We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
GENRUI BIOTECH INC.

BMG LABTECH

BMG LABTECH is a manufacturer of a wide range of dedicated and multi-mode microplate instruments ranging from single-... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
04 May 2021 - 07 May 2021
Virtual Venue

Combination Urinary Biomarker Panel Detects Pancreatic Cancer Earlier

By LabMedica International staff writers
Posted on 11 Jan 2021
Print article
Image: The FLUOstar Omega Microplate Reader (Photo courtesy of BMG LABTECH).
Image: The FLUOstar Omega Microplate Reader (Photo courtesy of BMG LABTECH).
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers, with only around 9% of patients surviving more than 5 years. Asymptomatic in its initial stages, PDAC is mostly diagnosed late, when already a locally advanced or metastatic disease, as there are no useful biomarkers for detection in its early stages, when surgery can be curative.

While blood has traditionally been the main source of biomarkers, urine represents a promising alternative biological fluid. It allows a completely non-invasive sampling, high volume collection, and ease of repeated measurements; it has a lesser dynamic range, with a less complex proteome than blood.

Cancer specialist at the Queen Mary University of London (London, UK) and their colleagues obtained from multiple centers and a biomarker panel was assayed on 590 urine specimens: 183 control samples, 208 benign hepatobiliary disease samples (of which 119 were chronic pancreatitis), and 199 PDAC samples (102 stage I–II and 97 stage III–IV); 50.7% were from female individuals. PDAC samples were collected from patients before treatment. Blood and urine samples were analyzed with commercial enzyme-linked immunosorbent assay kits and the optical densities were determined using the FLUOstar Omega Microplate Reader (BMG LABTECH, Ortenberg, Germany).

The team changed their original protocol substituting Regenerating Family Member 1 Alpha (REG1A) with REG1B to enhance the performance of the panel to detect resectable PDAC. In a comparison of controls and PDAC stage I–II samples, the areas under the receiver operating characteristic curve (AUCs) increased from 0.900 and 0.926 in the training (50% of the dataset) and validation sets, respectively, to 0.936 in both the training and the validation (95% CI 0.888–0.984) datasets for the new panel including REG1B. This improved panel showed both sensitivity (SN) and specificity (SP) to be greater than 85%. Plasma CA19-9 enhanced the performance of this panel in discriminating PDAC I–II patients from controls, with AUC = 0.992, SN = 0.963, and SP = 0.967.

The authors concluded that they had successfully validated the performance of their urinary biomarker panel in detecting PDAC earlier. The PancRISK score is designed to enable further stratification of patients already predisposed to develop PDAC, thus enriching the population that should undergo follow-up, i.e., invasive clinical workup. The study was published on December 10, 2020 in the journal PLOS Medicine.





Gold Supplier
Streptococcus Test
STREPA’LERT
New
COVID-19 Antigen Test (Swab)
Rapid COVID-19 Antigen Test (Swab)
New
COVID-19 ELISA Test
Kantaro COVID-SeroKlir
New
Gold Supplier
SARS-CoV-2 Rapid Test
Vivalytic SARS-CoV-2 Rapid 39 Minute Test

Print article
BIOHIT  Healthcare OY

Channels

Molecular Diagnostics

view channel
Image: Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis (Photo courtesy of Dr. Christopher Wiley)

Novel Lipid Biomarker Detects Senescent Cells

A recent paper identified a lipid biomarker indicative of cellular senescence and described a method to evaluate its effect on the molecular events that lead to senescence. Cellular senescence is a... Read more

Industry

view channel
Image: The Luminex Aries® System (Photo courtesy of Luminex Corporation)

DiaSorin to Acquire Luminex to Broaden Positioning in Molecular Diagnostics Space

DiaSorin S.p.A. (‎Saluggia‎, Italy) has announced that its Board of Directors has unanimously approved and signed a definitive merger agreement for DiaSorin to acquire Luminex Corporation (Austin, TX,... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.