We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
GLOBETECH PUBLISHING LLC

Fluidigm

Fluidigm Corporation focuses on the most pressing needs in translational and clinical research, including cancer, imm... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

CRISPR-based Assay Platform Detects Multiple Viruses and COVID-19 Variants

By LabMedica International staff writers
Posted on 17 Feb 2022
Print article
Image: SARS-CoV-2 RNA strand (Photo courtesy of 123rf.com)
Image: SARS-CoV-2 RNA strand (Photo courtesy of 123rf.com)

A cost-effective virus and variant detection platform has been developed that can test for up to 21 viruses, including SARS-CoV-2, other coronaviruses, and both influenza strains.

Investigators at Broad Institute of MIT and Harvard University (Cambridge, MA, USA) and Princeton University (Princeton, NJ, USA) have described the development of the diagnositic platform known as mCARMEN (microfluidic Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids). The mCARMEN platform is an upgraded and refined version of their CARMEN system, which depended on nanoliter droplets containing CRISPR/Cas 13-based nucleic acid detection reagents.

Recent computational efforts to identify new CRISPR systems uncovered a novel type of RNA targeting enzyme, Cas13. The diverse Cas13 family contains at least four known subtypes, including Cas13a (formerly C2c2), Cas13b, Cas13c, and Cas13d. Cas13a was shown to bind and cleave RNA, protecting bacteria from RNA phages and serving as a powerful platform for RNA manipulation. It was suggested that Cas13a could function as part of a versatile, RNA-guided RNA-targeting CRISPR/Cas system holding great potential for precise, robust, and scalable RNA-guided RNA-targeting applications.

The original CARMEN platform required custom equipment, involved a manually intensive eight to 10-hour workflow, and offered throughput that was too low for the requirements of a pandemic. Therefore, the investigators modified the CARMEN procedure to work on the Fluidigm (San Francisco, CA, USA) microfluidics and instrumentation platform, making it easier to run and cutting the run time in half. The investigators also streamlined the workflow for greater sensitivity, so that it could detect pathogens in samples with less genetic material. Furthermore, by using CRISPR-based enzymes Cas12 and Cas13 in combination, mCARMEN could not only detect the presence of a virus, but also measured the amount of virus in a sample.

To complement the mCARMEN protocol, the investigators developed a respiratory virus panel (RVP) to test for up to 21 viruses, including SARS-CoV-2, other coronaviruses and both influenza strains, and demonstrated its diagnostic-grade performance on 525 patient specimens in an academic setting and 166 specimens in a clinical setting. They further developed an mCARMEN panel to enable identification of six SARS-CoV-2 variant lineages, including Delta and Omicron, and evaluated it on 2,088 patient specimens. Finally, they implemented a combined Cas13 and Cas12 approach that enabled quantitative measurement of SARS-CoV-2 and influenza A viral copies in samples.

"The COVID-19 pandemic shows us that we need more testing, more often, particularly early on in a pandemic," said senior author Dr. Cameron Myhrvold, assistant professor of molecular biology at Princeton University. "COVID-19 shows us that challenging viruses will keep emerging, so we have to keep looking for them and come up with better ways of doing that."

The mCARMEN method was described in the February 7, 2022, online edition of the journal Nature Medicine.

Related Links:
Broad Institute of MIT and Harvard University 
Princeton University
Fluidigm 

 

Gold Supplier
Automated, Random Access Chemistry Analyzer
LIDA 300
New
Gold Supplier
Group A Streptococcus Antigen Test
OSOM Strep A Test
New
M. Tuberculosis Indirect Test
LIAISON QuantiFERON-TB Gold Plus
New
Microtome
M-250

Print article

Channels

Microbiology

view channel
Image: Trichinella sp. found in muscle tissue (Photo courtesy of McGill University)

Laboratory Features of Trichinellosis and Eosinophilia Threshold Determined

Trichinella nativa is a nematode worm, one of the species of the genus Trichinella, found in arctic and subarctic regions. It is highly pathogenic and has a high resistance to freezing. It is encapsulated,... Read more

Industry

view channel
Image: With Cell IDx’s acquisition, Leica Biosystems will be moving its multiplexing menu forward (Photo courtesy of Leica Biosystems)

Leica Biosystems Acquires Cell IDx, Expanding Offerings in Multiplexed Tissue Profiling

Leica Biosystems, a technology leader in automated staining and brightfield and fluorescent imaging (Nussloch, Germany), has acquired Cell IDx, Inc. (San Diego, CA, USA), which provides multiplex staining... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.