We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
Sekisui Diagnostics

EUROIMMUN AG

Euroimmun (part of Revvity) is an international provider of medical laboratory products for autoimmune, infection, al... read more Featured Products: More products

Download Mobile App




Commercially Available Zika Virus Immunoassays Evaluated

By LabMedica International staff writers
Posted on 01 Sep 2017
In 2015, the first cases of Zika virus infection were confirmed in Brazil, which indicated the beginning of the largest outbreak recorded with autochthonous vector-borne transmission documented in many countries across the Americas.

Several laboratories and commercial vendors have developed and evaluated molecular assays for rapid identification of Zika virus RNA, and, in some instances, other clinically relevant arboviruses, such as dengue virus (DENV) and chikungunya virus, in acute-phase clinical specimens.

Scientists led by those at the Public Health Agency of Canada (Winnipeg, MB, Canada) obtained samples from Canadian travelers who visited areas with known Zika virus transmission and consulted their physicians after symptoms consistent with Zika virus infection developed upon return. More...
They de-identified samples from 75 patients. Thirty samples were from patients with serologically confirmed Zika virus infections; 10 from patients with confirmed Zika virus infections identified by 2-target real-time reverse transcription polymerase chain reaction (RT-PCR); 10 from patients with suspected Zika virus infections, which were subsequently identified as DENV infections; and 25 acute-phase samples from flavivirus-negative persons tested by Zika virus RT-PCR and immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MAC-ELISA).

The team evaluated five Zika virus immunoassays: a conventional IgM ELISA and three MAC-ELISAs: Zika Virus Detect; anti-Zika virus IgM ELISA kit; and NovaLisa Zika Virus IgM µ-capture. On the basis of preliminary testing, they also tested the Euroimmun IgM ELISA in parallel with the Euroimmun conventional Zika virus IgG ELISA.

The assays generally showed reproducible results during independent evaluations, although specificity and sensitivity of each varied. The Euroimmun IgM and IgG ELISAs and the Abcam IgM ELISA showed a specificity of 100% for negative specimens with similar results of greater than 90% for confirmed DENV-positive samples. The NovaTec ELISA showed a specificity of 66% for negative specimens and 70% for DENV-positive specimens. Although the InBios Zika Detect ELISA showed similar specificity results for flavivirus-seronegative specimens, it showed decreased specificity for DENV-positive samples. This assay incorrectly identified 40% of these samples as Zika virus IgM positive and 40% as possible Zika virus positive.

The authors concluded that when performed in combination, the Euroimmun Zika Virus IgM and IgG ELISAs provide improved sensitivity. On the basis of their findings, the InBios Zika Virus Detect MAC-ELISA provides diagnostic results comparable to the CDC-based in-house MAC-ELISA for specimens collected from patients with primary flavivirus exposures (i.e., no detectable background immunity to DENV). The study was published in the September 2017 issue of the journal Emerging Infectious Diseases.


Platinum Member
Automated Coagulation Analyzer
Hemolumi H6
Gold Member
Neonatal Heel Incision Device
Tenderfoot
New
Fully Automated Urinalysis System
DxU 1800 Fully Automated Urinalysis System
Thyroid Test
Anti-Thyroid EIA Test
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Clinical Chemistry

view channel
Image Credit: 123RF

Screening After Pneumococcal Disease May Reveal Undiagnosed Blood Cancer or Immune Disorders

Severe pneumococcal disease requiring hospitalization often presents as pneumonia, particularly in older adults and people with cancer or compromised immune function. Because M protein testing and antibody... Read more

Technology

view channel
Image: The laser-based photoacoustic spectroscopy setup consists of a Mid-IR laser equipped with three QCL modules covering wavelengths from 5.6 μm to 12.9 μm, two silver coated mirrors (SCM), a dichroic mirror (DM) with a transmittance of 90%, a thermal power sensor head (PM) to monitor the output laser power, a mechanical chopper (MC) for frequency modulation and a CEPAS-detector with a self-designed swab holder (SH). (Credit: Graunke, T., Scholz, T., Pieniak, M. et al. Scientific Reports (2026). https://doi.org/10.1038/s41598-026-68298-9)

Laser-Based Swab Analysis Shows Promise for Detecting Disease-Linked Odor Patterns

Disease-related changes in volatile organic compounds can alter body odor, producing measurable patterns in exhaled breath and bodily fluids. Current analytical methods can be complex, time-consuming,... Read more

Industry

view channel
Image

Collaboration Combines AI Cognitive Assessment and RNA Blood Testing for Earlier Alzheimer’s Detection

Alzheimer’s disease is often identified only after substantial neurodegeneration, partly because current diagnostic pathways are fragmented and difficult to scale. As treatment shifts toward earlier intervention,... Read more
Copyright © 2000-2026 Globetech Media. All rights reserved.