We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Infections Defined in Urothelial Bladder Tumors

By LabMedica International staff writers
Posted on 09 Aug 2018
Print article
Image: The Applied Biosystems 7500 real-time PCR System (Photo courtesy of Thermo Fisher Scientific).
Image: The Applied Biosystems 7500 real-time PCR System (Photo courtesy of Thermo Fisher Scientific).
Bladder cancer is estimated to be the 9th most common cancer worldwide and over 90% of bladder tumors are classed as urothelial bladder carcinomas (UBCs), with 75% to 80% presenting at non-muscle-invasive (NMIBC) stages and the remainder presenting at muscle-invasive (MIBC) and metastatic stages.

Recent sequencing studies have done much to unravel the enormous genomic complexity in UBCs; however, this explosion of knowledge has not yet led to the effective application of targeted therapies or a complete understanding of what causes the genomic aberrations that initiate and drive UBC.

Scientists at the University of Birmingham (Birmingham, UK) collected specimens from the primary UBCs of 689 patients recruited to the West Midlands Bladder Cancer Prognosis Programme (BCPP) between 2005 and 2011. The team used extracted DNA to screen for high-risk human papillomaviruses (HPV16 E6, HPV18 E7) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).

HPV16 and HPV18 were assayed in a multiplex quantitative polymerase chain reaction (qPCR) using primers and probes for HPV16 E6, HPV18 E7 and GAPDH using an Applied Biosystems 7500 Fast Real-Time PCR System. The team also used qPCR to screen for the BK virus (BKV), JC virus (JCV), or other polyomaviruses (HPyV). RNA from frozen specimens and cell lines was extracted using the RNeasy mini kit. Large T antigen immunohistochemistry was also performed.

The team reported that all 689 UBCs were negative for HPV18. One UBC from a female patient with areas of squamous differentiation was positive for HPV16. The qPCR data indicated variable levels of polyomavirus in 49 UBCs. In the UBCs with low Cts they were able to confirm that 23 were BKV and six were JCV by Sanger sequencing. Polyomavirus large T antigen expression was low but detectable in 70% of the sequencing-confirmed polyomavirus positive samples.

The authors concluded that in United Kingdom patients, the presence of HPV DNA sequences is extremely rare in UBC (<1% of cases). Polyomavirus DNA (predominantly BKV) is more common in UBC, but still only detectable in 7% of cases and in many of these cases at low copy number. Therefore HPV16, HPV18 and HPyV are unlikely to be common causative agents in UBC. The study was published on July 26, 2018, in the journal Scientific Reports.

Related Links:
University of Birmingham

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
Gold Member
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.