We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us

Download Mobile App

Bacterial Infection Identified as Cause of Overactive Bladder Syndrome

By LabMedica International staff writers
Posted on 28 Jul 2016
Print article
Image: The DM4000 B automated LED microscope system (Photo courtesy of Leica Microsystems).
Image: The DM4000 B automated LED microscope system (Photo courtesy of Leica Microsystems).
Overactive Bladder (OAB) is currently characterized by symptoms of urgency, with or without urgency incontinence, with increased frequency, and nocturia, and in some cases pain, in the absence of urinary tract infection (UTI) or other defined underlying pathology.

The exclusion of infection is determined by failure to isolate more than 105 colony forming units (CFU)/mL of a single species of bacteria from culture of a midstream urine (MSU) specimen and negative leukocyte esterase and/or nitrate urinalysis by dipstick.

Scientists at the University of Kent (Chatham, UK) obtained bladder biopsies from local hospitals, and a catheter specimen of urine (CSU) was obtained from female patients and a MSU from male patients and non-OAB controls. All urine samples obtained were sent for conventional urinalysis and bacteria culture at the time of collection, if a UTI was subsequently identified the sample was retrospectively removed from the study.

The bladder biopsies were sectioned and stained and the morphological characteristics of the tissue evaluated. A DM4000B upright light microscope (Leica, Wetlar, Germany) was used to image the sections. A Luciferin Luciferase Adenosine triphosphate (ATP) Bioluminiscence Assay Kit was used to quantify ATP release from intact, live, bladder urothelium. ATP-evoked luminescence was quantified using a Synergy 2 luminometer (BioTek, Winooski, USA). The scientists also performed biopsy vesicle staining, biopsy ribonucleic acid (RNA) extraction and polymerase chain reactions, urine sediment immunofluorescence and urine nucleotide and nucleoside quantification.

The team found that some OAB patients had a low-grade inflammation, which is missed by conventional tests. This low-grade inflammation may ultimately result in increased sensory nerve excitation and the symptoms of OAB. They also found that in these patients the low-grade inflammation is associated with bacteria living inside the bladder wall. This was an observational study which means that no conclusions can be drawn about cause and effect. However, the findings may prompt the clinical re-classification of OAB and inform future therapeutic strategies. These might include protracted treatment with antibiotics to alleviate the symptoms of OAB in some individuals. The study was published on June 29, 2016, in the American Journal of Physiology.

Related Links:
University of Kent
Platinum Member
Flu SARS-CoV-2 Combo Test
OSOM® Flu SARS-CoV-2 Combo Test
Magnetic Bead Separation Modules
POCT Fluorescent Immunoassay Analyzer
Gold Member
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay

Print article


Clinical Chemistry

view channel
Image: PhD student and first author Tarek Eissa has analyzed thousands of molecular fingerprints (Photo courtesy of Thorsten Naeser / MPQ / Attoworld)

Screening Tool Detects Multiple Health Conditions from Single Blood Drop

Infrared spectroscopy, a method using infrared light to study the molecular composition of substances, has been a foundational tool in chemistry for decades, functioning similarly to a molecular fingerprinting... Read more

Molecular Diagnostics

view channel
Image: Protein ‘signatures’ obtained via a blood sample can be used to predict the onset of 67 diseases (Photo courtesy of Queen Mary University of London)

Protein Signatures in Blood Can Predict Risk of Developing More Than 60 Diseases

Measuring specific proteins to diagnose conditions like heart attacks, where troponin is tested, is a well-established clinical practice. Now, new research highlights the broader potential of protein measurements... Read more


view channel
Image: The Truvian diagnostic platform combines clinical chemistry, immunoassay and hematology testing in a single run (Photo courtesy of Truvian Health)

Automated Benchtop System to Bring Blood Testing To Anyone, Anywhere

Almost all medical decisions are dependent upon laboratory test results, which are essential for disease prevention and the management of chronic illnesses. However, routine blood testing remains limited worldwide.... Read more


view channel
Image: The Simplexa C. auris direct kit is a real-time polymerase chain reaction assay run on the LIAISON MDX instrument (Photo courtesy of Diasorin)

Novel Molecular Test to Help Prevent and Control Multi Drug-Resistant Fungal Pathogen in Healthcare Settings

Candida auris (C. auris) is a rapidly emerging multi drug-resistant fungal pathogen that is commonly found in healthcare environments, where it presents a challenge due to its ability to asymptomatically... Read more


view channel
Image: Beckman Coulter will utilize the ALZpath pTau217 antibody to detect key biomarker for Alzheimer\'s disease on its DxI 9000 immunoassay analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter Licenses Alzpath's Proprietary P-tau 217 Antibody to Develop Alzheimer's Blood Test

Cognitive assessments have traditionally been the primary method for diagnosing Alzheimer’s disease, but this approach has its limitations as symptoms become apparent only after significant brain changes... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.