Lipid Micelles May Replace Viruses for Gene Therapy

Another method, using DNA-lipid micelles, avoids problems of immune responses to the viral agent but is only being tested in about 10% of clinical trials. The main reason for this has been the lower efficiency of the micelles as compared to the viral vectors.

Investigators at the University of California, Santa Barbara (USA), have added a novel lipid molecule to the pool available for synthesis of DNA-lipid micelles. The molecule contains a highly charged (16+) multivalent cationic lipid, MVLBG2, with a large, highly branched nanoscale head group. The unusually large head group allows "honeycomb” interaction between the lipid and DNA molecules.

The investigators used their lipid-DNA micelles to transfect two lines of mouse and two lines of human tissue culture cells. One of the lines was comprised of mouse embryonic fibroblasts (MEF) that are notoriously difficult to transfect.

Results published in the March 29, 2006, issue of the Journal of the American Chemical Society revealed that the honeycomb structure of the lipid head group greatly enhanced the micelle's ability to transfer genetic material to the target cell. Senior author Dr. Cyrus R. Safinya, professor of molecular, cellular, and developmental biology at the University of California, Santa Barbara, said, Our data confirm that MEFs are generally hard to transfect. And the new molecule is far superior for transfection of these cells as compared to commercial lipids.”




Related Links:
University of California, Santa Barbara