We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
06 Feb 2023 - 09 Feb 2023

Blood Test for Myocarditis Detects Heart Inflammation

By LabMedica International staff writers
Posted on 05 Dec 2022
Print article
Image: Endomyocardial biopsy showing lymphocytic myocarditis (Photo courtesy of Marion University)
Image: Endomyocardial biopsy showing lymphocytic myocarditis (Photo courtesy of Marion University)

Myocarditis is a difficult condition to diagnose. Symptoms include a temperature, fatigue, chest pain and shortness of breath, which can all be easily mistaken for other conditions. The gold standard method for diagnosis is a heart biopsy, an expensive, invasive, and risky procedure which can sometimes still miss signs of the condition.

T cell–mediated immunity has been linked to a variety of heart diseases, from classic inflammatory cardiac conditions, such as myocarditis, to diseases without a readily evident pathogenic inflammatory component, such as hypertensive cardiomyopathy. T cell activation in cardiac inflammation often results from the interaction of an external environmental trigger (viral infection) or an endogenous stimulus (mechanical or oxidative stress) with the immune system of the host.

A large team of medical scientists led by those at Queen Mary University of London (London, UK) compared blood samples from several groups of patients, including 34 people with a final diagnosis of myocarditis. Study participants were recruited into the following patient groups: acute myocarditis (AM), idiopathic dilated cardiomyopathy (iDCM), acute ST-segment–elevation myocardial infarction (STEMI), cardiac surgery, ischemic heart failure (IHF), or active Sjögren syndrome. A group of individuals with known familial heart muscle disease (fHMD) were also studied. Healthy controls (HCs) were recruited locally. For immunohistology, postmortem tissue samples from five patients with AM and five patients with DCM were obtained. Cell proliferation was assessed using the cell proliferation modeling module on FlowJo flow cytometry software (FlowJo, Ashland, OR, USA).

The investigators looked for the presence of T-cells expressing a molecule called cMet in the blood strongly indicates that a person has myocarditis. They reported that an increased proportion of c-Met+ CD4+ memory T cells co-expressing the CXCR3 and CCR4 chemokine receptors was detectable in patients with AM or iDCM compared with the other groups. In contrast, no significant difference was detected in memory c-Met+ CD8+ CXCR3+ CCR4+T cells. However, both CD4+ and CD8+ c-Met+ CCR4+ T cells were significantly increased. A phenotypic and functional analysis of total circulating memory T cell populations of participant groups detected differences in T cell subsets and in markers of T cell activation.

The team noted that that cMet-expressing T cells levels could be detected through a routine blood test that could cost less than GBP 50 with results available within hours.

Sir Nilesh Samani, MD, a Professor of Cardiology, said, “This blood test could revolutionize the way we diagnose myocarditis, allowing doctors to step in at a much earlier stage to offer treatment and support. It would also reduce the need for the risky, invasive tests currently used, saving the NHS time and money and freeing up vital resources.”

The authors concluded that in this novel study with a limited number of patients, the potential translational implications of this work are evident, namely the diagnostic and prognostic potential of monitoring c-Met+ T cells in the venous blood, and provided a new target pathway for therapeutic intervention. The study was published on November 23, 2022 in the journal Circulation.

Related Links:
Queen Mary University of London
FlowJo 

Gold Supplier
Hematology Workflow Solution
DIFF-Line
New
POC Bacterial Infection Analyzer
Mini-lab
New
HPV Control Panel
Human Papillomavirus (HPV) Control Panel
New
Hand-Held Immunoassay & Chemistry Diagnostic System
Nanōmix eLab

Print article
MEDLAB - INFORMA

Channels

Molecular Diagnostics

view channel
Image: A novel research study moves the needle on predicting sudden cardiac arrest (Photo courtesy of Pexels)

Newly Identified Protein Biomarkers in Blood Predict Sudden Cardiac Arrest Before it Strikes

Sudden cardiac arrest, or the sudden loss of heartbeat, is a life-threatening heart condition and often fatal. Despite providing an organized emergency medical response, less than 10% of individuals having... Read more

Microbiology

view channel
Image: Medical illustration of Carbapenem-resistant Enterobacteriacea (Photo courtesy of CDC, Stephanie Rossow)

Breakthrough Test Enables Targeted Antibiotic Therapy for Various Enterobacter Species

Bacteria of the Enterobacter genus are considered to be the most dangerous bacteria linked to hospital infections across the world. Some of their representatives demonstrate high resistance to commonly-used... Read more

Technology

view channel
Image: Flexible copper sensor made cheaply from ordinary materials (Photo courtesy of University of São Paulo)

Low-Cost Portable Sensor Detects Heavy Metals in Sweat

Heavy metals like lead and cadmium can be found in batteries, cosmetics, food and many other things that have become a part of daily life. However, they become toxic if they accumulate in the human body... Read more
Copyright © 2000-2023 Globetech Media. All rights reserved.