We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics


Beckman Coulter develops, manufactures and markets laboratory systems, reagents, centrifugation, lab automation, elec... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
24 Jan 2022 - 27 Jan 2022

Folate, Vitamin B12 Tied to GDM Risk in Early Pregnancy

By LabMedica International staff writers
Posted on 12 Oct 2021
Print article
Image: The SCIEX QTRAP 6500+ system combines triple quadrupole mass spectrometry with linear ion trap (LIT) (Photo courtesy of Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences)
Image: The SCIEX QTRAP 6500+ system combines triple quadrupole mass spectrometry with linear ion trap (LIT) (Photo courtesy of Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences)
Gestational diabetes mellitus (GDM) is a common medical disorder in pregnancy, estimated to affect more than 20 million pregnancies worldwide, and causes significant short- and long-term consequences to both the women and their offspring.

Folate and B12 are essential micronutrients for the metabolism of single carbon atoms (known as 1-C metabolism) and these pathways are involved in DNA methylation and synthesis of amino acids, nucleic acids and lipids. Low vitamin B12 and low/high folate levels may contribute to GDM risk, but there is conflicting evidence.

Health Scientist at the Warwick Medical School (Coventry, UK) carried out a prospective, multi-center, multi-ethnic cohort study of 4,746 participants who were pregnant women aged between 18 and 45 years who were at less than 16 weeks of gestation. A random blood sample at booking and fasting and two hour blood samples at Oral Glucose Tolerance Test (OGTT) using 75 grams of anhydrous glucose after an overnight fast of at least 10 hours were taken.

Plasma glucose was determined by the hexokinase enzymatic method using a Synchron CX7 auto-analyzer (Beckman Coulter, Fullerton, CA, USA). Serum B12 and folate were measured by electro-chemiluminescent immunoassay (Roche Cobas analyzer, Roche Diagnostics, Burgess Hill, UK). Plasma total homocysteine (tHcy) was determined by stable isotopic dilution analysis using a Shimadzu HPLC system (Milton Keynes, UK) with an auto-sampler coupled to the detection system of an AB Sciex 6500 QTrap tandem mass spectrometer (liquid chromatography mass spectrometry [LCMS]) (Applied Biosystems, Warrington, UK).

The investigators reported that GDM prevalence was between 12.5% and 14.7%. Folate deficiency (1.3%) was rare but B12 insufficiency (42.3% at <220 pmol/L) and folate excess (36.5%) were common in early pregnancy. Early pregnancy median B12 levels were lower and folate levels higher, in women who were diagnosed with GDM at 26–28 weeks and B12 was negatively associated with fasting plasma glucose. Total homocysteine was inversely associated with blood glucose level and risk of GDM. The associations strengthened when adjusted for BMI, suggesting that the total homocysteine effect on blood glucose level/GDM is independent of BMI, B12 and folate.

The authors concluded that B12 insufficiency and folate excess were common in early pregnancy. Low B12 and high folate levels in early pregnancy were associated with small but statistically significant changes in maternal blood glucose level and higher relative risk (RR) of GDM. The findings warrant additional studies on the role of unmetabolized folic acid in glucose metabolism and investigating the effect of optimizing early pregnancy or pre-conception B12 and folate levels on subsequent hyperglycemia. The study was originally published on July 22, 2021 in the journal Diabetologia.

Related Links:
Warwick Medical School
Beckman Coulter
Roche Diagnostics
Applied Biosystems

Gold Supplier
TBI Blood Test
i-STAT TBI Plasma Test
Automatic Urine Sediment Analyzer
RT-PCR Master RNA Mix
Ductless Fume Hood
Purair ECO 20

Print article
IIR Middle East


Molecular Diagnostics

view channel
Image: The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries (Photo courtesy of Takara Bio)

Cell-free RNA Profiles Gauge Preeclampsia Risk Months Before Symptoms

The period from conception to delivery represents the most rapid growth and development in an individual’s life. The ability to support this development requires dramatic and inadequately understood alterations... Read more


view channel
Image: QX200 AutoDG ddPCR System and QXDx BCR-ABL %IS Kit (Photo courtesy of Bio-Rad)

ddPCR Developed for BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia

Droplet digital polymerase chain reaction (ddPCR) is a novel polymerase chain reaction (PCR) technique reliant on massive sample partitioning to generate individual reaction chambers (droplets) in which... Read more


view channel

Global Coagulation Analyzers Market Driven by Growing Awareness About Timely Diagnosis of Blood Clots

The global coagulation analyzers market is expected to reach USD 11.7 billion by 2028, driven by the increasing understanding of blood clots and the fact that they can be avoided with early detection.... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.