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血清试管减少了假阳性肌钙蛋白结果

By LabMedica International staff writers
Posted on 12 Nov 2012
研究人员比较了用快速血清试管(RST)和血浆分离试管(PST)收集血样进行心肌钙蛋白分析的优劣。

血浆样本常常用于心肌钙蛋白检验,因为它的运行时间比血清分离管更短(SST),后者最长需要30分钟才能凝结。

梅奥诊所(Rochester MN, USA; www.mayoclinic.org)的科学家测量了443名急诊患者的心肌钙蛋白(cTnT)浓度,这些患者接受了一组心脏生物标记物的检验,并被收集了RST和PST样本。在一台罗氏Cobas e411分析仪(Roche Diagnostics, Indianapolis, IN, USA; www.rocheusa.com)上用第4代罗氏肌钙蛋白T STAT测定进行的全部的cTnT分析。

所用的血清RST和血浆PST收集试管是碧迪公司(Franklin Lakes, NJ, USA; www.bd.com)的产品。研究人员确定了RST和PST cTnT浓度之间的平均偏差以及RST和PST结果的临床一致性。临床一致的结果定义为:cTnT结果或者在0.01纳克/毫升以下不可检测,或者,如果在大于等于0.01纳克/毫升时能检测到,样本类型之间的差值大于等于0.02纳克/毫升。

443份样本中的328份样本在RST和PST试管中都检测不到cTnT浓度。7份样本在PST试管中能检测到cTnT结果,但在同时收集的RST试管中检测不到。用快速凝结血清试管没有观察到锂肝素样本的罕见假阳性结果。443份样本中的10份样本在PST和RST试管里观察到临床不一致的cTnT结果,其中三个cTnT结果高到了如果不用PST或RST样本的cTnT结果诊断就不会受影响的程度。

该研究的论文发表于2012年7月号的《临床生物化学》(Clinical Biochemistry)杂志。作者总结说,RST试管收集的样本得到的cTnT结果在分析上和临床上与PST试管收集的样本一致。与贝克曼库尔特(Brea, CA, USA; www.beckmancoulter.com)平台上进行的肌钙蛋白I研究类似,他们发现用RST收集心脏生物标记物样本也许能减少假阳性的结果的发生。

Related Links:
Mayo Clinic >>> www.mayoclinic.org
Roche Diagnostics >>> www.rocheusa.com
Becton–Dickinson and Company >>> www.bd.com
Beckman–Coulter >>> www.beckmancoulter.com
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