We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

HORIBA Medical

HORIBA ABX designs, develops and distributes worldwide, in vitro diagnostic systems for biological analysis in medica... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
05 Sep 2020 - 09 Sep 2020
Virtual Venue

Human CRP-Levels Compared Using Point-of-Care Methods

By LabMedica International staff writers
Posted on 08 Oct 2019
Print article
Image: The ABX Micros CRP 200 hematology analyzer (Photo courtesy of Horiba Medical).
Image: The ABX Micros CRP 200 hematology analyzer (Photo courtesy of Horiba Medical).
Due to the rapid increase of the C-reactive protein (CRP) concentration in the event of an inflammatory disease, CRP is regarded as one of the most important acute phase proteins. Inflammation and CRP play an important role in operations, trauma, mental stress, myocardial infarction and neoplastic diseases.

The use of point-of-care (POC) methods and the measurements of CRP as a diagnostic marker have both increased over the past years. This has led to an increase in POC-methods analyzing CRP. High CRP levels are often seen as an indication for the proscribing of antibiotics. The quality of POC-systems compared to routine diagnostic measurements for the analysis of CRP is thereby of major importance, since many small practices will use POC-methods.

Biomedical scientists from the Salzburg University of Applied Sciences (Puch bei Hallein, Austria) compared POC and classical clinical chemistry methods, using 199 anonymized patient samples (104 male and 95 female patients aged from 31 to 96 years). The blood samples were selected based on a CRP concentration of more than 100 mg/L as measured by the Architect ci8200SR.

Comparisons were then made with ABX Micros CRP 200 (Horiba, Kyoto, Japan) and the i-CHROMA system. The CRP-levels on the Architect ci8200 were detected using serum samples and the method is based on a turbidimetric assay. EDTA-whole blood was used for the test procedure with the i-CHROMA and ABX. The ABX also works with a turbidimetric assay system and the i-CHROMA is based on a fluorescence sandwich immunoassay. The values from the i-CHROMA were used corrected for the hematocrit values.

The scientists reported that the results of the Architect ci8200 and the ABX showed significant differences when compared to the results of the i-CHROMA with hematocrit corrections. In addition, the values of the Architect ci8200 and the ABXs were also significantly different. Nevertheless, the most pronounced differences were seen when comparing the i-CHROMA with the Architect ci8200 with detected differences of up to 147.6 mg/L. Comparing the values of the i-CHROMA with the ABX resulted in differences of up to 92.7 mg/L. The lowest maximal difference of 39.4 mg/L is seen when comparing the results of the Architect ci8200 and the ABX.

The authors concluded that the measured CRP concentrations of the i-CHROMA strongly differ in the higher CRP concentration range when compared to the measurements performed on routine diagnostic equipment. The significantly reduced CRP measurements can strongly affect patient safety and result in wrong diagnostic measures. The study was published on September 19, 2019, in the journal Practical Laboratory Medicine.

Print article


Molecular Diagnostics

view channel
Abnormal accumulation of tau protein in neuronal cell bodies (arrow) and neuronal extensions (arrowhead) in the neocortex of a patient who had died with Alzheimer\'s disease (Photo courtesy of Wikimedia Commons)

Phosphorylated-Tau Blood Test Identifies Asymptomatic Alzheimer’s Disease

A blood test that determines circulating levels of the Alzheimer's disease (AD) biomarker protein phospho-tau217 (p-tau217) was found to distinguish AD from other neurodegenerative diseases, with significantly... Read more

Industry News

view channel
Image: ChemWell RPR Analyzer (Photo courtesy of Awareness Technology, Inc.)

Awareness Technology Announces ChemWell RPR Analyzer - The Most Significant Innovation in Syphilis Testing in Last 30 Years

Awareness Technology, Inc. (Palm City, FL, USA) has announced the most significant innovation in syphilis testing in the last 30 years with the launch of the ChemWell RPR automated nontreponemal analyzer... Read more
Copyright © 2000-2020 Globetech Media. All rights reserved.