Whole Blood Collection Technology Enables Isolation of cfDNA, CTC, and RNA from Same Sample

RNA is a crucial tool in advancing our understanding of health and disease in humans and animals. However, RNA is particularly fragile and degrades quickly as soon the cells leave the internal environment of the body, such as when blood is drawn. A reliable technology to stabilize the sample right after collection is needed to reduce the degradation rate and maintain RNA integrity for proper analysis. Currently, efficient stabilization of RNA in body fluids, cells or tissues requires expensive cold chain involvement or relatively toxic fixative, which increases the cost per sample and urgency for processing the sample, leaving very little room for error. Whole blood can be a great source of stable RNA. However, commercially available whole blood collection tubes for RNA stabilization have certain limitations. Now, a groundbreaking product for ambient collection, stabilization, and transportation of whole blood samples enables isolation of cfDNA, CTC, and RNA from the same sample. Collected cells are maintained intact, making them also suitable for FACS analysis.

In order to overcome existing hurdles and enable widespread and efficient collection and analysis of whole blood, Mawi DNA Technologies (Hayward, CA, USA) has developed the HemaSure-OMICs Collection Tube products that offer non-toxic stabilization of whole blood, free of formaldehyde or formaldehyde-like ingredients. HemaSure-OMICs Whole Blood Collection Tubes are validated for room temperature stabilization of viral DNA and RNA for several days in viral load and detection assays applicable to the HIV, MERS-CoV, 2019-nCoV and other DNA and RNA viruses.

cfDNA (up to 14 days), CTC (up to 8 days), and RNA (up to 8 days) are stabilized at room temperature in the same blood tube (temperature range of 25° – 45° C). HemaSure-OMICs Whole Blood Collection Tubes are available in 3, 6, and 9 mL plastic vacuum filled blood collection tubes as well as a 250µl microtainer for blood drops for easy shipping and durability. They are compatible with any commercially available manual or automation-enabled kits for the purification of cfDNA and CTC from whole blood. HemaSure-OMICs Whole Blood Collection Tubes are for research use only and not for use in diagnostic or therapeutic procedures.

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