We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Abbott Diagnostics


Boditech Med Inc. develops products for in-vitro diagnostics and diagnostic reagents. Its products include automatic ... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.
23 Jan 2021 - 27 Jan 2021
Virtual Venue
24 Feb 2021 - 28 Feb 2021
Virtual Venue

New Diagnostic Tests for Rotavirus and Norovirus Infections Evaluated

By LabMedica International staff writers
Posted on 21 Oct 2020
Print article
Image: The AFIAS-6 (AFIAS-automated fluorescent immunoassay system) is an automated fluorescent immunoassay system that uses blood, urine, and other samples to measure quantitatively or semi-quantitatively the concentration of targeted analyte (Photo courtesy of Boditech Med Inc).
Image: The AFIAS-6 (AFIAS-automated fluorescent immunoassay system) is an automated fluorescent immunoassay system that uses blood, urine, and other samples to measure quantitatively or semi-quantitatively the concentration of targeted analyte (Photo courtesy of Boditech Med Inc).
Acute gastroenteritis is one of the most impactful and common infectious diseases, accounting for millions of deaths annually in young children. Rotavirus and norovirus are leading causes of acute viral gastroenteritis spread through fecal to oral transmission.

For appropriate treatment and infection control, accurate and timely identification of pathogens is necessary. Various diagnostic tools including electron microscopy, latex agglutination, immunochromatographic assay (ICA), enzyme immunoassays, and molecular assays have been developed.

A team of Medical Laboratorians at the Samsung Medical Center (Seoul, Republic of Korea) used a total of 256 clinical stool samples submitted to the clinical microbiology laboratory at a tertiary referral hospital, from November 2018 to January 2019. After routine testing with multiplex real‐time RT‐PCR (rRT‐PCR), residual stool samples were stored at −70 °C prior to analysis.

The team evaluated the performance of Automated Fluorescent Immunoassay System ROTA (AFIAS‐Rota) and NORO (AFIAS‐Noro) assays (Boditech Med Inc, Chuncheon-si, Korea), newly developed automated fluorescent lateral flow immunoassays, in comparison with RIDASCREEN Rotavirus (RIDASCREEN‐Rota) and Norovirus (RIDASCREEN‐Noro) ELISA kits (R‐Biopharm, Darmstadt, Germany) for detection of rotavirus and norovirus. The Boditech assays were read after 12 minutes using that company’s AFIAS‐6 scanner and for the R-Biopharm assays the fluorescence was analyzed using a GEMINI spectrofluorometer (STRATEC Biomedical AG, Birkenfeld, Germany).

The investigators reported AFIAS‐Rota and RIDASCREEN Rotavirus had almost perfect agreement and substantial agreement was observed between AFIAS‐Noro and RIDASCREEN Norovirus. For detection of rotavirus, AFIAS and RIDASCREEN assays showed satisfactory diagnostic sensitivity (100% and 97.8%, respectively) and specificity (99.5% and 99.1%). For detection of norovirus, the RIDASCREEN assay showed significantly higher sensitivity than the AFIAS‐Noro (86.0% and 66.0%, respectively). Analytic specificity of AFIAS‐Rota/Noro assays showed no cross‐reactivity against any other bacteria (14 strains) or viruses (two strains). Hands‐on time (six minutes) and turnaround time (26 minutes) required to perform AFIAS assays were much shorter than those required for RIDASCREEN assays (20 and 150 minutes, respectively).

The authors concluded that the AFIAS‐Rota/Noro assays showed overall excellent agreement with the RIDASCREEN assays. Although the AFIAS‐Noro assay exhibited lower sensitivity than the RIDASCREEN Norovirus assay for detection of norovirus, the AFIAS‐Rota/Noro assays could be useful as a rapid initial screening test in clinical laboratories due to its convenience and rapid turnaround time. The study was published on September 23, 2020 in the Journal of Clinical Laboratory Analysis.

Related Links:
Samsung Medical Center
Boditech Med Inc
STRATEC Biomedical AG

Print article


Molecular Diagnostics

view channel
Image: Schematic representation of Chiari malformation type 1; it involves the lower part of the cerebellum known as tonsils, but not the brain stem (Photo courtesy of Healthline).

Common Brain Malformation Traced to Its Genetic Roots

About one in 100 children has a common brain disorder called Chiari 1 malformation, but most of the time such children grow up normally and no one suspects a problem. However about one in 10 of those children,... Read more


view channel
Image: uPath HER2 Dual ISH image analysis for breast cancer (Photo courtesy of Roche)

Roche Launches Digital Pathology Image Analysis Algorithms for Precision Patient Diagnosis in Breast Cancer

Roche (Basel, Switzerland) has announced the CE-IVD launch of its automated digital pathology algorithms, uPath HER2 (4B5) image analysis and uPath Dual ISH image analysis for breast cancer to help determine... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.