We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

PERKIN ELMER CORPORATION

PerkinElmer provides detection technologies to detect and identify diseases, imaging technologies to help visualize s... read more Featured Products: More products

Download Mobile App




High-Capacity Serological Assay Developed for Celiac Disease

By LabMedica International staff writers
Posted on 02 Sep 2022
Print article
Image: The AutoDELFIA 1235 is an automatic immunoassay system (Photo courtesy of PerkinElmer Life Sciences)
Image: The AutoDELFIA 1235 is an automatic immunoassay system (Photo courtesy of PerkinElmer Life Sciences)

Celiac disease is a chronic inflammatory disorder of the small intestinal mucosa occurring in genetically predisposed individuals exposed to dietary gluten proteins of wheat (gliadin and glutenin), barley (hordein) or rye (secalin) origin.

Currently, in adult patients a diagnosis of celiac disease is usually made through a combination of serological testing and endoscopic biopsy. A common approach is to screen for serum IgA antibodies to transglutaminase 2 (anti-TG2 IgA) and IgG antibodies to deamidated gliadin peptides (anti-DGP IgG). The diagnosis is confirmed by histological examination of the small intestinal mucosa.

Medical Biochemists at the Oslo University Hospital (Oslo, Norway) and their international colleagues developed and clinically validated a high-throughput assay for serum IgA and IgG antibodies against transglutaminase-2 (TG2) and determined appropriate assay cut-offs for large-scale population screening for celiac disease. They screened 1,920 individuals from the general population.

The assay protocol was automated on the AutoDELFIA 1235 automated immunoassay platform (PerkinElmer Life Sciences, Oslo, Norway). Total serum IgA was also measured using the Alinity Immunoglobulin A (Abbott Diagnostics, Oslo, Norway) at the time of endoscopy in order to determine if individuals classified as anti-TG2 IgG-responders had lower levels of total IgA when compared to anti-TG2 IgA responders. Subjects with serum anti-TG2 concentrations above a preliminary cut-off (>0.3 mg*/L anti-TG2 IgA or >0.5 mg*/L anti-TG2 IgG) were offered endoscopic examination and biopsy. A diagnosis of celiac disease was given if villous atrophy (Marsh grade 3) was found.

The investigators reported that the assay had a limit of quantification of 0.25 mg*/L (anti-TG2 IgA) and 0.60 mg*/L (anti-TG2 IgG) with imprecision (CV) < 16% and <18% respectively. A total of 66 individuals were above the preliminary cut-off, and 56 underwent endoscopy. Of these, 26 were diagnosed with celiac disease. The team reported that 68% of subjects with anti-TG2 IgA ≥ 0.7 mg*/L or anti-TG2 IgG ≥ 1.0 mg*/L had biopsy-proven celiac disease, and utilization of these higher cut-offs identified 96% of biopsy-positive patients. At the time of endoscopy, all individuals with anti-TG2 IgA > 2.0 mg*/L had celiac disease, and this cut-off identified 88% of newly diagnosed celiac patients and 8% (2/26) of the newly diagnosed patients had primarily anti-TG2 IgG.

The authors concluded that their study described a robust, high-capacity and fully automated assay with excellent performance suitable for celiac disease screening in the general population. The study was published on August 9, 2022 in the journal Practical Laboratory Medicine.

Related Links:
Oslo University Hospital 
PerkinElmer Life Sciences 
Abbott Diagnostics 

Platinum Supplier
Xylazine Immunoassay Test
Xylazine ELISA
Gold Supplier
Automated Staining Unit
RAL Stainer
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Chikungunya & Dengue Virus Test
cobas CHIKV/DENV

Print article

Channels

Clinical Chemistry

view channel
Image: A module with eight micro-devices, complete with microfluidic channels and drive motors (Photo courtesy of U.S Department of Energy)

Highly Sensitive pH Sensor to Aid Detection of Cancers and Vector-Borne Viruses

Understanding the acidity or alkalinity of substances through pH measurement is crucial in many fields, from environmental monitoring to healthcare product safety. In many cases, these measurements must... Read more

Molecular Diagnostics

view channel
Image: The PrismRA blood test helps target best treatments for patients with rheumatoid arthritis (Photo courtesy of Scipher Medicine)

Groundbreaking Rheumatoid Arthritis Blood Test Predicts Treatment Response

Rheumatoid arthritis (RA), an autoimmune disease affecting joints and other systems in the body, impacts millions globally. Typically, the initial biologic treatment involves anti-inflammatory drugs from... Read more

Hematology

view channel
Image: The QScout hematology analyzer has received US FDA 510(k) clearance (Photo courtesy of Ad Astra Diagnostics)

First Rapid-Result Hematology Analyzer Reports Measures of Infection and Severity at POC

Sepsis, a critical medical condition that arises as an extreme response to infection, poses a significant health threat. It occurs when an infection triggers a widespread inflammatory response in the body.... Read more

Microbiology

view channel
Image: Current testing methods for antibiotic susceptibility rely on growing bacterial colonies in the presence of antibiotics (Photo courtesy of 123RF)

Rapid Antimicrobial Susceptibility Test Returns Results within 30 Minutes

In 2019, antimicrobial resistance (AMR) was responsible for the deaths of approximately 1.3 million individuals. The conventional approach for testing antimicrobial susceptibility involves cultivating... Read more

Pathology

view channel
Image: AI methods used in satellite imaging can help researchers analyze tumor images (Photo courtesy of Karolinska Institutet)

AI Approach Combines Satellite Imaging and Ecology Techniques for Analysis of Tumor Tissue

Advancements in tumor imaging technology have significantly enhanced our ability to observe the minute details of tumors, but this also brings the challenge of interpreting vast amounts of data generated... Read more

Industry

view channel
Image: The acquisition significantly expands Medix Biochemica’s portfolio of IVD raw materials (Photo courtesy of ViroStat)

Medix Biochemica Acquires US-Based ViroStat to Expand Infectious Diseases Antibody Offering

Medix Biochemica (Espoo, Finland), a supplier of critical raw materials to the in vitro diagnostics (IVD) industry, has acquired ViroStat LLC (Portland, ME, USA), a provider of infectious disease antibodies... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.