We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Assay Targets Antibiotic Resistance with Pathogen Identification

By LabMedica International staff writers
Posted on 18 Feb 2019
A rapid diagnostic device has been developed to detect and identify the source of an infection in less than one hour. More...
The proprietary DNA-based technology has the potential to revolutionize infection control by providing a POC, inexpensive, rapid diagnosis of pathogens in humans, animals and agriculture.

The C-AST assay is being developed in tandem with the CAPTURE pathogen identification assay, which stands for confirm active pathogens through an amplified RNA expression platform. The CAPTURE assay identifies bacterial or fungal pathogens' RNA in liquid samples, including blood, urine, saliva, and other types, with a "universal fluorescent marker," applying the firm's proprietary array using a machine-learning algorithm.

Earlier this month, GeneCapture scientists presented data collected from the functioning prototype system for its collaborators at the University of Alabama (Huntsville, AL, USA). They tested four antibiotics: ampicillin (AMP), amoxicillin/clavulanate (AMC), trimethoprim/sulfamethoxazole (SXT), and ciprofloxacin (CIP), against the nine pathogens responsible for most UTI cases. The group organisms include strains of species like Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus aureus.

The scientists initially evaluated 70 runs comparing 181 drug exposures to no-drug controls. They then evaluated 25 subsequent assays against culture results, focusing on AMP, AMC, SXT, and CIP, with most being tested in duplicate or triplicate. The group did not encounter false resistances or false sensitivities using the drugs. They also emphasized that the C-AST assay performed identically to culture results but in a much shorter period of time. The C-AST assay can test the effectiveness of various antibiotics against pathogens in between 5 and 75 minutes.

Peggy L. Sammon, co-founder and the CEO of GeneCapture, said, “We realized that for most infections, knowing the identity of the organism wasn't enough, so we developed the C-AST assay to give us information about the right drugs to use once CAPTURE has identified an organism. Knowing the type of organism and its concentration allows the C-AST assay to quickly analyze which antibiotics are sensitive or resistant."

Related Links:
University of Alabama


New
Gold Member
Blood Gas Analyzer
Stat Profile pHOx
3-Part Differential Hematology Analyzer
Swelab Alfa Plus Sampler
New
Automated Microscope
dIFine
New
Gold Member
Automated Cell Culture Chemistry Analyzer
BioProfile FLEX2 Basic
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Hematology

view channel
Image: CitoCBC is the world first cartridge-based CBC to be granted CLIA Waived status by FDA (Photo courtesy of CytoChip)

Disposable Cartridge-Based Test Delivers Rapid and Accurate CBC Results

Complete Blood Count (CBC) is one of the most commonly ordered lab tests, crucial for diagnosing diseases, monitoring therapies, and conducting routine health screenings. However, more than 90% of physician... Read more

Immunology

view channel
Image: A simple blood test could replace surgical biopsies for early detecion of heart transplant rejection (Photo courtesy of Shutterstock)

Blood Test Detects Organ Rejection in Heart Transplant Patients

Following a heart transplant, patients are required to undergo surgical biopsies so that physicians can assess the possibility of organ rejection. Rejection happens when the recipient’s immune system identifies... Read more

Pathology

view channel
These images illustrate how precision oncology Organ Chips recapitulate individual patients’ responses to chemotherapy (Photo courtesy of Wyss Institute at Harvard University)

Cancer Chip Accurately Predicts Patient-Specific Chemotherapy Response

Esophageal adenocarcinoma (EAC), one of the two primary types of esophageal cancer, ranks as the sixth leading cause of cancer-related deaths worldwide and currently lacks effective targeted therapies.... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.