We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




P-O-C Test Detects IgG Antibody in Serodiagnosis of Human Trichinellosis

By LabMedica International staff writers
Posted on 23 Nov 2021
Trichinellosis is one of the most critical food-borne parasitic zoonoses worldwide. More...
Humans acquire trichinellosis by ingesting raw or undercooked meats containing the infective Trichinella larvae. Adult Trichinella spp. reside in the intestinal tract of the vertebrate host; larvae can be found encapsulated in muscle tissue.

There are two classes of methods for the detection of Trichinella infection in humans or animals. Methods such as trichinoscopy (compression method) and the artificial digestion method directly test for the presence of parasites in tissues or digests. The other class of methods indirectly demonstrates infection by detecting specific antibodies using serological methods.

Medical Parasitologists at the Khon Kaen University (Khon Kaen, Thailand) and their colleagues developed an immunochromatographic test (ICT) kit to diagnose trichinellosis by detecting IgG antibodies in sera of infected humans. Human serum samples were used for the evaluation of the diagnostic properties of the test. The samples were divided into three groups: 1) the negative control group (n = 30) comprised samples from healthy adult volunteers who were free (based on stool examination using the formalin ethyl-acetate concentration technique (FECT); 2) the trichinellosis group (n = 31), which comprised samples from outbreak cases in northern Thailand) and 116 serum samples from patients with parasitic infections other than trichinellosis.

The team used somatic extract from Trichinella spiralis larvae was used as the antigen for the immunochromatographic test (ICT) kit development. Diagnostic efficacy was evaluated using human serum samples from proven trichinellosis patients, healthy persons, and those with other parasitic infections. The ICT device for diagnosing human trichinellosis was designed based on the specificity of antibodies against T. spiralis larval somatic antigens.

The investigators reported that all 31 trichinellosis serum samples yielded positive results using a cutoff value of 1. In contrast, none of the 30 healthy control sera showed positive results. Cross-reactivity was observed in serum samples from cases of gnathostomiasis (1 of 10), cysticercosis (1 of 10), paragonimiasis (3 of 10), and fascioliasis (6 of 10). The diagnostic values of the trichinellosis (Ts)-ICT were calculated with the results from all serum samples. The diagnostic sensitivity, specificity, positive and negative predictive values and accuracy of the ICT kit were 100.0%, 92.5%, 73.8%, 100.0%, and 93.8%, respectively.

The authors concluded that the ICT diagnostic kit can be used as a testing tool for human trichinellosis. This test should permit rapid detection of infection to enable prompt anthelminthic treatment. It can also be used for retrospective diagnoses and field surveys based at laboratories where sophisticated equipment is lacking. The study was published in the October, 2021 issue of International Journal of Infectious Diseases.

Related Links:
Khon Kaen University


New
Gold Member
Quality Control Material
iPLEX Pro Exome QC Panel
Serological Pipet Controller
PIPETBOY GENIUS
New
Pan-Cancer Panel
TruSight Oncology 500
New
Silver Member
Autoimmune Hepatitis Test
LKM-1-Ab ELISA
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Hematology

view channel
Image: CitoCBC is the world first cartridge-based CBC to be granted CLIA Waived status by FDA (Photo courtesy of CytoChip)

Disposable Cartridge-Based Test Delivers Rapid and Accurate CBC Results

Complete Blood Count (CBC) is one of the most commonly ordered lab tests, crucial for diagnosing diseases, monitoring therapies, and conducting routine health screenings. However, more than 90% of physician... Read more

Immunology

view channel
Image: A simple blood test could replace surgical biopsies for early detecion of heart transplant rejection (Photo courtesy of Shutterstock)

Blood Test Detects Organ Rejection in Heart Transplant Patients

Following a heart transplant, patients are required to undergo surgical biopsies so that physicians can assess the possibility of organ rejection. Rejection happens when the recipient’s immune system identifies... Read more

Pathology

view channel
Image: Pancreatic cancer diagnosis (Photo courtesy of World Journal of Gastroenterology)

AI-Driven Preliminary Testing for Pancreatic Cancer Enhances Prognosis

Pancreatic cancer poses a major global health threat due to its high mortality rate, with 467,409 deaths and 510,992 new cases reported worldwide in 2022. Often referred to as the "king" of all cancers,... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.