We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Diseases Diagnosed by Isolating Biomarkers in Tears

By LabMedica International staff writers
Posted on 25 Jul 2022
Print article
Image: The NanoSight NS300 can visualize and measure particles in suspension in terms of size, light scattering intensity, fluorescence and count (Photo courtesy of Mark Wainwright Analytical Centre)
Image: The NanoSight NS300 can visualize and measure particles in suspension in terms of size, light scattering intensity, fluorescence and count (Photo courtesy of Mark Wainwright Analytical Centre)

Current clinical studies pose significant challenges in distinguishing disease subtypes with accurate molecular signatures and tracking the disease progression in a non-invasive way. Identifying molecular clues in samples from patients, such as specific proteins or genes from vesicular structures called exosomes, could improve the accuracy of diagnoses.

Since small extracellular vesicles (sEVs, exosomes) reveal specific functions in various biological processes, including immune regulation, angiogenesis, tumor invasion, and cell migration, exosome-based liquid biopsy technology has offered an attractive alternative to disease classification and prognostic prediction. Tears are well-suited for sample collection because the fluid can be collected quickly and non-invasively.

Medial Scientists at the Wenzhou Medical University (Wenzhou, China) and their colleagues carried out a retrospective case-controlled study that included patients with dry eye and type 2 diabetes. A tear sample was collected by placing a Schirmer paper on each eye, and the moistened length was immediately recorded after 5 minutes of collection or at completion (length reaching at 30 mm in 5 minutes).

The tear mixture was eluted from the Schirmer strip by shaking at 4 °C for 30 minutes and two-step centrifugation (200g for 10 minutes and 3,000g for 10 minutes) to remove cells and other impurities. Exosomes were subsequently purified with the rapid-isolation system under the negative pressure of 40 kPa and conversion time of 30 seconds, respectively. Samples were loaded to the equilibrated size exclusion chromatography column (qEV original, IZON Science Ltd., Oxford, UK), and 16 sequential fractions of 0.5 mL were eluted by adding PBS. The eluted fractions 8–10 were pooled and concentrated to a final volume of 150 μL. Nanoparticle Tracking Analysis (NTA) was performed on a NanoSight NS300 system (Malvern Panalytical, Malvern, UK). Other methods included in the study were Western Blot analysis, on-device exosome detection, proteomic analysis, and exosomal miRNA sequencing.

The investigators successfully distinguished between healthy controls and patients with various types of dry eye disease based on a proteomic assessment of extracted proteins. Similarly, the Incorporated Tear Exosomes Analysis via Rapid-isolation System (iTEARS) enabled the team to observe differences in microRNAs between patients with diabetic retinopathy and those that didn't have the eye condition, suggesting that the system could help track disease progression. The team says that this work could lead to a more sensitive, faster and less invasive molecular diagnosis of various diseases, using only tears.

The authors concluded that they established the iTEARS for deciphering the biomarkers of diseases from a teardrop, which revealed the promising role of tear exosomes in disease classification and course monitoring of ocular disorders and other diseases such as neurodegenerative diseases and cancer. By being applied in extended disease types and validated with abundant clinical cases, they anticipate their teardrop-based iTEARS to be the alternative tool for point-of-care-test. The study was published on July 20, 2022 in the journal ACS Nano.

Related Links:
Wenzhou Medical University 
IZON Science Ltd 
Malvern Panalytical

New
Gold Supplier
Renin Control
Lumipulse Renin Control
New
Gold Supplier
Automated, Random Access Chemistry Analyzer
LIDA 300
New
Silver Supplier
Hemoglobin Testing System
VARIANT II TURBO
New
COL-R Assay
CHROMagar COL-APSE

Print article

Channels

Molecular Diagnostics

view channel
Image: The new multiplex QIAstat-Dx Viral Vesicular Panel is designed to support research and surveillance efforts (Photo courtesy of QIAGEN)

First Syndromic Test Differentiates Between Monkeypox and Five Pathogens with Similar Symptoms

A new syndromic test differentiates between monkeypox and five other pathogens which cause similar symptoms. This first-ever test detects the two known forms of monkeypox virus (the so-called West African... Read more

Industry

view channel
Image: The Accelerate Arc Module & BC kit is registered as an IVD (Photo courtesy of Accelerate Diagnostics)

BD and Accelerate Diagnostics Collaborate to Offer Rapid Antimicrobial Identification and Susceptibility Diagnostics

Becton, Dickinson and Company (BD, Franklin Lakes, NJ, USA) and Accelerate Diagnostics, Inc. (Tucson, AZ, USA) have entered into a worldwide commercial collaboration agreement where BD will offer Accelerate's... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.