We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

EUROIMMUN AG

EUROIMMUN is an international provider of medical laboratory products for autoimmune, infection, allergy and molecula... read more Featured Products: More products

Download Mobile App




ELISAs Reliably Detect Anti-CCHFV Antibodies in Acute Infections

By LabMedica International staff writers
Posted on 19 Jan 2023
Print article
The EUROIMMUN Anti-CCHFV IgM and IgG ELISAs are based on recombinant viral nucleoprotein (Photo courtesy of EUROIMMUN)
The EUROIMMUN Anti-CCHFV IgM and IgG ELISAs are based on recombinant viral nucleoprotein (Photo courtesy of EUROIMMUN)

Crimean-Congo haemorrhagic fever (CCHF) is a life-threatening tick-borne viral disease included in the WHO priorities for research and product development for early diagnostics. EUROIMMUN (Lübeck, Germany), a PerkinElmer, Inc. (Waltham, MA; USA) company, has developed and subsequently evaluated ELISAs for the detection of specific IgM and IgG antibodies against the causative virus (CCHFV).

The EUROIMMUN Anti-CCHFV IgM and IgG ELISAs are based on recombinant viral nucleoprotein with the N-protein being the major target of the immune response. N-specific IgM is detectable after a median of 2-3 days after the onset of symptoms, followed by IgG 1-2 days later. The diagnostic performance of the novel commercial ELISAs was evaluated against established CE-marked tests. These were the EUROIMMUN CCHFV Mosaic 2 IgM and IgG indirect immunofluorescence assays (IFA), which served as reference tests, and IgM and IgG ELISAs from another manufacturer.

The EUROIMMUN Anti-CCHFV IgM and IgG ELISAs both yielded a higher sensitivity (IgM 98.0%, IgG 47.1%) than the other commercial ELISAs (IgM 95.9%, IgG 35.3%), as determined in 49 serum samples from patients with acute-phase CCHFV infection. The lower sensitivity of both IgG ELISAs compared to IFA could be attributed to varying antigen presentation between the two techniques and the study panel of acute-phase samples. However, the main value of IgG analysis is in disease monitoring and epidemiological surveillance. The EUROIMMUN IgG ELISA had previously demonstrated a sensitivity of 98.8% in patients with subsided CCHF.

The specificity of the EUROIMMUN IgM ELISA was slightly higher (86.4%) than that of the other ELISA (84.7%), as evaluated in 89 sera from control patients, healthy blood donors and patients with hantavirus or sandfly fever infections. For IgG, both assays demonstrated a specificity of 100%. The authors have summarized that the ELISAs from the two manufacturers showed a substantial qualitative agreement and a very strong positive correlation of quantitative results for both IgM and IgG. They concluded that the EUROIMMUN Anti-CCHFV ELISAs are standardised and easy-to-use tools that reliably support identification of acute CCHF cases.

The study was performed in a population in Türkiye, which has the highest number of laboratory-confirmed CCHF cases in the world. The endemic region of CCHFV encompasses countries in Africa, the Balkans, the Middle East and Asia that lie within the geographical habitat of the principal tick vector, namely ticks of the genus Hyalomma. Due to the non-availability of CCHF vaccine, the management of local outbreaks is dependent upon rapid identification of cases and appropriate infection control measures. Since ELISAs are less complex as compared to other serological methods, they are highly suitable for use in on-site laboratories involved in outbreak assistance.

Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV
Antipsychotic TDM AssaysSaladax Antipsychotic Assays
New
Urine Strips
11 Parameter Urine Strips
New
Hepatitis B Virus Test
HBs Ab – ELISA

Print article

Channels

Clinical Chemistry

view channel
Image: Rapid and non-invasive analysis of paracetamol overdose using paper arrow-mass spectrometry (Photo courtesy of Dr Simon Maher/University of Liverpool)

New Saliva Test Rapidly Identifies Paracetamol Overdose

Paracetamol is the most widely used medication worldwide, and its easy availability contributes to its frequent misuse and overdose. Overdosing on paracetamol can lead to liver toxicity, requiring hospitalization.... Read more

Hematology

view channel
Image: RHD screening just got easier with single exon NIPT testing (Photo courtesy of Devyser)

Non-Invasive Test Solution Determines Fetal RhD Status from Maternal Plasma

RhD (rhesus D) is a blood group type that can trigger immune responses. Individuals who lack RhD on their red blood cells are classified as RhD-negative. These individuals may produce antibodies against... Read more

Immunology

view channel
Image: Concept for the device. Memory B cells able to bind influenza virus remain stuck to channels despite shear forces (Photo courtesy of Steven George/UC Davis)

Microfluidic Chip-Based Device to Measure Viral Immunity

Each winter, a new variant of influenza emerges, posing a challenge for immunity. People who have previously been infected or vaccinated against the flu may have some level of protection, but how well... Read more

Microbiology

view channel
Image: A new test finds bacteria in liquids and indicate their presence by changing color (Photo courtesy of Georgia Kirkos/McMaster University)

New Hands-Free Rapid Test Detects Bacteria in Fluids

Bacteriophages, the most abundant form of life on Earth, are specialized to target and destroy specific types of bacteria. Their natural ability to fight bacteria has long been harnessed to treat infections.... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.