Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
RANDOX LABORATORIES

Events

02 Oct 2016 - 05 Oct 2016
10 Oct 2016 - 12 Oct 2016

Accurate Epigenetic Test Eliminates Superfluous Biopsies for Prostate Cancer

By Labmedica International staff writers
Posted on 06 Aug 2014
Print article
Image: The photomicrograph shows the appearance of a low grade carcinoma of the prostate that can be difficult to recognize as malignancy, particularly in small needle biopsies (Photo courtesy of the University of Washington).
Image: The photomicrograph shows the appearance of a low grade carcinoma of the prostate that can be difficult to recognize as malignancy, particularly in small needle biopsies (Photo courtesy of the University of Washington).
Two independent trials have now validated the performance of an epigenetic test that could provide physicians with a better tool to help eliminate unnecessary repeat prostate biopsies.

Patients with a negative biopsy have been evaluated with an epigenetic test to identify those at low risk for harboring cancer missed through biopsy sampling error who could forego an unnecessary repeat biopsy.

Scientists at the James Buchanan Brady Urological Institute (Baltimore, MD, USA) working with colleagues from other institutes, evaluated the archived, cancer negative prostate biopsy core tissue samples of 350 subjects from a total of five urological centers in the USA. All subjects underwent repeat biopsy within 24 months with a negative (controls) or positive (cases) histopathological result. Centralized blinded pathology evaluation of the two biopsy series was performed in all available samples from each site.

Formalin fixed, paraffin embedded tissue cores were epigenetically profiled based on three genes: glutathione S-transferase pi (GSTP1), adenomatous polyposis coli (APC) and Ras association (RalGDS/AF-6) domain family 1 (RASSF1). GSTP1 methylation is a specific biomarker for prostate cancer and this gene is methylated in up to 90% of prostate cancer cases. Additionally, APC and RASSF1 are important field effect markers and increase the diagnostic sensitivity of the assay. Epigenetic analysis was performed in random, blinded fashion using a multiplex methylation specific polymerase chain reaction (MDxHealth; Irvine, CA, USA).

After correcting for age, prostate specific antigen (PSA), digital rectal exam, histopathological characteristics of the first biopsy, and race, this epigenetic test proved to be the most significant, independent, and strongest predictor of patient outcome with an odds ratio of 2.69 as well as the most valuable diagnostic aid of all evaluated risk factors. Alan W. Partin, MD, PhD, the lead author of the study said, “This epigenetic assay is a significant, independent predictor and has been shown to be the most valuable diagnostic aid of all evaluated risk factors in two independent trials. Negative findings of this assay could be used to reduce concern over unsampled cancer and effectively avoid unnecessary repeat biopsies.” The study was published online on April 18, 2014, in the Journal of Urology.

Related Links:

James Buchanan Brady Urological Institute
MDxHealth



Print article
Sekisui Diagnostics

Channels

Industry News

view channel

Instrumentation Laboratory Acquires CA Casyso

Instrumentation Laboratory (IL) (Bedford, MA, USA), which manufactures instruments and reagents for the global hemostasis market, has acquired CA Casyso AG (Basel, Switzerland) and its Tem subsidiaries (Tem), a provider of in vitro diagnostic (IVD) testing for patient blood management (PBM). CA Casyso AG is the parent... Read more
Copyright © 2000-2016 Globetech Media. All rights reserved.