We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Roche Diagnostics

Develops, manufactures, and markets a wide range of in vitro diagnostic systems, instruments, reagents, and tests read more Featured Products: More products

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Gene Expression Profiling of Mucinous Ovarian Tumors Identifies Prognosis Markers

By LabMedica International staff writers
Posted on 18 Oct 2022
Print article
Image: The BenchMark ULTRA system is Roche Tissue Diagnostics’ most innovative, fully-automated immunohistochemistry and in situ hybridization slide staining system (Photo courtesy of Roche Group)
Image: The BenchMark ULTRA system is Roche Tissue Diagnostics’ most innovative, fully-automated immunohistochemistry and in situ hybridization slide staining system (Photo courtesy of Roche Group)

Advanced stage mucinous ovarian tumors have poor chemotherapy response and prognosis and lack biomarkers to aid Stage I adjuvant treatment. Differentiating primary mucinous ovarian carcinoma (MOC) from gastrointestinal (GI) metastases to the ovary is also challenging due to phenotypic similarities.

MOC is a rare histological type that is less well characterized compared to more common ovarian cancer histotypes. A clinical problem frequently encountered in patients diagnosed with advanced stage MOC is the uncertainty as to whether the primary cancer is ovarian or metastatic from other sites. Metastases typically originate from the gastrointestinal (GI) tract, and the primary tumor may not be evident at surgery or on imaging.

A large international team of Medical Scientists led by the group at The University of New South Wales (Sydney, Australia) analyzed clinicopathological and gene expression data were to identify prognostic and diagnostic features. Discovery analyses selected 19 genes with prognostic/diagnostic potential. Validation was performed through the Ovarian Tumor Tissue Analysis consortium and GI cancer biobanks comprising 604 patients: 333 with MOC, 151 with mucinous borderline ovarian tumors (MBOT), 65 with upper G, and 55 with lower GI tumors.

RNA samples from a total of 634 patients were eligible for the NCounter PlexSet assay (Nanostring, Seattle, WA, USA) extracted from either 403 formalin-fixed, paraffin embedded (FFPE) whole sections, 191 FFPE cores, or 40 fresh-frozen sections. A second sample was analyzed in a subset of 33 patients: either multiple blocks from the same tumor or multiple tumor tissue sites. The team performed ERBB2/HER2 immunochemistry (IHC) using anti-HER2/neu (4B5), and Silver-enhanced in situ hybridization (SISH) using HER2/Ch17 Dual ISH DNA Probe Cocktail, (Roche Diagnostics, Indianapolis, IN, USA). Staining was performed on the Ventana Benchmark ULTRA Platform (Roche Group, Tucson, AZ, USA) on 4 µm tissue microarray sections for a subset of cases from one study.

The investigators reported that infiltrative pattern of invasion was associated with decreased overall survival (OS) within 2-years from diagnosis, compared with expansile pattern in Stage I MOC (hazard ratio HR 2.77). Increased expression of THBS2 and TAGLN were associated with shorter OS in MOC patients, (HR 1.25) and (1.21) respectively. ERBB2 (HER2)-amplification or high mRNA expression was evident in 64/243 (26%) of MOCs, but only 8/243 (3%) were also infiltrative (4/39, 10%) or Stage III/IV (4/31, 13%).

The authors concluded that an infiltrative growth pattern infers poor prognosis within 2-years from diagnosis and may help select Stage I patients for adjuvant therapy. High expression of THBS2 and TAGLN in MOC confer an adverse prognosis and is upregulated in the infiltrative subtype which warrants further investigation. Anti-HER2 therapy should be investigated in a subset of patients. MOC samples clustered with upper GI, yet markers to differentiate these entities remain elusive, suggesting similar underlying biology and shared treatment strategies. The study was published on October 12, 2022 in the journal Clinical Cancer Research.

Related Links:
The University of New South Wales
Nanostring 
Roche Diagnostics 
Roche Group

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
New
Gold Member
Magnetic Bead Separation Modules
MAG and HEATMAG

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The RedDrop One blood collection device has received 510(k) clearance from the U.S. FDA for prescription use (Photo courtesy of RedDrop Dx)

Innovative Blood Collection Device Overcomes Common Obstacles Related to Phlebotomy

The discomfort associated with traditional blood draws leads to a significant issue: approximately 30% of diagnostic tests prescribed by physicians are never completed by patients. This avoidance is often... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.