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新的方案快速检测三种军团菌

By LabMedica International staff writers
Posted on 10 May 2016
现已开发出一种创新的方案,用于检测三种最常见的军团菌菌种,能在8小时内判断出感染源。

军团菌属是一组革兰氏阴性菌病原菌群,包括引发军团杆菌病的嗜肺军团菌,以及引发包括被称为军团杆菌性肺炎的肺炎型疾病和被称为庞蒂亚克热的轻度流感样疾病的军团杆菌。

Polytechnic University of Valencia(UPV;Spain)的科学家依据一项日本研究组的调查开展了一项研究,旨在同时检测多达4种军团菌。但是,UPV团队发现,这种方法不可行,开始开发一种同时检测三种军团菌的方法,这三种军团菌是:嗜肺性军团菌(是90%人类军团菌病例的致病菌)、麦氏军团菌和长滩军团菌。该方案被视为一种突破,依靠联合使用多重聚合酶链式反应(多重PCR)和叠氮溴化丙锭(PMA)前处理。

新方案令检测时间从14天降到仅需8小时,令最常使用的检测方法有了明显的改善。新方案还能成功用于各种污染样品,现有方法不能实现此类用途。Yolanda Moreno Trigo博士,项目的高级研究员说明,“我们浓缩样品,破开细菌,以便提取其DNA,然后使用多重PCR鉴别不同军团菌属致病菌。多重PCR扩增目标属的DNA,更容易鉴别目标种属。使用PMA前处理允许我们立即放弃死细胞的DNA,我们不关注后者。”

Related Links:
Polytechnic University of Valencia 

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