We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Eurofins Technologies

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
06 Jun 2020 - 09 Jun 2020
Virtual Venue
08 Jun 2020 - 11 Jun 2020
Virtual Venue

Fecal Microbes Used to Diagnose Liver Cirrhosis

By LabMedica International staff writers
Posted on 09 Apr 2019
Print article
Image: The UltraClean PCR cleanup kit (Photo courtesy of MO BIO Laboratories).
Image: The UltraClean PCR cleanup kit (Photo courtesy of MO BIO Laboratories).
Nonalcoholic fatty liver disease (NAFLD) is the most prevalent cause of chronic liver disease worldwide, and yet remains largely underdiagnosed even in individuals with advanced stage of the disease.

NAFLD-cirrhosis represents the most severe stage of the disease, carries a significant risk of hepatocellular carcinoma (HCC), and is consistently identified as the most important predictor of liver-related morbidity-mortality in NAFLD.

Medical scientists at the University of California San Diego (La Jolla, CA, USA) and their associates analyzed the microbial makeup of stool samples from 98 people known to have some form of NAFLD and 105 of their first-degree relatives, including some twins. The study included 26 probands with NAFLD-cirrhosis and 37 of their first-degree relatives. At the time of each visit, patients provided stool samples. These were collected and immediately stored in a −80 °C freezer.

DNA extraction and 16S rRNA amplicon sequencing were done and DNA was extracted using the Qiagen MagAttract PowerSoil DNA kit. Amplicon PCR was performed on the V4 region of the 16S rRNA gene using the primer pair 515 f to 806r with Golay error-correcting barcodes on the reverse primer. Amplicons were barcoded and pooled in equal concentrations for sequencing. The amplicon pool was purified with the MO BIO UltraClean PCR cleanup kit and sequenced on the MiSeq sequencing platform.

The team identified 27 unique bacterial features unique to the gut microbiomes from stools of people with NAFLD-cirrhosis. They were able to use this noninvasive stool test to pick out the people with known NAFLD-cirrhosis with 92% accuracy, but more importantly, the test allowed them to differentiate the first-degree relative with previously undiagnosed NAFLD-cirrhosis with 87% accuracy. The results were confirmed by magnetic resonance imaging (MRI).

At the genus level, both NAFLD-cirrhosis and NAFLD without advanced fibrosis group were enriched with Streptococcus, but only the NAFLD-cirrhosis group was enriched with Megasphaera. Species belonging to the family Enterobacteriaceae and the genera Streptococcus and Gallibacterium were the most enriched in NAFLD-cirrhosis, while Faecalibacterium prausnitzii and species belonging to the genus, Catenibacterium and the families Rikenellaceae, Mogibacterium, Peptostreptococcaceae were enriched in non-NAFLD controls.

Rohit Loomba, MD, a professor of medicine and senior author of the study, said, “If we are better able to diagnose NAFLD-related cirrhosis, we will be better at enrolling the right types of patients in clinical trials, and ultimately will be better equipped to prevent and treat it. This latest advance toward a noninvasive stool test for NAFLD-cirrhosis may also help pave the way for other microbiome-based diagnostics and therapeutics, and better enable us to provide personalized, or precision, medicine for a number of conditions.” The study was published on March 29, 2019, in the journal Nature Communications.

Related Links:
University of California San Diego

Print article


Lab Tech.

view channel
Image: Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging (Photo courtesy of Massachusetts General Hospital Research Institute).

Ultra-Fast Cycling for Multiplexed Cellular Fluorescence Imaging

In the treatment of tumors, microenvironment plays an important role. It often contains immune cells that are so changed that they promote tumor growth. Until now, analyses of these dynamic changes with... Read more
Copyright © 2000-2020 Globetech Media. All rights reserved.