We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Kidney Disease Patient Urine Contains Diverse Bacteria

By LabMedica International staff writers
Posted on 15 May 2018
Print article
Image: The MiSeq bench-top sequencer (Photo courtesy of Illumina).
Image: The MiSeq bench-top sequencer (Photo courtesy of Illumina).
Bacterial communities in the bladders of men and women without clinical urinary tract infections (UTI) have been discovered and emerging studies suggests that the urinary microbiome may influence bladder health.

Chronic kidney disease (CKD) is a disease generally associated with advanced age and comorbidities, such as diabetes and obesity. The older age of adults with CKD along with accompanying comorbidities may influence midstream urine microbiome diversity and could influence urinary symptoms and bladder health.

Scientists at Loyola University Chicago (Maywood, IL, USA) and their colleagues examined the urine of 41 women and 36 men who had Stage 3 to Stage 5 kidney disease but were not on dialysis. Patients were age 60 or older and had less than 60% of kidney function, with an average of 27% of function. Sixty-nine percent of the men and 70% of the women also had diabetes, 42% of the men and 51% of the women had urinary urgency and 78% of the men and 51% of the women had nocturia.

Midstream voided urine specimens were collected using the clean-catch method. The bacterial composition was determined by sequencing the hypervariable (V4) region of the bacterial 16S ribosomal RNA gene. Genomic DNA was extracted from midstream voided urine samples using a validated mixture of lysozyme and mutanolysin. The amount of DNA in each sample was quantified using the Qubit 2.0 Flurometer. The processed samples were sequenced on the Illumina MiSeq bench-top sequencer rendering 250 base-pair paired-end reads.

The study found 19 types of bacteria in the urine samples, and few samples were overwhelmingly dominated by a single genus. In addition to Staphylococcus and Streptococcus, bacteria found in kidney patients' urine included Corynebacterium, Lactobacillus, Gardnerella, Prevotella, Escherichia¬/Shigella and Enterobacteriaceae. Even samples that contained one dominant type of bacteria also contained several other prominent types. Significant differences in diversity measures were observed for those reporting urgency urinary incontinence (UUI) versus those without UUI.

The authors concluded that that the midstream urine microbiomes are diverse in adults with CKD and that this diversity appears to be lower with more advanced CKD. The study was published on April 12, 2018, in the journal International Urology and Nephrology.

Related Links:
Loyola University Chicago

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
HLX
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
New
Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.