We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Breast Cancer Histopathology Employs Infrared Spectroscopic Imaging

By LabMedica International staff writers
Posted on 19 Apr 2021
Digital analysis of cancer specimens using spectroscopic imaging coupled to machine learning is an emerging area that links spatially localized spectral signatures to tissue structure and disease. More...
Breast histopathology, as an example of the broad relevance of these techniques, is critically important for clinical diagnoses.

Current histologic characterization is morphology-based; thin tissue sections are stained, and cells are visually recognized by a pathologist using an optical microscope. However, the basis of the disease is well known to be molecular. Molecular analysis for pathology is complicated by the spatial diversity of cells and acellular materials, necessitating an analytical technique that involves imaging.

Bioengineers at the University of Illinois at Urbana−Champaign (Urbana, IL, USA) and their colleagues examined the role of spatial-spectral tradeoffs in infrared spectroscopic imaging configurations for probing tumors and the associated microenvironment profiles at different levels of model complexity. The imaged breast tissue using standard and high-definition Fourier Transform Infrared (FT-IR) imaging and systematically examine the localization, spectral origins, and utility of data for classification.

The team obtained formalin-fixed, paraffin-embedded serial breast tissue microarrays (TMA) sections. The array consisted of a total of 101 cores of 1 mm diameter from 47 patients. Two sections were stained with hematoxylin and eosin (H&E) and other immunohistochemical markers and imaged with a light microscope. High-definition (HD) FT-IR imaging was conducted using the Agilent Stingray imaging system (Santa Clara, CA, USA) which is comprised of a 680-IR spectrometer coupled to a 620-IR imaging microscope with 0.62 numerical aperture, 25×objective.

The scientists provided a systematic comparison in the use of HD and SD FT-IR imaging data for breast pathology in their study. While the increased spatial localization of spectral signals in HD imaging may have been expected to provide a confounding influence, the study demonstrated that accuracy can be high, and there is significant potential in this sampling mode offering higher sensitivity. The team stated that IR imaging can not only provide the recognition capability of molecular data but can also balance that with an increased quality of morphologic data.

Rohit Bhargava, PhD, bioengineering professor and senior author of the study, said, “As technology expands and provides more capabilities with new features, it becomes more difficult to choose the optimal technology from the many options available. This study provides a nice comparison and guidelines to design a more useful and practical technology.” The study was originally published on February 27, 2021 in the journal Clinical Spectroscopy.

Related Links:
University of Illinois at Urbana−Champaign


Gold Member
Antipsychotic TDM Assays
Saladax Antipsychotic Assays
Serological Pipet Controller
PIPETBOY GENIUS
New
Automatic Hematology Analyzer
LABAS F9000
New
Rapid Test Reader
DIA5000
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Hematology

view channel
Image: CitoCBC is the world first cartridge-based CBC to be granted CLIA Waived status by FDA (Photo courtesy of CytoChip)

Disposable Cartridge-Based Test Delivers Rapid and Accurate CBC Results

Complete Blood Count (CBC) is one of the most commonly ordered lab tests, crucial for diagnosing diseases, monitoring therapies, and conducting routine health screenings. However, more than 90% of physician... Read more

Immunology

view channel
Image: A simple blood test could replace surgical biopsies for early detecion of heart transplant rejection (Photo courtesy of Shutterstock)

Blood Test Detects Organ Rejection in Heart Transplant Patients

Following a heart transplant, patients are required to undergo surgical biopsies so that physicians can assess the possibility of organ rejection. Rejection happens when the recipient’s immune system identifies... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.