We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
SINNOWA MEDICAL SCIENCE & TECH

BIO-RAD LABORATORIES

Provides full range of instrumentation, reagent kits, software and quality control systems to clinical laboratories. ... read more Featured Products: More products

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
24 Feb 2021 - 28 Feb 2021
Virtual Venue
03 Mar 2021 - 05 Mar 2021
Virtual Venue

Cytokine Profiles Characterized in Primary Sjögren´s Syndrome Patients

By LabMedica International staff writers
Posted on 02 Dec 2020
Print article
Image: The Bio-Plex Pro Human Th17 Cytokine Panel enables measurement of analytes in diverse matrices such as serum, plasma, and cell culture supernatants (Photo courtesy of Bio-Rad).
Image: The Bio-Plex Pro Human Th17 Cytokine Panel enables measurement of analytes in diverse matrices such as serum, plasma, and cell culture supernatants (Photo courtesy of Bio-Rad).
Primary Sjögren's syndrome (pSS) is an autoimmune disease characterized by a lymphocytic infiltrate in salivary glands driving to epithelial damage. The pSS patients present heterogenic clinical and serological characteristics.

Cytokines play an essential role in pSS pathogenesis, mediating cell response and promoting tissue damage. Th1, Th2, and Th17 cells and their cytokine profiles have been implied in the pathogenesis of pSS as well as reported in saliva, serum, and labial salivary gland (LSG), with different results.

Biomedical Scientists from the Universidad de Guadalajara (Guadalajara, México) included in a study 99 pSS patients, diagnosed by the 2016 ACR/EULAR classification criteria, and 76 control subjects (CS). Ninety‐eight percent of the pSS patients had a positive biopsy of labial minor salivary glands (focus score ≥ 1 foci/4 mm2).

The team collected samples in tubes containing spray‐coated silica and a polymer gel were used for serum separation (BD Vacutainer SST, Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Cytokine quantification (IL‐1β, IL‐4, IL‐6, IL‐10, IL‐17A, IL‐17F, IL‐21, IL‐22, IL‐23, IL‐25, IL‐31, IL‐33, IFN‐γ, and TNF‐α) was performed by Bio‐Plex Pro Human Th17 Cytokine Panel 15‐Plex kit and analyzed using a Bio‐Plex MAGPIX Multiplex Reader (Bio‐Rad Laboratories, Inc, Hercules CA, USA).

The investigators reported that higher IFN‐γ, IL‐17F, IL‐21, IL‐23, IL‐4, and IL‐31 levels were observed in pSS patients in comparison with control subjects. Principal component analysis (PCA) showed three groups. The severe group was characterized by higher cytokine concentrations as well as an increase in clinical parameters such as antibody levels, damage index score, and others. The moderate group presented intermediate severity; meanwhile, the mild group presented the lowest severity. The population showed low positivity to anti‐Ro (13.3%) and anti‐La (11.1%) antibodies.

The authors concluded that cluster analysis revealed three groups that were different in cytokine levels and clinical parameters in which the mild group was defined by lower severity, the moderate group with intermediate severity, and the severe group with higher severity. This analysis could help sub-classify the primary Sjögren’s syndrome patients for a better understanding of the clinical phenotype that impacts the treatment approach. The study was published on October 17, 2020 in the Journal of Clinical Laboratory Analysis.

Related Links:
Universidad de Guadalajara
Becton, Dickinson and Company
Bio‐Rad Laboratories



Print article

Channels

Molecular Diagnostics

view channel
Image: Blood smear from a P. falciparum culture: several red blood cells have ring stages inside them while close to the center is a schizont and on the left a trophozoite (Photo courtesy of Wikimedia Commons)

Panel of MicroRNAs Differentiates Uncomplicated and Severe Malaria in Children

MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the host fluids, constitute a promising biomarker for the prognosis of severe malaria. MiRNAs comprise a class of about 20 n... Read more

Pathology

view channel
Image: The Leica Bond III stainer is fully automated Immunohistochemical and In Situ Hybridization (IHC and ISH stainer) (Photo courtesy of Leica Biosystems).

Mismatch Repair/Microsatellite Instability Evaluated Using Cytology Effusion Specimens

DNA mismatch repair (MMR) status is routinely assessed in colorectal and endometrial carcinoma as a method of cancer prevention, surveillance in patients with Lynch syndrome and their families, and for... Read more

Industry

view channel
Image: BioProfile FLEX2 Cell Culture Analyzer (Photo courtesy of Nova Biomedical)

Nova Biomedical Adds Sample Retain Collector to BioProfile FLEX2 Cell Culture Analyzer

Nova Biomedical (Waltham, MA, USA) has added a Sample Retain Collector (SRC) for its BioProfile FLEX2 automated cell culture analyzer which measures up to 16 tests including pH, gases, metabolites, osmolality,... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.