We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Technopath Clinical Diagnostics - An LGC Company


  Gold Agilent provides laboratories worldwide with instruments, services, consumables, applications and expertise, enabling... read more Featured Products: More products

Download Mobile App


ATTENTION: Due to the COVID-19 PANDEMIC, many events are being rescheduled for a later date, converted into virtual venues, or altogether cancelled. Please check with the event organizer or website prior to planning for any forthcoming event.

Cell-free RNA Profiles Gauge Preeclampsia Risk Months Before Symptoms

By LabMedica International staff writers
Posted on 18 Jan 2022
Print article
Image: The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries (Photo courtesy of Takara Bio)
Image: The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries (Photo courtesy of Takara Bio)
The period from conception to delivery represents the most rapid growth and development in an individual’s life. The ability to support this development requires dramatic and inadequately understood alterations in maternal physiology.

Preeclampsia, a pregnancy complication marked by the onset of hypertension, affects about 8% of pregnancies and contributes to maternal and neonatal morbidity and mortality. Signs of preeclampsia tend to arise late in pregnancy, and the disease is thought to originate with the establishment of the placenta in pregnancy.

A large team of clinical scientists including those at the Brigham and Women’s Hospital (Boston, MA, USA) and Mirvie, Inc., (South San Francisco, CA, USA) demonstrated the ability of plasma cell-free RNA (cfRNA) to reveal patterns of normal pregnancy progression and determine the risk of developing pre-eclampsia months before clinical presentation. The team gathered maternal transcriptome data from eight different prospective cohorts, pulling together data that encompassed 2,539 plasma samples and 1,840 pregnancies. This cohort included women of a range of ethnic, national, geographical, and socioeconomic backgrounds.

In particular, the scientists conducted a case-control study of 72 individuals with preeclampsia and 452 controls, including individuals with chronic hypertension and gestational hypertension using blood samples taken during the second trimester. They performed PCR with reverse transcription (RT–qPCR) analysis to assess the relative amount of cfRNA extracted from each sample. They measured and compared the threshold cycle (Ct) values from each RNA sample using a three-color multiplex qPCR assay. cfRNA libraries were prepared using the SMARTer Stranded Total RNAseq-Pico Input Mammalian kit (Takara Bio, Kusatsu, Japan). RNA measurements and fragment analysis was performed on a Fragment Analyzer 5300 (Agilent Technologies, San Diego, CA, USA).

The investigators identified seven genes whose signatures consistently separated cases and controls. Four of the genes, PAPPA2, CLDN7, TLE6, and FABP1, have functions linked to preeclampsia or to placental development and the three others, SNORD14A, PLEKHH1, and MAGEA10, have been tied bioinformatically to preeclampsia, though their functions are unclear.

At a sensitivity of 75%, a model based on these gene signatures had a positive predictive value of 32.3%, with a preeclampsia prevalence of 13.7% in the study. This, according to the team, is better than currently used clinical models, which have positive predictive values of 4.4%, and rely on maternal factors. The test also detected preeclampsia risk early in pregnancy. It correctly identified 73% of expectant mothers, who later had a medically indicated preterm birth three months before the onset of clinical symptoms or delivery.

The authors concluded that their findings can now be leveraged to more accurately provide information on future maternal and fetal health and disease. Thus, their approach opens new therapeutic windows to effectively decrease maternal and neonatal morbidity and mortality. The study was published on January 5, 2022 in the journal Nature.

Related Links:
Brigham and Women’s Hospital
Mirvie, Inc
Takara Bio
Agilent Technologies

Gold Supplier
Immunofluorescence Analyzer
Getein 1160
DP0150 Pulse 150
Chemistry Analyzer
Silver Supplier
3-Part Diff Hematology Analyzer
Abacus Junior 30

Print article


Clinical Chem.

view channel
Image: The analysis pipeline used to investigate associations between blood metabolites, later life brain imaging measures, and genetic risk for Alzheimer’s disease (Photo courtesy of University College London)

Lipid Measurements Show Potential as Alzheimer’s Disease Biomarkers

Brain changes accompanying ageing are varied and can include pathologies that lead to cognitive impairment, the commonest of which is Alzheimer’s disease (AD). Identifying blood-based signatures of brain... Read more


view channel
Image: The CS-2500 analyzer features pre-analytic sample checks and four detection methods simultaneously on a single platform – coagulation end-point, chromogenic kinetic analysis, turbidimetric immunoassay and automated platelet aggregation (Photo courtesy of Sysmex)

Microvascular/Endothelial Dysfunction Contributes to Post-COVID Syndrome Pathogenesis

Post-COVID syndrome (PCS) or Long-COVID is an increasingly recognized complication of acute SARS-CoV-2 infection, characterized by persistent fatigue, reduced exercise tolerance chest pain, shortness of... Read more


view channel
Image: Sales of lateral flow assays in clinical testing are expected to register a CAGR of 5% through 2032 (Photo courtesy of Pexels)

Global Lateral Flow Assays Market to Surpass USD 11.5 Billion by 2032 Due to Evolving Applications

The global lateral flow assays market was valued at USD 7.2 billion in 2021 and is projected to register a CAGR of 4.7% during 2022-2032 to surpass USD 11.7 billion by the end of 2032, driven by the growing... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.