We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Salivary Molecular Spectroscopy Used to Monitor Diabetes

By LabMedica International staff writers
Posted on 28 Apr 2020
Print article
Image: The Vertex 70 Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectrophotometer (Photo courtesy of the University of Guelph).
Image: The Vertex 70 Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectrophotometer (Photo courtesy of the University of Guelph).
Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia which results from insufficient secretion and/or reduced insulin action in peripheral tissues. Frequent monitoring of diabetes is essential for improved glucose control and to delay clinical complications related with diabetes.

Saliva reflects several physiological functions of the body. Salivary biomarkers might be an attractive alternative to blood for early detection, and for monitoring systemic diseases and among the advantages, saliva is simple to collect, non-invasive, convenient to store and, compared to blood, requires less handling during clinical procedures.

An international team of scientists led by those at the Federal University of Uberlandia (Uberlandia, Brazil) used a scientific system known as Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy. The team utilized the Vertex 70 ATR-FTIR spectrophotometer (Bruker Optics, Reinstetten, Germany) using a micro-attenuated total reflectance (ATR) component, to evaluate saliva of non-diabetic (ND), diabetic (D) and insulin-treated diabetic (D+I) rats to identify potential salivary biomarkers related to glucose monitoring.

The scientists reported that the spectrum of saliva of ND, D and D+I rats displayed several unique vibrational modes and from these, two vibrational modes were pre-validated as potential diagnostic biomarkers by ROC curve analysis with significant correlation with glycemia. Compared to the ND and D+I rats, classification of D rats was achieved with a sensitivity of 100%, and an average specificity of 93.3% and 100% using bands 1452 cm-1 and 836 cm-1, respectively. Moreover, 1452 cm-1 and 836 cm-1 spectral bands proved to be robust spectral biomarkers and highly correlated with glycemia.

Robinson Sabino-Silva, PhD, an adjunct professor and senior author of the study. “The present protocol used in the infrared platform is able to detect spectral biomarkers without reagents. The combination of a non-invasive salivary collection and a reagent-free analysis permit us to monitor diabetes with a sustainable platform classified as green technology.”

Matthew Baker, PhD, a Reader at Strathclyde University (Glasgow, UK) and a co-author of the study, said, “Frequent monitoring of diabetes is essential for improved glucose control and to delay clinical complications related to the condition. Early screening is also paramount in reducing these complications worldwide.”

The authors concluded that these salivary results indicate that ATR-FTIR spectroscopy coupled with univariate or multivariate chemometric analysis has the potential to provide a novel noninvasive approach to diabetes monitoring assisting medical decision making to avoid under-treatment or over-treatment with insulin. The study was published on March 17, 2020 in the journal PLOS ONE.


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
Complement 3 (C3) Test
GPP-100 C3 Kit
New
Gold Member
Cancer Mutation Profiling Liquid Kit
OncoScreen Plus

Print article

Channels

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.