New Fluorescence Assays for Molecular Biology

These include separate, ready-for-use kits for measuring DNA, RNA, and protein.

The new fluorescence assays comprise the Quant-iTT line marked by Molecular Probes (Eugene, OR USA). Separate kits are available for DNA, RNA, and protein. The fluorescent dye in each Quant-iT kit matches common fluorescence excitation and emission filter sets in fluorescent microplate readers.

The DNA kit is described as being several orders of magnitude more sensitive than UV absorbance methods while requiring only one to 20 µl of sample. The wide assay range eliminates the need to dilute concentrated samples.

The RNA kit is cited as being the first homogenous assay ever developed for quantitation of RNA in the presence of DNA. Molecular Probes says that the protein assay is easier to perform and more sensitive than standard absorbance based assays. The signal is unaffected by many common contaminants including DTT, beta-mercaptoethanol, amino acids, and DNA.




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Molecular Probes