We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




IgM Antibodies Improve Risk Stratification for Antiphospholipid Syndrome

By LabMedica International staff writers
Posted on 08 Aug 2018
Print article
Image: The ACL AcuStar testing analyzer offers full automation of highly sensitive immunoassays (Photo courtesy of Instrumentation Laboratory).
Image: The ACL AcuStar testing analyzer offers full automation of highly sensitive immunoassays (Photo courtesy of Instrumentation Laboratory).
The antiphospholipid syndrome (APS) is an autoimmune hypercoagulable state and is characterized by thrombosis and/or pregnancy morbidity with the persistent presence of antiphospholipid antibodies (aPL).

Laboratory criteria include aPL detection by lupus anticoagulant (LAC), anti‐β2glycoprotein I (aβ2GPI) and anti‐cardiolipin (aCL) IgG/IgM antibodies. Although all aPL have the same value in the Sydney classification criteria, the clinical and diagnostic relevance of IgM aPL in APS has been debated and data on the issue has been inconclusive.

A team of scientists collaborating with their colleagues at Maastricht University Medical Center (Maastricht, the Netherlands) analyzed data on 1,068 patients from eight participating centers in Europe. Of the patients, 259 had APS-associated thrombosis, 204 had thrombosis but were negative for APS antibodies, 122 had obstetric APS, 33 had obstetric complications but no APS antibodies, 196 had an autoimmune disease other than APD, 194 were controls referred for APS testing but for reasons other than obstetric or thrombosis, and 60 had unspecified status.

The team used four commercially available solid phase assays; the results showed that LAC, IgG, and IgM antibodies had significant odds ratios for thrombosis and/or pregnancy morbidity, regardless of the assay used. However, in general, IgG positivity is more strongly correlated with thrombosis and pregnancy morbidity than IgM positivity. LAC positivity was independently correlated with thrombosis and/or pregnancy morbidity, isolated IgM positivity (with negative LAC results) did not have a correlation on any of the assays, while isolated IgG positivity showed a correlation on two of the four platforms.

The addition of IgM to the LAC-IgG panel did add value in thrombotic and pregnancy morbidity risk stratification, improving the odds ratio on the ACL AcuStar test for pregnancy morbidity from 2.9 to 5.1 and on the QUANTA Lite ELISA test, from 4.8 to 10.3. However, assessments of patients with thrombosis and/or pregnancy morbidity showed that IgM titers were not higher in those patients compared with controls on three of the four assays. As part of the full aPL panel, aCL and aβ2GPI IgM correlate significantly with thrombosis and/or pregnancy morbidity. The data suggest that detection of LAC and IgG aPL is sufficient for the identification of APS patients. Triple positivity for the IgG, but not IgM isotype can improve risk stratification in APS patients.

Walid Chayoua, PhD, a biochemist and a co-author of the study, said, “With the current antiphospholipid panel, aCL and anti‐β2glycoprotein I IgM antibodies do not have an added valued in the diagnosis of antiphospholipid syndrome. However, aCL and anti‐β2glycoprotein I IgM positivity are of added value in risk stratification for both thrombosis and pregnancy morbidity. All aPL included in the aPL panel are significantly correlated with thrombosis and pregnancy morbidity, independent of the isotype and solid phase assay.” The study was presented at the International Society on Thrombosis and Haemostasis (ISTH) 64th Annual Scientific and Standardization Committee (SSC) Meeting, held July 18-21, 2018, in Dublin, Ireland.

Related Links:
Maastricht University Medical Center

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
Reagent Reservoirs
Reagent Reservoirs

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.