We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Thermo Fisher Scientific

Thermo Fisher Scientific Inc. serves customers who are accelerating life sciences research, solving complex analytica... read more Featured Products: More products

Download Mobile App




Immunoassay Biomarkers Evaluated for Preeclampsia Diagnosis

By LabMedica International staff writers
Posted on 17 Aug 2017
Preeclampsia is one of the leading hypertensive disorders in pregnant women worldwide and it is estimated to cause more than 60,000 deaths per year. More...
Preeclampsia is induced by placental and maternal vascular dysfunction, which affects both maternal and neonatal health.

The current diagnosis of preeclampsia is based on non-specific tests such as new onset of hypertension and proteinuria after 20 weeks of gestation. More recently, two emerging biomarkers, anti-angiogenic factor soluble fms-like tyrosine kinase 1 (sFlt-1) and pro-angiogenic factor placental growth factor (PlGF), have been demonstrated to be involved in the pathophysiology of preeclampsia.

Scientists at the University of Chicago, Chicago, IL, USA) and their colleagues examined K2-EDTA plasma samples from 50 patients on, an automated immunoassay platform. Quality control (QC) materials were used to access intra- and inter-precision of the assay. Lower limit of quantitation and interference studies were determined using pooled patient plasma. Studies have shown that the usefulness of sFlt-1/PlGF ratio in confirming or ruling out suspected preeclampsia cases. Thus wider availability of the automated measurement of sFlt-1 and PlGF has the potential of improving the clinical management of preeclampsia.

The team used the Brahms Kryptor automated immunoassay platform (BRAHMS, Thermo Fisher Scientific, Waltham, MA, USA) and demonstrated an analytical measuring range for sFlt-1 and PlGF assays of 90-69,000 pg/mL and 11-7,000 pg/mL, respectively. The lower limit of quantitation (20% CV) was interpolated to be 35 pg/mL for sFlt-1 and 10 pg/mL for PlGF. Total precision for both assay displayed CVs of less than 10%. Interference studies showed that both assays were not significantly affected by hemolysis up to an H-index of 1100 for sFlt-1 and 300 for PlGF; L- and I- index of 800 and 80 respectively for both assays. When compared to another institute’s method the larger differences in overall bias based on individual tests however was greatly improved when a ratio of sFlt/PlGF is taken into account for method comparison.

The authors concluded that the pre-eclamptic biomarkers, sFlt-1 and PlGF assayed on the Brahms Kryptor platform, demonstrate good analytical performance and are acceptable for clinical studies defining the role of these biomarkers for preeclampsia. The study was presented at the 69th Annual meeting of American Association for Clinical Chemistry held August 1–3, 2017, in San Diego, CA, USA.


Gold Member
Troponin T QC
Troponin T Quality Control
3-Part Differential Hematology Analyzer
Swelab Alfa Plus Sampler
New
Anti-Thyroid Peroxidase Assay
LIAISON Anti-TPO
New
Silver Member
Fibrinolysis Assay
HemosIL Fibrinolysis Assay Panel
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Molecular Diagnostics

view channel
Image: The RNA-seq based diagnostic test for pediatric leukemia ensures better outcomes for children with this common cancer (Photo courtesy of Qlucore)

RNA-Seq Based Diagnostic Test Enhances Diagnostic Accuracy of Pediatric Leukemia

A new unique test is set to reshape the way Acute Lymphoblastic Leukemia (BCP-ALL) samples can be analyzed. Qlucore (Lund, Sweden) has launched the first CE-marked RNA-seq based diagnostic test for pediatric... Read more

Hematology

view channel
Image: CitoCBC is the world first cartridge-based CBC to be granted CLIA Waived status by FDA (Photo courtesy of CytoChip)

Disposable Cartridge-Based Test Delivers Rapid and Accurate CBC Results

Complete Blood Count (CBC) is one of the most commonly ordered lab tests, crucial for diagnosing diseases, monitoring therapies, and conducting routine health screenings. However, more than 90% of physician... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.