We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

EIKEN CHEMICAL

EIKEN CHEMICAL CO., LTD. provides clinical diagnostic aids, including biochemical, immunological, serological, dry ch... read more Featured Products: More products

Download Mobile App




Stool DNA Test Helps Detect Precancerous Lesions

By LabMedica International staff writers
Posted on 27 Dec 2019
Print article
Image: The cancer screening platform was a  multitarget stool DNA test (MT-sDNA), that uses multiple biomarkers to ensure the highest levels of sensitivity (Photo courtesy of Exact Sciences Laboratory)
Image: The cancer screening platform was a multitarget stool DNA test (MT-sDNA), that uses multiple biomarkers to ensure the highest levels of sensitivity (Photo courtesy of Exact Sciences Laboratory)
Colorectal cancer (CRC) is the third most common cancer and is one of the leading causes of cancer-related mortality. Although CRC incidence is increasing, a decrease in mortality has been observed due to the implementation of CRC screening and improved therapies.

CRC screening not only leads to a reduction in morbidity and mortality, but also to a lower CRC incidence when neoplastic lesions can be detected and removed at a premalignant stage. Therefore, CRC screening is considered cost-effective or even cost saving. Currently, colonoscopy is used for opportunistic screening programs in the USA, whereas in Europe, fecal immunochemical test (FIT) is the first choice for CRC screening in invitation-based screening programs.

Pathology scientists at the Netherlands Cancer Institute (Amsterdam, The Netherlands) and their colleagues prospectively collected 1,047 whole stool samples. Histology assessment was carried out and lesions were categorized as non-neoplastic, serrated polyp, adenoma, or carcinoma. Fecal immunochemical tests (FIT, OC-Sensor, Eiken Chemical, Tokyo, Japan) were performed by the screening participants before their preparation for screening colonoscopy.

A multitarget stool DNA test (MT-sDNA) was applied to all stool samples for DNA marker analysis that were collected and processed according to a uniform standard operational procedure and analyzed at Exact Sciences Laboratory (Madison, WI, USA). The team evaluated the efficacy of the MT-sDNA test that evaluates for KRAS mutations and NDRG4 and BMP3 promoter methylation in addition to testing for hemoglobin.

The team determined that the MT-sDNA test was more sensitive than FIT for the detection of precancerous lesions (46% versus 27%). While MT-sDNA detected 24 of 119 advanced lesions that were not detected by FIT, FIT detected just one precancerous lesion that was missed by MT-sDNA. The specificity for the MT-sDNA and FIT among individuals with non-advanced or negative findings was 89% and 93%, respectively. The two tests had similar sensitivity for detecting individuals with high-risk advanced adenomas from individuals with low-risk adenomas. A positive MT-sDNA test was associated with multiple lesions, larger lesions, and lesions with tubulovillous architecture.

The authors concluded that the MT-sDNA test combining DNA methylation and mutation markers with hemoglobin has a significantly increased sensitivity for advanced precancerous lesions than FIT alone at equal specificity, indicating its potential for application in population screening for CRC. Based on extensive cost-effectiveness evaluation, many organized screening programs use FIT as a triage test to select individuals for colonoscopy. Although detection of blood in stool has shown to be successful in reducing mortality from CRC, sensitivity can still be improved. The study was published in the December, 2019 issue of The American Journal of Gastroenterology.

Related Links:
Netherlands Cancer Institute
Eiken Chemical
Exact Sciences Laboratory


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: The study showed the blood-based cancer screening test detects 83% of people with colorectal cancer with specificity of 90% (Photo courtesy of Guardant Health)

Blood Test Shows 83% Accuracy for Detecting Colorectal Cancer

Colorectal cancer is the second biggest cause of cancer deaths among adults in the U.S., with forecasts suggesting 53,010 people might die from it in 2024. While fewer older adults are dying from this... Read more

Hematology

view channel
Image: The Gazelle Hb Variant Test (Photo courtesy of Hemex Health)

First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy

Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read more

Microbiology

view channel
Image: The new platform is designed to perform blood-based diagnoses of nontuberculosis mycobacteria (Photo courtesy of 123RF)

New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours

Breathing in nontuberculous mycobacteria (NTM) is a common experience for many people. These bacteria are present in water systems, soil, and dust all over the world and usually don't cause any problems.... Read more

Industry

view channel
Image: These new assays are being developed for use on the recently introduced DxI 9000 Immunoassay Analyzer (Photo courtesy of Beckman Coulter)

Beckman Coulter and Fujirebio Expand Partnership on Neurodegenerative Disease Diagnostics

Beckman Coulter Diagnostics (Brea, CA, USA) and Fujirebio Diagnostics (Tokyo, Japan) have expanded their partnership focused on the development, manufacturing and clinical adoption of neurodegenerative... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.