We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Shenzhen New Industries Biomedical Engineering Co., Ltd. (SNIBE)

Shenzhen New Industries Biomedical Engineering Co., Ltd. (SNIBE) is a biotechnology company specializing in clinical ... read more Featured Products: More products

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Automated Immunoassays Evaluated for Immune Response to SARS-CoV-2

By LabMedica International staff writers
Posted on 12 Jul 2021
Print article
Image: The MAGLUMI 800 Automatic immunoassay analyzer (Photo courtesy of Snibe)
Image: The MAGLUMI 800 Automatic immunoassay analyzer (Photo courtesy of Snibe)
As part of the fight against the SARS-Cov-2 epidemic, laboratories have a critical role in assessing the reliability of new serological assays before taking part of diagnostic protocols or made available broader to the community and to evaluate commutability between assays.

Serology is a valuable tool for sero-epidemiological investigations; however, antibody detection could confirm diagnose symptomatic patients in whom the PCR was negative or not performed. Moreover, if the neutralizing capacities of the antibodies are proven, their assessment could help health care institutions to know which of their health care workers have already been in contact with the virus and if they can be mobilized if required.

Clinical microbiologists at the Université Catholique de Louvain (Brussels, Belgium) and their colleagues performed a comparison study that included four groups: 12 healthy volunteers asymptomatic whose blood had been collected during the pandemic; 17 healthy volunteers whose blood was collected before 2019; 10 samples COVID-19 free with confounding factors selected from January 2018 to August 2019, the samples included with potential cross-reaction to the SARS-CoV-2 immunoassay, such as, EBV infection, parvovirus infection, HBV infection, Bartonella henselae infection, Brucella spp. infection and various autoimmune diseases; and 44 samples of patients infected by SARS-CoV-2 confirmed by RT-PCR.

The two automated assays compared for SARS-CoV-2 IgG testing, were the MAGLUMI 800 and the LIAISON XL. The MAGLUMI 800 SARS-CoV-2 IgG (SNIBE - Shenzhen New Industries Biomedical Engineering Co., Ltd, Shenzhen, China) is a fully automated chemiluminescence immunoassay (CLIA). The LIAISON SARS-CoV-2 (Diasorin, Saluggia, Italy), uses magnetic beads coated with S1 and S2 antigens derived from the SARS-Cov-2 spike protein which is responsible for binding and fusion of the virus with the host cell membrane, these antigens are the primary target of neutralizing antibodies.

The investigators reported that in patients confirmed positive for COVID-19, the two automated assays were significantly correlated (r = 0.811). This positive correlation potentially reflects a good agreement between the two automated assays for the assessment of the intensity of antibody titer. After 10 days of symptoms onset, the concordance between the two assays was excellent. Discrepant cases between the two assays occurred at the early stage of the disease, between five and 10 days after symptom onset. The team showed that for five patients the MAGLUMI detects antibodies and the LIAISON XL analyzer did not. For these patients the test was performed between seven and 12 days after the onset of symptoms which is below the currently reported average rate of seroconversion.

The authors concluded that the overall agreement between the two automated assays for SARS-CoV-2 was good. However, the MAGLUMI assay might be more sensitive at the early stages of antibody development and there is a lack of specificity with LIAISON XL. The study was published in the August, 2021 issue of the journal Practical Laboratory Medicine.

Related Links:
Université Catholique de Louvain
Shenzhen New Industries Biomedical Engineering Co., Ltd
Diasorin


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
Xylazine Immunoassay Test
Xylazine ELISA

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.