We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics

Download Mobile App




Events

ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

NanoString Technology Modernizes Liposarcoma Diagnostics

By LabMedica International staff writers
Posted on 15 Feb 2021
Print article
Image: Atypical lipomatous tumor/well-differentiated liposarcoma (left-hand); lipoma (right-hand) (Photo courtesy of Dr.Tony Ng, The University of British Columbia)
Image: Atypical lipomatous tumor/well-differentiated liposarcoma (left-hand); lipoma (right-hand) (Photo courtesy of Dr.Tony Ng, The University of British Columbia)
A recently published study showed that a newer NanoString-based method could allow for more rapid and cost-efficient diagnosis of liposarcomas than the commonly used fluorescence in situ hybridization (FISH) method.

Liposarcoma is a rare type of cancer that arises in fat cells in soft tissue, such as that inside the thigh. It is typically a large, bulky tumor, and tends to have multiple smaller satellites that extend beyond the main confines of the growth. Several types of liposarcoma exist. While some grow slowly with the cells remaining localized to one area of the body, other types grow very quickly and may metastasize.

In addition to FISH, which is a relatively expensive as well as labor- and equipment-intensive technology, immunohistochemistry (IHC) is often used to diagnose liposarcoma. However, IHC is felt to be inaccurate and hard to interpret.

To modernize the diagnosis of liposarcoma, investigators at the University of British Columbia (Vancouver, Canada) examined whether the newer NanoString-based technology (Seattle, WA, USA) could allow for more rapid and cost-efficient diagnosis of liposarcomas on standard formalin-fixed tissues through gene expression. For this study, they used large-scale transcriptome data from The Cancer Genome Atlas. The Cancer Genome Atlas is a project, begun in 2005, to catalog genetic mutations responsible for cancer, using genome sequencing and bioinformatics. The Cancer Genome Atlas applies high-throughput genome analysis techniques to improve diagnosis, treatment, and prevention of cancer through a better understanding of the genetic basis of this disease.

Data extracted from The Cancer Genome Atlas identified 20 genes, most from the 12q13-15 amplicon, that distinguished de-differentiated liposarcoma from other sarcomas and could be measured within a single NanoString assay. A machine learning model was subsequently developed to determine the probability that a given sample was positive for liposarcoma and was then applied to 45 retrospective cases to determine boundaries for positive and negative predictions. The effectiveness of the assay was validated on an independent set of 100 sarcoma samples (including 40 incident prospective cases), where histologic examination was considered insufficient for clinical diagnosis.

Results revealed that the NanoString assay had a 93% technical success rate, and an accuracy of 97.8% versus the FISH gold standard. Furthermore, results from the NanoString assay were available in 36 hours, whereas it required from one to two weeks to obtain FISH results.

"Liposarcomas are a type of malignant cancer that is difficult to diagnose because, even under a microscope, it is hard to differentiate liposarcomas from benign tumors or other types of cancer that need different treatments," said senior author Dr. Torsten Owen Nielsen, clinician-scientist in the department of pathology and laboratory medicine at the University of British Columbia. "Many liposarcomas look like their benign and relatively common counterparts, lipomas. Diagnostic delay and uncertainty cause severe stress for patients, and misdiagnosis can have many consequences including delayed or inadequate treatment or unnecessary surgical procedures and long-term postoperative follow up."

The liposarcoma-Nanostring study was published in the December 23, 2020 online edition of The Journal of Molecular Diagnosis.

Related Links:
University of British Columbia
NanoString


New
Gold Supplier
TBI Blood Test
i-STAT TBI Plasma Test
New
Silver Supplier
Immunoassay Reader
VEGA-200 Reader
New
Undercounter Laboratory Refrigerator
iLR105-GX
New
Nucleic Acid Purification System
CloNext 24

Print article

Channels

Clinical Chem.

view channel
Image: Illustration is of the Vertical Auto Profile (VAP) Lipid test with clear demarcation of the different lipoprotein classes and subclasses. (Photo courtesy of VAP Diagnostics Laboratory)

Lipoprotein(a) Concentrations Correlate With LDL-C in Diabetic Children

Cardiovascular disease (CVD) is a significant cause of mortality in those with diabetes. Increased apolipoprotein B (apoB) and low-density lipoprotein cholesterol (LDL-C) have been shown in pediatric patients... Read more

Hematology

view channel
Image: The UniCel DxH 800 Coulter Cellular Analysis System (Photo courtesy of Beckman Coulter)

Monocyte Distribution Width Predicts Sepsis in Critically Ill Patients

Sepsis has been reported as a major cause of increased morbidity, length of stay and mortality among patients hospitalized in Intensive Care Units (ICUs) for any cause. The survival of patients developing... Read more

Immunology

view channel
Image: Procartaplex Immunoassays Kits are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously (Photo courtesy of Thermo Fisher Scientific)

Assay Developed for Patient-Specific Monitoring and Treatment for Ovarian Cancer

Tumors can influence peripheral immune macroenvironment, thereby creating opportunities for non-invasive serum/plasma immunobiomarkers for immunostratification and immunotherapy designing.... Read more

Microbiology

view channel
Image: Clinical metagenomics (CMg) using nanopore sequencing (Photo courtesy of Oxford Nanopore Technologies)

Same Day Test Identifies Secondary Infections in COVID-19 Patients

The intensive care unit (ICU) is a dynamic environment with frequent staff-patient contact for invasive monitoring, interventions and personal care that together introduce the risk of secondary or nosocomial... Read more

Pathology

view channel
Image: The Ventana BenchMark Ultra autostainer is for cancer diagnostics with automation and the test menu include IHC, ISH, and FITC tests (Photo courtesy of Ventana Medical System)

Specific Biomarker Investigated for Triple-Negative Breast Cancer Diagnosis

Triple-negative breast cancer (TNBC) is defined by the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression and comprises a heterogeneous... Read more

Technology

view channel
Image: PKeye Workflow Monitor System (Photo courtesy of PerkinElmer, Inc.)

PerkinElmer’s New Cloud-Based Platform Enables Laboratory Personnel to Remotely Manage Its Instruments in Real-Time

PerkinElmer, Inc. (Waltham, MA; USA) has launched its PKeye Workflow Monitor, a cloud-based platform enabling laboratory personnel to remotely manage and monitor their PerkinElmer instruments and workflows... Read more

Industry

view channel
Illustration

Global HBA1c Laboratory Tests Market Driven by Rise in Diabetic Population

The global HBA1c laboratory tests market is projected to expand at a significant pace over the coming years, driven by an increase in the prevalence of diabetes, rise in prescription rate of HBA1c tests... Read more
Copyright © 2000-2021 Globetech Media. All rights reserved.