We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us


Beckman Coulter develops, manufactures and markets laboratory systems, reagents, centrifugation, lab automation, elec... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Folate, Vitamin B12 Tied to GDM Risk in Early Pregnancy

By LabMedica International staff writers
Posted on 12 Oct 2021
Print article
Image: The SCIEX QTRAP 6500+ system combines triple quadrupole mass spectrometry with linear ion trap (LIT) (Photo courtesy of Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences)
Image: The SCIEX QTRAP 6500+ system combines triple quadrupole mass spectrometry with linear ion trap (LIT) (Photo courtesy of Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences)
Gestational diabetes mellitus (GDM) is a common medical disorder in pregnancy, estimated to affect more than 20 million pregnancies worldwide, and causes significant short- and long-term consequences to both the women and their offspring.

Folate and B12 are essential micronutrients for the metabolism of single carbon atoms (known as 1-C metabolism) and these pathways are involved in DNA methylation and synthesis of amino acids, nucleic acids and lipids. Low vitamin B12 and low/high folate levels may contribute to GDM risk, but there is conflicting evidence.

Health Scientist at the Warwick Medical School (Coventry, UK) carried out a prospective, multi-center, multi-ethnic cohort study of 4,746 participants who were pregnant women aged between 18 and 45 years who were at less than 16 weeks of gestation. A random blood sample at booking and fasting and two hour blood samples at Oral Glucose Tolerance Test (OGTT) using 75 grams of anhydrous glucose after an overnight fast of at least 10 hours were taken.

Plasma glucose was determined by the hexokinase enzymatic method using a Synchron CX7 auto-analyzer (Beckman Coulter, Fullerton, CA, USA). Serum B12 and folate were measured by electro-chemiluminescent immunoassay (Roche Cobas analyzer, Roche Diagnostics, Burgess Hill, UK). Plasma total homocysteine (tHcy) was determined by stable isotopic dilution analysis using a Shimadzu HPLC system (Milton Keynes, UK) with an auto-sampler coupled to the detection system of an AB Sciex 6500 QTrap tandem mass spectrometer (liquid chromatography mass spectrometry [LCMS]) (Applied Biosystems, Warrington, UK).

The investigators reported that GDM prevalence was between 12.5% and 14.7%. Folate deficiency (1.3%) was rare but B12 insufficiency (42.3% at <220 pmol/L) and folate excess (36.5%) were common in early pregnancy. Early pregnancy median B12 levels were lower and folate levels higher, in women who were diagnosed with GDM at 26–28 weeks and B12 was negatively associated with fasting plasma glucose. Total homocysteine was inversely associated with blood glucose level and risk of GDM. The associations strengthened when adjusted for BMI, suggesting that the total homocysteine effect on blood glucose level/GDM is independent of BMI, B12 and folate.

The authors concluded that B12 insufficiency and folate excess were common in early pregnancy. Low B12 and high folate levels in early pregnancy were associated with small but statistically significant changes in maternal blood glucose level and higher relative risk (RR) of GDM. The findings warrant additional studies on the role of unmetabolized folic acid in glucose metabolism and investigating the effect of optimizing early pregnancy or pre-conception B12 and folate levels on subsequent hyperglycemia. The study was originally published on July 22, 2021 in the journal Diabetologia.

Related Links:
Warwick Medical School
Beckman Coulter
Roche Diagnostics
Applied Biosystems

Gold Supplier
COVID-19 Real Time RT-PCR Kit
respiraScreen 1
Semi-Automated Coagulation Analyzer
Thrombo Fast+
Anti-SARS-CoV-2 Controls Kit
ACCURUN Anti-SARS-CoV-2 Controls Kit
Undercounter Laboratory Refrigerator

Print article


Molecular Diagnostics

view channel
Image: Absence of nuclear immunohistochemical staining of MSH2 protein (A) and presence of MLH1 protein (B) in urothelial cell carcinoma of the urinary bladder of a patient carrying a germline MSH2 mutation. Observe the nuclear staining in stromal cells as an internal control (Photo courtesy of Radboud University Nijmegen Medical Centre)

Simple Urine Test Detects Urothelial Cancers in Lynch Syndrome Patients

Lynch Syndrome (LS) is an inherited genetic disorder that carries a high risk of cancer. LS is caused by mutations affecting MLH1, MSH2, MSH6 or PMS2 genes. More than one in 300 people have LS but most... Read more


view channel
Image: The Gazelle Hb Variant Test for screening, diagnosis and management of sickle cell disease and related hemoglobinopathies at the point of care (Photo courtesy of Hemex Health)

Point-of-Care Device Accurately Rapidly Diagnoses Sickle Cell Disease

Hemoglobinopathies are the most common autosomal hereditary disorders. Approximately 7% of the global population carries hemoglobin gene mutation including structural hemoglobin variants like sickle hemoglobin... Read more


view channel
Image: The IMMULITE 2000 XPi Immunoassay System provides multiple tests on a single, easy-to-use analyzer, including the thyroid-stimulating immunoglobulin assay (Photo courtesy of Siemens Healthcare)

Immunoassays Evaluated for Thyroid-Stimulating Receptor Antibody in Graves’ Disease

Graves' disease (GD), also known as toxic diffuse goiter, is an autoimmune disease that affects the thyroid. It frequently results in and is the most common cause of hyperthyroidism and it also often results... Read more


view channel
Image: The sciREADER CL2 enables high quality digital colorimetric imaging of various support formats (Photo courtesy of SCIENION)

Multiplex Immunoassay Developed for Confirmation and Typing of HTLV Infections

Human T-Cell Lymphotropic Viruses (HTLV) type 1 and type 2 account for an estimated five to 10 million infections worldwide and are transmitted through breast feeding, sexual contacts and contaminated... Read more


view channel

AI Accurately Detects and Diagnoses Colorectal Cancer from Tissue Scans As Well or Better Than Pathologists

Artificial intelligence (A) can accurately detect and diagnose colorectal cancer from tissue scans as well or better than pathologists, according to a new study. The study, which was conducted by researchers... Read more


view channel
Image: PKeye Workflow Monitor System (Photo courtesy of PerkinElmer, Inc.)

PerkinElmer’s New Cloud-Based Platform Enables Laboratory Personnel to Remotely Manage Its Instruments in Real-Time

PerkinElmer, Inc. (Waltham, MA; USA) has launched its PKeye Workflow Monitor, a cloud-based platform enabling laboratory personnel to remotely manage and monitor their PerkinElmer instruments and workflows... Read more


view channel

Global Point of Care Diagnostics Market to Top USD 35 Billion by 2027 Due to Rising Diabetic Cases

The global point of care diagnostics market is projected to grow at a CAGR of close to 6% from more than USD 23 billion in 2020 to over USD 35 billion by 2027, driven by an increase in the number of diabetic... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.