gold ion treatment, respectively).

A clinical study conducted for 52 urine samples composed of active TB, latent TB, NTM and non-infected subjects showed that the infected samples had either Ag85B or CFP10 antigens. The results demonstrated that the developed assay has a sensitivity and specificity of 71.4% and 86.7%, respectively.

"The biggest challenge in detecting POCT TB is to generate signals strong enough to be detected by inexpensive systems. By using the technology we developed, qualitative detection is possible with the naked eye, so that it can be used in low-resource countries," said Dr. Prof. Tae-Jung Park from the Chungang University, the corresponding author of the published paper.

"To implement a cost-effective product to help ending TB in various countries, we will commercialize the research results by implementing on the Epithod(R) analyzer and test kit platform," said Dr. Jinwoo Lee, CEO of DxGen Corp.



New
Gold Member
Immunochromatographic Assay
CRYPTO Cassette
Serological Pipet Controller
PIPETBOY GENIUS
New
Gold Member
Hematology Analyzer
Medonic M32B
New
Gold Member
Cardiovascular Risk Test
Metabolic Syndrome Array I & II
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Molecular Diagnostics

view channel
Image: The VeraBIND Tau assay is a novel blood test that identifies the presence of active tau pathology (Photo courtesy of 123RF)

First Blood-Based Test Measures Key Alzheimer's Biomarker in Asymptomatic and Symptomatic Individuals

Alzheimer’s disease (AD), the sixth leading cause of death in the United States, affects an estimated 7.2 million Americans aged 65 or older. Current diagnostic methods for AD are often invasive, expensive,... Read more

Hematology

view channel
Image: The microfluidic device for passive separation of platelet-rich plasma from whole blood (Photo courtesy of University of the Basque Country)

Portable and Disposable Device Obtains Platelet-Rich Plasma Without Complex Equipment

Platelet-rich plasma (PRP) plays a crucial role in regenerative medicine due to its ability to accelerate healing and repair tissue. However, obtaining PRP traditionally requires expensive centrifugation... Read more

Pathology

view channel
Image: Researchers have developed a novel method to analyze tumor growth rates (Photo courtesy of Adobe Stock)

Novel Method To Analyze Tumor Growth Rates Helps Tracks Progression Between Diagnosis and Surgery

Patients diagnosed with breast cancer often worry about how quickly their tumors grow while they wait for surgery, and whether delays in treatment might allow the disease to spread beyond the point of cure.... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.