We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Molecular Diagnostic Tool Developed for Chronic Endometritis

By LabMedica International staff writers
Posted on 04 Apr 2018
Print article
Image: A histological micrograph showing endometrium with abundant plasma cells, which are diagnostic for chronic endometritis and scattered neutrophils (Photo courtesy of Nephron).
Image: A histological micrograph showing endometrium with abundant plasma cells, which are diagnostic for chronic endometritis and scattered neutrophils (Photo courtesy of Nephron).
Chronic endometritis is a persistent inflammation of the endometrial mucosa caused by bacterial pathogens. Although chronic endometritis can be asymptomatic, it is found in up to 40% of infertile patients and is responsible for repeated implantation failure and recurrent miscarriage.

Diagnosis of chronic endometritis is based on hysteroscopy of the uterine cavity, endometrial biopsy with plasma cells being identified histologically, while specific treatment is determined based on microbial culture. However, not all microorganisms implicated are easily or readily cultured needing a turnaround time of up to one week.

A team of international scientists working with those at Stanford University (Stanford, CA, USA) assessed endometrial samples from 113 patients assessed for chronic endometritis using at least one or several conventional diagnostic methods namely histology, hysteroscopy, and/or microbial culture. These samples were evaluated by reverse transcription polymerase chain reaction (RT-PCR) for the presence of nine chronic endometritis pathogens: Chlamydia trachomatis, Enterococcus, Escherichia coli, Gardnerella vaginalis, Klebsiella pneumoniae, Mycoplasma hominis, Neisseria gonorrhoeae, Staphylococcus, and Streptococcus. The sensitivity and specificity of the molecular analysis versus the classical diagnostic techniques were compared in the 65 patients assessed by all three-recognized classical methods.

The molecular method showed concordant results with histological diagnosis in 30 samples (14 double positive and 16 double negative) with a matching accuracy of 46.2%. Concordance of molecular and hysteroscopic diagnosis was observed in 38 samples (37 double positive and one double negative), with an accuracy of 58.5%. When the molecular method was compared to microbial culture, concordance was present in 37 samples (22 double positive and 15 double negative), a matching rate of 56.9%.

When cases of potential contamination and/or non-cultivable bacteria were considered, the accuracy increased to 66.2%. Of these 65 patients, only 27 patients had consistent histological plus hysteroscopic diagnosis revealing a 58.6% of non-concordant results. Only 13 out of 65 patients (20%) had consistent histology plus hysteroscopy plus microbial culture results. In these cases, the molecular microbiology matched in 10 cases showing a diagnostic accuracy of 76.9%. The molecular microbiology confirmed over half of the isolated pathogens and provided additional detection of non-culturable microorganisms.

In the endometrial samples with concordant histology plus hysteroscopy plus microbial culture results, the molecular microbiology diagnosis demonstrates 75% sensitivity, 100% specificity, 100% positive and 25% negative predictive values, 0% false positive and 25% false negative rates. The authors concluded that the molecular microbiology method is a fast, and inexpensive diagnostic tool that allows for the identification of culturable and non-culturable endometrial pathogens associated with chronic endometritis. The results obtained were similar to all three classical diagnostic methods together with a degree of concordance of 76.9% providing an opportunity to improve the clinical management of infertile patients with a risk of suffering from this ghost endometrial pathology. The study was published on February 22, 2018, in the American Journal of Obstetrics and Gynecology.

Related Links:
Stanford University

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
New
Gold Member
TORCH Panel Rapid Test
Rapid TORCH Panel Test

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.